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Literature summary extracted from

  • Ying, X.; Wang, Y.; Xiong, B.; Wu, T.; Xie, L.; Yu, M.; Wang, Z.
    Characterization of an allylic/benzyl alcohol dehydrogenase from Yokenella sp. strain WZY002, an organism potentially useful for the synthesis of alpha,beta-unsaturated alcohols from allylic aldehydes and ketones (2014), Appl. Environ. Microbiol., 80, 2399-2409.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.1 dithiothreitol 1 mM, 123% of initial activity Yokenella sp.
1.1.1.1 EDTA 1 mM, 110% of initial activity Yokenella sp.
1.1.1.91 acetone 125% activity at 20% (v/v) acetone Yokenella sp. WZY002
1.1.1.91 dithiothreitol 123% activity at 1 mM Yokenella sp. WZY002
1.1.1.91 EDTA 110% activity at 1 mM Yokenella sp. WZY002

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expression in Escherichia coli Yokenella sp.
1.1.1.90 expressed in Escherichia coli BL21(DE3) cells Yokenella sp. WZY002
1.1.1.91 expressed in Escherichia coli BL21(DE3) cells Yokenella sp. WZY002

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 Ag+ 1 mM, no residual activity Yokenella sp.
1.1.1.1 Co2+ 1 mM, 19% residual activity Yokenella sp.
1.1.1.1 Cu2+ 1 mM, no% residual activity Yokenella sp.
1.1.1.1 Fe2+ 1 mM, 60% residual activity Yokenella sp.
1.1.1.1 Zn2+ 1 mM, 0.5% residual activity Yokenella sp.
1.1.1.90 Ag+
-
 Yokenella sp. WZY002
1.1.1.90 Cu2+
-
 Yokenella sp. WZY002
1.1.1.90 Zn2+
-
 Yokenella sp. WZY002
1.1.1.91 Ag+ complete inhibition at 1 mM Yokenella sp. WZY002
1.1.1.91 Al3+ 13.5% inhibition at 1 mM Yokenella sp. WZY002
1.1.1.91 Co2+ 80.5% inhibition at 1 mM Yokenella sp. WZY002
1.1.1.91 Cu2+ complete inhibition at 1 mM Yokenella sp. WZY002
1.1.1.91 Fe2+ 39.7% inhibition at 1 mM Yokenella sp. WZY002
1.1.1.91 iodoacetate 94.5% residual activity at 10 mM Yokenella sp. WZY002
1.1.1.91 Zn2+ 99.5% inhibition at 1 mM Yokenella sp. WZY002

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.4
-
 benzaldehyde pH 6.5, 65°C Yokenella sp.
1.1.1.1 0.6
-
 NADPH pH 6.5, 65°C Yokenella sp.
1.1.1.1 0.7
-
 NADP+ pH 8.0, 55°C Yokenella sp.
1.1.1.1 3.3
-
 (2E)-but-2-enal pH 6.5, 65°C Yokenella sp.
1.1.1.1 9.1
-
 (2E)-but-2-en-1-ol pH 8.0, 55°C Yokenella sp.
1.1.1.1 14
-
 benzyl alcohol pH 8.0, 55°C Yokenella sp.
1.1.1.91 0.4
-
 benzaldehyde at pH 6.5 and 65°C Yokenella sp. WZY002
1.1.1.91 0.6
-
 NADP+ with crotonaldehyde as cosubstrate, at pH 8.0 and 55°C Yokenella sp. WZY002
1.1.1.91 0.7
-
 NADP+ with crotyl alcohol as cosubstrate, at pH 8.0 and 55°C Yokenella sp. WZY002
1.1.1.91 3.3
-
 crotonaldehyde at pH 8.0 and 55°C Yokenella sp. WZY002
1.1.1.91 9.1
-
 crotonyl alcohol at pH 8.0 and 55°C Yokenella sp. WZY002
1.1.1.91 14
-
 benzyl alcohol at pH 6.5 and 65°C Yokenella sp. WZY002

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Ba2+ 1 mM, 113% of initial activity Yokenella sp.
1.1.1.90 Zn2+ dependent on Yokenella sp. WZY002
1.1.1.91 Ba2+ 113% activity at 1 mM Yokenella sp. WZY002
1.1.1.91 additional information Ca2+, Na+, Mg2+, Mn2+, and K+ have no significant effect on the enzyme activity Yokenella sp. WZY002
1.1.1.91 Zn2+ dependent on Yokenella sp. WZY002

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 36411
-
 x * 36411, calculated, x * 37000, SDS-PAGE Yokenella sp.
1.1.1.1 37000
-
 x * 36411, calculated, x * 37000, SDS-PAGE Yokenella sp.
1.1.1.90 36411
-
-
 Yokenella sp. WZY002
1.1.1.91 36411
-
-
 Yokenella sp. WZY002
1.1.1.91 39000
-
 2 * 39000, SDS-PAGE Yokenella sp. WZY002
1.1.1.91 75000
-
 gel filtration Yokenella sp. WZY002

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.1 DMSO 20% v/v, 24 h, 87% residual activity Yokenella sp.
1.1.1.90 DMSO the enzyme retains 87.5% of the initial activity after 24 h of incubation with 20% (v/v) dimethyl sulfoxide Yokenella sp. WZY002
1.1.1.91 DMSO the enzyme retains 87.5% of the initial activity after 24 h of incubation with 20% (v/v) dimethyl sulfoxide and also remains active in 40% (v/v) Yokenella sp. WZY002
1.1.1.91 Ethanol the enzyme retains more than 49% of the initial activity in 20% (v/v) ethanol and also remains active in 40% (v/v) Yokenella sp. WZY002
1.1.1.91 isopropanol the enzyme retains more than 49% of the initial activity in 20% (v/v) isopropanol and also remains active in 40% (v/v) Yokenella sp. WZY002
1.1.1.91 Methanol the enzyme retains more than 49% of the initial activity in 20% (v/v) methanol and also remains active in 40% (v/v) Yokenella sp. WZY002

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Yokenella sp. W6CX26
-
-
1.1.1.90 Yokenella sp. WZY002 W6CX26
-
-
1.1.1.91 Yokenella sp. WZY002 W6CX26
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Yokenella sp.
1.1.1.91 DEAE Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration Yokenella sp. WZY002

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 (2E)-2-methylpent-2-enal + NADPH + H+ 41.5% of the activity with (2E)-but-2-enal, yield 95% after 12 h Yokenella sp. (2E)-2-methylpent-2-en-1-ol + NADP+
-
 ?
1.1.1.1 (2E)-3,7-dimethylocta-2,6-dienal + NADPH + H+ 37.4% of the activity with (2E)-but-2-enal, yield 87% after 12 h Yokenella sp. (2E)-3,7-dimethylocta-2,6-dien-1-ol + NADP+
-
 ?
1.1.1.1 (2E)-but-2-en-1-ol + NADP+
-
 Yokenella sp. (2E)-but-2-enal + NADPH + H+
-
 r
1.1.1.1 (2E)-but-2-enal + NADPH + H+ yield 96% after 12 h Yokenella sp. (2E)-but-2-en-1-ol + NADP+
-
 r
1.1.1.1 (2E)-dec-2-enal + NADPH + H+ 16.7% of the activity with (2E)-but-2-enal, yield 91% after 12 h Yokenella sp. (2E)-dec-2-en-1-ol + NADP+
-
 ?
1.1.1.1 (2E)-hex-2-enal + NADPH + H+ 41.7% of the activity with (2E)-but-2-enal, yield 98% after 12 h Yokenella sp. (2E)-hex-2-en-1-ol + NADP+
-
 ?
1.1.1.1 (2E)-oct-2-enal + NADPH + H+ 30.2% of the activity with (2E)-but-2-enal, yield 69% after 12 h Yokenella sp. (2E)-oct-2-en-1-ol + NADP+ plus 27% oct-2-enyl ester ?
1.1.1.1 benzaldehyde + NADPH + H+ 107% of the activity with (2E)-but-2-enal, yield 99% after 12 h Yokenella sp. benzyl alcohol + NADP+
-
 r
1.1.1.1 benzyl alcohol + NADP+
-
 Yokenella sp. benzaldehyde + NADPH + H+
-
 r
1.1.1.1 additional information enzyme selectively reduces the C=O bond of allylic aldehydes/ketones to the corresponding alpha,beta-unsaturated alcohols and also has the capacity of stereoselectively reducing aromatic ketones to (S)-enantioselective alcohols. The enzyme preferentially catalyzes oxidation of allylic/benzyl aldehydes Yokenella sp. ?
-
 ?
1.1.1.90 benzaldehyde + NADPH + H+ highest reductive activity with benzaldehyde Yokenella sp. WZY002 benzyl alcohol + NADP+
-
 r
1.1.1.90 benzyl alcohol + NADP+
-
 Yokenella sp. WZY002 benzaldehyde + NADPH + H+
-
 r
1.1.1.90 crotonaldehyde + NADPH + H+
-
 Yokenella sp. WZY002 crotonyl alcohol + NADP+
-
 r
1.1.1.90 crotonyl alcohol + NADP+ highest oxidative activity with crotyl alcohol Yokenella sp. WZY002 crotonaldehyde + NADPH + H+
-
 r
1.1.1.90 additional information no activity with NADH or NAD+ Yokenella sp. WZY002 ?
-
 ?
1.1.1.91 (E)-oct-2-enal + NADPH + H+
-
 Yokenella sp. WZY002 trans-2-octen-1-ol + NADP+
-
 ?
1.1.1.91 1-butanol + NADP+ 56% activity compared to crotyl alcohol Yokenella sp. WZY002 butyraldehyde + NADPH + H+
-
 ?
1.1.1.91 1-propanol + NADP+ 17.4% activity compared to crotyl alcohol Yokenella sp. WZY002 propionaldehyde + NADPH + H+
-
 ?
1.1.1.91 2-hydroxyacetophenone + NADPH + H+
-
 Yokenella sp. WZY002 (S)-1,2-phenylethanediol + NADP+
-
 ?
1.1.1.91 acetophenone + NADPH + H+
-
 Yokenella sp. WZY002 ?
-
 r
1.1.1.91 alpha-ionone + NADPH + H+
-
 Yokenella sp. WZY002 ?
-
 r
1.1.1.91 benzaldehyde + NADPH + H+ highest reductive activity with benzaldehyde Yokenella sp. WZY002 benzyl alcohol + NADP+
-
 r
1.1.1.91 benzyl alcohol + NADP+ 41.7% activity compared to crotyl alcohol Yokenella sp. WZY002 benzaldehyde + NADPH + H+
-
 r
1.1.1.91 crotonaldehyde + NADPH + H+
-
 Yokenella sp. WZY002 crotonyl alcohol + NADP+
-
 r
1.1.1.91 crotonyl alcohol + NADP+ highest oxidative activity with crotyl alcohol Yokenella sp. WZY002 crotonaldehyde + NADPH + H+
-
 r
1.1.1.91 additional information no activity with NADH or NAD+ Yokenella sp. WZY002 ?
-
 ?
1.1.1.91 trans-4-phenyl-3-buten-2-one + NADPH + H+
-
 Yokenella sp. WZY002 ?
-
 r
1.1.1.91 vanillin + NADPH + H+
-
 Yokenella sp. WZY002 vanillic alcohol + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.1 ? x * 36411, calculated, x * 37000, SDS-PAGE Yokenella sp.
1.1.1.90 ? x * 36411, calculated from amino acid sequence Yokenella sp. WZY002
1.1.1.91 homodimer 2 * 39000, SDS-PAGE Yokenella sp. WZY002
1.1.1.91 homodimer 2 * 36411, calculated from amino acid sequence Yokenella sp. WZY002

Synonyms

EC Number Synonyms Comment Organism
1.1.1.90 ADH
-
 Yokenella sp. WZY002
1.1.1.91 ADH
-
 Yokenella sp. WZY002

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 55
-
 oxidation of crotyl aclohol Yokenella sp.
1.1.1.1 65
-
 reduction of crotonaldehyde Yokenella sp.
1.1.1.90 55
-
 for crotonyl alcohol oxidation Yokenella sp. WZY002
1.1.1.90 65
-
 for crotonaldehyde reduction Yokenella sp. WZY002
1.1.1.91 55
-
 for crotonyl alcohol oxidation Yokenella sp. WZY002
1.1.1.91 65
-
 for crotonaldehyde reduction Yokenella sp. WZY002

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 55
-
 half-life 6.2 h Yokenella sp.
1.1.1.90 55
-
 the enzyme shows moderate thermostability with a half-life of 6.2 h at 55°C Yokenella sp. WZY002
1.1.1.91 55 65 the enzyme shows moderate thermostability with a half-life of 6.2 h at 55°C and 1.5 h at 60°C. The activity loss at 65°C is relatively rapid, and the enzyme retains 30.8% of the initial activity after 30 min of heat treatment Yokenella sp. WZY002

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 93.3
-
 benzyl alcohol pH 8.0, 55°C Yokenella sp.
1.1.1.1 101
-
 (2E)-but-2-en-1-ol pH 8.0, 55°C Yokenella sp.
1.1.1.1 194
-
 NADP+ pH 8.0, 55°C Yokenella sp.
1.1.1.1 360
-
 benzaldehyde pH 6.5, 65°C Yokenella sp.
1.1.1.1 405
-
 (2E)-but-2-enal pH 6.5, 65°C Yokenella sp.
1.1.1.1 433
-
 NADPH pH 6.5, 65°C Yokenella sp.
1.1.1.91 93.3
-
 benzyl alcohol at pH 6.5 and 65°C Yokenella sp. WZY002
1.1.1.91 101
-
 crotonyl alcohol at pH 8.0 and 55°C Yokenella sp. WZY002
1.1.1.91 194
-
 NADP+ with crotyl alcohol as cosubstrate, at pH 8.0 and 55°C Yokenella sp. WZY002
1.1.1.91 360
-
 benzaldehyde at pH 6.5 and 65°C Yokenella sp. WZY002
1.1.1.91 405
-
 crotonaldehyde at pH 8.0 and 55°C Yokenella sp. WZY002
1.1.1.91 433
-
 NADP+ with crotonaldehyde as cosubstrate, at pH 8.0 and 55°C Yokenella sp. WZY002

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 6.5
-
 reduction of crotonaldehyde Yokenella sp.
1.1.1.1 8
-
 oxidation of crotyl aclohol Yokenella sp.
1.1.1.90 6.5
-
 for crotonaldehyde reduction Yokenella sp. WZY002
1.1.1.90 8
-
 for crotonyl alcohol oxidation Yokenella sp. WZY002
1.1.1.91 6.5
-
 for crotonaldehyde reduction Yokenella sp. WZY002
1.1.1.91 8
-
 for crotonyl alcohol oxidation Yokenella sp. WZY002

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 additional information no activity with NAD(H) Yokenella sp.
1.1.1.1 NADPH enzyme strictly requires NADP(H) as a coenzyme Yokenella sp.
1.1.1.90 NADP+
-
 Yokenella sp. WZY002
1.1.1.90 NADPH
-
 Yokenella sp. WZY002
1.1.1.91 NADP+
-
 Yokenella sp. WZY002
1.1.1.91 NADPH
-
 Yokenella sp. WZY002

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.1 6.6
-
 benzyl alcohol pH 8.0, 55°C Yokenella sp.
1.1.1.1 11
-
 (2E)-but-2-en-1-ol pH 8.0, 55°C Yokenella sp.
1.1.1.1 122
-
 (2E)-but-2-enal pH 6.5, 65°C Yokenella sp.
1.1.1.1 278
-
 NADP+ pH 8.0, 55°C Yokenella sp.
1.1.1.1 720
-
 NADPH pH 6.5, 65°C Yokenella sp.
1.1.1.1 899
-
 benzaldehyde pH 6.5, 65°C Yokenella sp.
1.1.1.91 6.6
-
 benzyl alcohol at pH 6.5 and 65°C Yokenella sp. WZY002
1.1.1.91 11
-
 crotonyl alcohol at pH 8.0 and 55°C Yokenella sp. WZY002
1.1.1.91 122
-
 crotonaldehyde at pH 8.0 and 55°C Yokenella sp. WZY002
1.1.1.91 278
-
 NADP+ with crotyl alcohol as cosubstrate, at pH 8.0 and 55°C Yokenella sp. WZY002
1.1.1.91 720
-
 NADP+ with crotonaldehyde as cosubstrate, at pH 8.0 and 55°C Yokenella sp. WZY002
1.1.1.91 899
-
 benzaldehyde at pH 6.5 and 65°C Yokenella sp. WZY002