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Literature summary extracted from

  • Dahncke, K.; Witte, C.P.
    Plant purine nucleoside catabolism employs a guanosine deaminase required for the generation of xanthosine in Arabidopsis (2013), Plant Cell, 25, 4101-4109.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.15 expressed in Nicotiana benthamiana Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.15 0.264
-
 guanosine at pH 8.0 and 40°C Arabidopsis thaliana
3.5.4.15 0.5761
-
 2'-deoxyguanosine at pH 8.0 and 40°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.4.15 cytosol
-
 Arabidopsis thaliana 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.15 45000
-
 x * 45000, enzyme-yellow fluorescent protein complex, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.15 2'-deoxyguanosine + H2O Arabidopsis thaliana
-
 2'-deoxyxanthosine + NH3
-
 ?
3.5.4.15 guanosine + H2O Arabidopsis thaliana
-
 xanthosine + NH3
-
 ?
3.5.4.15 additional information Arabidopsis thaliana no activity with adenine, adenosine, AMP, guanine, GMP, cytosine, cytidine, CMP, cyclic GMP, pterine, and tetrahydrofolate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.15 Arabidopsis thaliana
-
 ecotype Col-0
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.15 affinity column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.15 flower
-
 Arabidopsis thaliana
-
3.5.4.15 leaf
-
 Arabidopsis thaliana
-
3.5.4.15 root
-
 Arabidopsis thaliana
-
3.5.4.15 silique
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.15 2'-deoxyguanosine + H2O
-
 Arabidopsis thaliana 2'-deoxyxanthosine + NH3
-
 ?
3.5.4.15 guanosine + H2O
-
 Arabidopsis thaliana xanthosine + NH3
-
 ?
3.5.4.15 additional information no activity with adenine, adenosine, AMP, guanine, GMP, cytosine, cytidine, CMP, cyclic GMP, pterine, and tetrahydrofolate Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.15 ? x * 45000, enzyme-yellow fluorescent protein complex, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.5.4.15 GSDA
-
 Arabidopsis thaliana
3.5.4.15 guanosine deaminase
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.15 0.611
-
 2'-deoxyguanosine at pH 8.0 and 40°C Arabidopsis thaliana
3.5.4.15 1.753
-
 guanosine at pH 8.0 and 40°C Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.4.15 1.061
-
 2'-deoxyguanosine at pH 8.0 and 40°C Arabidopsis thaliana
3.5.4.15 6.642
-
 guanosine at pH 8.0 and 40°C Arabidopsis thaliana