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Literature summary extracted from
- New insight into the catalytic properties of bile salt hydrolase (2013), J. Mol. Catal. B, 96, 46-51.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.24 | DTT | - |
Ligilactobacillus salivarius |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.24 | isozyme BSH1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) , subcloning in Escherichia coli strain JM109 | Ligilactobacillus salivarius |
| 3.5.1.24 | isozyme BSH2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 | Ligilactobacillus salivarius |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.24 | additional information | - |
additional information | isozymes BSH1 and BSH2 are allosteric enzymes, and show positive cooperativity, catalytic efficiency and substrate preference, but with differences, overview. Michaelis-Menten kinetics in the presence of dithiothreitol, but not in its absence. Steady-state kinetics, overview | Ligilactobacillus salivarius | |
| 3.5.1.24 | 0.91 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1, in presence of DTT | Ligilactobacillus salivarius | |
| 3.5.1.24 | 2.17 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2, in presence of DTT | Ligilactobacillus salivarius | |
| 3.5.1.24 | 11.88 | - |
glycodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 13.57 | - |
taurocholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 14.12 | - |
glycodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 14.83 | - |
taurochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 15.02 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 15.04 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 15.05 | - |
glycocholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 15.48 | - |
glycocholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 15.77 | - |
taurodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 15.92 | - |
taurocholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 17.16 | - |
taurochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 18.12 | - |
taurodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 36000 | - |
4 * 36000, recombinant isozyme BSH1, SDS-PAGE | Ligilactobacillus salivarius |
| 3.5.1.24 | 36000 | - |
4 * 36000, recombinant isozyme BSH2, SDS-PAGE | Ligilactobacillus salivarius |
| 3.5.1.24 | 140000 | - |
recombinant isozyme BSH1, gel filtration | Ligilactobacillus salivarius |
| 3.5.1.24 | 142000 | - |
recombinant isozyme BSH2, gel filtration | Ligilactobacillus salivarius |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.24 | Ligilactobacillus salivarius | - |
- |
- |
| 3.5.1.24 | Ligilactobacillus salivarius LMG14476 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.24 | recombinant His-tagged isozyme BSH1 from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography, ultrafiltration, and gel filtration | Ligilactobacillus salivarius |
| 3.5.1.24 | recombinant His-tagged isozyme BSH2 from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography, ultrafiltration, and gel filtration | Ligilactobacillus salivarius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.24 | glycochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | chenodeoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius LMG14476 | chenodeoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycocholic acid + H2O | - |
Ligilactobacillus salivarius | cholate + glycine | - |
? | |
| 3.5.1.24 | glycocholic acid + H2O | - |
Ligilactobacillus salivarius LMG14476 | cholate + glycine | - |
? | |
| 3.5.1.24 | glycodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | deoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius LMG14476 | deoxycholate + glycine | - |
? | |
| 3.5.1.24 | taurochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | chenodeoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius LMG14476 | chenodeoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurocholic acid + H2O | - |
Ligilactobacillus salivarius | cholate + taurine | - |
? | |
| 3.5.1.24 | taurodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | deoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius LMG14476 | deoxycholate + taurine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.24 | homotetramer | 4 * 36000, recombinant isozyme BSH1, SDS-PAGE | Ligilactobacillus salivarius |
| 3.5.1.24 | homotetramer | 4 * 36000, recombinant isozyme BSH2, SDS-PAGE | Ligilactobacillus salivarius |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.24 | bile salt hydrolase | - |
Ligilactobacillus salivarius |
| 3.5.1.24 | BSH1 | - |
Ligilactobacillus salivarius |
| 3.5.1.24 | BSH2 | - |
Ligilactobacillus salivarius |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 37 | - |
assay at | Ligilactobacillus salivarius |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.24 | 33.52 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1, in presence of DTT | Ligilactobacillus salivarius | |
| 3.5.1.24 | 35.32 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2, in presence of DTT | Ligilactobacillus salivarius | |
| 3.5.1.24 | 48.76 | - |
glycodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 53.82 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 70.03 | - |
taurocholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 70.07 | - |
glycocholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 80.87 | - |
glycodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 82.59 | - |
glycocholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 83.15 | - |
taurodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 83.77 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 91.53 | - |
taurochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 93.11 | - |
taurochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 93.14 | - |
taurocholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 99.43 | - |
taurodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 5.5 | 7 | isozyme BSH1, broad optimum | Ligilactobacillus salivarius |
| 3.5.1.24 | 5.5 | 6 | isozyme BSH2 | Ligilactobacillus salivarius |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 5.5 | 7.5 | isozyme BSH1, activity range, profile overview | Ligilactobacillus salivarius |
| 3.5.1.24 | 5.5 | 7.5 | isozyme BSH2, activity range, profile overview | Ligilactobacillus salivarius |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.24 | 3.45 | - |
glycodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 3.58 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 4.53 | - |
glycocholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 5.16 | - |
taurocholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 5.27 | - |
taurodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 5.33 | - |
taurochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 5.49 | - |
glycocholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 5.49 | - |
taurodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 5.57 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 5.85 | - |
taurocholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 6.28 | - |
taurochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 6.81 | - |
glycodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2 | Ligilactobacillus salivarius | |
| 3.5.1.24 | 16.82 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH2, in presence of DTT | Ligilactobacillus salivarius | |
| 3.5.1.24 | 36.84 | - |
glycochenodeoxycholic acid | pH 6.5, 37°C, recombinant isozyme BSH1, in presence of DTT | Ligilactobacillus salivarius | |
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