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Literature summary extracted from
- Identification and characterization of a bile salt hydrolase from Lactobacillus salivarius for development of novel alternatives to antibiotic growth promoters (2012), Appl. Environ. Microbiol., 78, 8795-8802.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.24 | food industry | enzyme inhibitors are promising feed additives to replace antibiotic growth promoters for enhancing the productivity and sustainability of food animals | Ligilactobacillus salivarius |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.24 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) clone (JL 885) | Ligilactobacillus salivarius |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.24 | CaCl2 | 22.4% inhibition at 5 mM | Ligilactobacillus salivarius |  |
| 3.5.1.24 | CoCl2 | 95.9% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | CuCl2 | 98.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | CuSO4 | 91.7% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | FeCl3 | 73% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | FeSO4 | 96.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | KCl | 25.9% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | KI | 36.8% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | KIO3 | 96.5% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | MgCl2 | 25.7% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | MgSO4 | 31.3% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | MnCl2 | 68.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | MnSO4 | 83.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | additional information | no inhibition by citric acid | Ligilactobacillus salivarius | |
| 3.5.1.24 | Na2SeO3 | 93.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | Na2SO4 | 27.7% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | NaCl | 27.7% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | NaHCO3 | 20.6% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | NaI | 31.8% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | NaIO3 | 86.4% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | NaIO4 | 96.4% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | vitamin C | 21.8% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | ZnCl2 | 68.3% inhibition at 5 mM | Ligilactobacillus salivarius | |
| 3.5.1.24 | ZnSO4 | 89.5% inhibition at 5 mM | Ligilactobacillus salivarius | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.24 | 1.15 | - |
glycodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 1.71 | - |
glycocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 2.48 | - |
glycochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 2.5 | 3 | taurochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 3.19 | - |
taurodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 3.21 | - |
taurocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.24 | glycochenodeoxycholic acid + H2O | Ligilactobacillus salivarius | best substrate | chenodeoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycochenodeoxycholic acid + H2O | Ligilactobacillus salivarius NRRL B-30514 | best substrate | chenodeoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycocholic acid + H2O | Ligilactobacillus salivarius | - |
cholate + glycine | - |
? | |
| 3.5.1.24 | glycodeoxycholic acid + H2O | Ligilactobacillus salivarius | - |
deoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycodeoxycholic acid + H2O | Ligilactobacillus salivarius NRRL B-30514 | - |
deoxycholate + glycine | - |
? | |
| 3.5.1.24 | taurochenodeoxycholic acid + H2O | Ligilactobacillus salivarius | - |
chenodeoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurochenodeoxycholic acid + H2O | Ligilactobacillus salivarius NRRL B-30514 | - |
chenodeoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurocholic acid + H2O | Ligilactobacillus salivarius | - |
cholate + taurine | - |
? | |
| 3.5.1.24 | taurodeoxycholic acid + H2O | Ligilactobacillus salivarius | - |
deoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurodeoxycholic acid + H2O | Ligilactobacillus salivarius NRRL B-30514 | - |
deoxycholate + taurine | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.24 | Ligilactobacillus salivarius | J7H3P9 | isolated from chicken | - |
| 3.5.1.24 | Ligilactobacillus salivarius NRRL B-30514 | J7H3P9 | isolated from chicken | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.24 | recombinant expression of His-tagged enzyme from Escherichia coli strain BL21(DE3) clone (JL 885) by nickel affinity chromatography | Ligilactobacillus salivarius |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 4 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate glycodeoxycholic acid | Ligilactobacillus salivarius |
| 3.5.1.24 | 5.6 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate taurocholic acid | Ligilactobacillus salivarius |
| 3.5.1.24 | 7.7 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate glycocholic acid | Ligilactobacillus salivarius |
| 3.5.1.24 | 8 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate taurochenodeoxycholic acid | Ligilactobacillus salivarius |
| 3.5.1.24 | 8.5 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate taurodeoxycholic acid | Ligilactobacillus salivarius |
| 3.5.1.24 | 17.7 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate glycochenodeoxycholic acid | Ligilactobacillus salivarius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.24 | glycochenodeoxycholic acid + H2O | best substrate | Ligilactobacillus salivarius | chenodeoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycochenodeoxycholic acid + H2O | best substrate | Ligilactobacillus salivarius NRRL B-30514 | chenodeoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycocholic acid + H2O | - |
Ligilactobacillus salivarius | cholate + glycine | - |
? | |
| 3.5.1.24 | glycodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | deoxycholate + glycine | - |
? | |
| 3.5.1.24 | glycodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius NRRL B-30514 | deoxycholate + glycine | - |
? | |
| 3.5.1.24 | additional information | broad substrate specificity | Ligilactobacillus salivarius | ? | - |
? | |
| 3.5.1.24 | additional information | broad substrate specificity | Ligilactobacillus salivarius NRRL B-30514 | ? | - |
? | |
| 3.5.1.24 | taurochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | chenodeoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius NRRL B-30514 | chenodeoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurocholic acid + H2O | - |
Ligilactobacillus salivarius | cholate + taurine | - |
? | |
| 3.5.1.24 | taurodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | deoxycholate + taurine | - |
? | |
| 3.5.1.24 | taurodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius NRRL B-30514 | deoxycholate + taurine | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.24 | bile salt hydrolase | - |
Ligilactobacillus salivarius |
| 3.5.1.24 | BSH | - |
Ligilactobacillus salivarius |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 41 | - |
recombinant enzyme | Ligilactobacillus salivarius |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 20 | 55 | activity range, profile overview | Ligilactobacillus salivarius |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.24 | 6.37 | - |
glycodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 8.5 | - |
taurochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 8.87 | - |
glycocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 9.75 | - |
taurocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 13.43 | - |
taurodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 15.6 | - |
glycochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 5.4 | - |
recombinant enzyme | Ligilactobacillus salivarius |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.24 | 4 | 7 | activity range, profile overview | Ligilactobacillus salivarius |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.24 | 3.04 | - |
taurocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 3.36 | - |
taurochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 4.21 | - |
taurodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 5.19 | - |
glycocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 5.54 | - |
glycodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.5.1.24 | 6.29 | - |
glycochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
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