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Literature summary extracted from

  • Ogawa, Y.; Ohnishi, A.; Goto, Y.; Sakuma, Y.; Watanabe, J.; Hattori, A.; Tsujimoto, M.
    Role of glutamine-169 in the substrate recognition of human aminopeptidase B (2014), Biochim. Biophys. Acta, 1840, 1872-1881.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.6 Q169E mutant shows no activity Homo sapiens
3.4.11.6 Q169N mutant shows a significant decrease in hydrolytic activity toward the fluorescent substrate Lys-4-methylcoumaryl-7-amide (MCA). Mutant shows an increase in IC50 values of inhibitors that interact with the catalytic pocket of aminopeptidase B. Km (Arg 4-methylcoumarin 7-amide) similar to wild-type, kcat (Arg 4-methylcoumarin 7-amide) decreased compared to wild-type. Km (Lys 4-methylcoumarin 7-amide) increased compared to wild-type, kcat (Lys 4-methylcoumarin 7-amide) decreased compared to wild-type Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 1,10-phenanthroline
-
 Homo sapiens
3.4.11.6 arphamenine A
-
 Homo sapiens
3.4.11.6 Arphamenine B
-
 Homo sapiens
3.4.11.6 bestatin
-
 Homo sapiens
3.4.11.6 kallidin human peptide hormone, 10 microM, Arg 4-methylcoumarin 7-amide hydrolysis: 73.7% (wild-type enzyme) Homo sapiens
3.4.11.6 Substance P human peptide hormone, 100 microM, Arg 4-methylcoumarin 7-amide hydrolysis: 63.8% (wild-type enzyme); human peptide hormone, 10 microM, Arg 4-methylcoumarin 7-amide hydrolysis: 78% (wild-type enzyme) Homo sapiens
3.4.11.6 ZnCl2
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.6 0.0157
-
 kallidin pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 0.021
-
 Arg-Met-enkephalin pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 0.0251
-
 Met-enkephalin pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 0.0533
-
 Arg 4-methylcoumarin 7-amide pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 0.0651
-
 kallidin pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 0.0774
-
 Arg 4-methylcoumarin 7-amide pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 0.111
-
 Arg-Met-enkephalin pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 0.36
-
 Met-enkephalin pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 0.381
-
 Lys 4-methylcoumarin 7-amide pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 0.568
-
 Lys 4-methylcoumarin 7-amide pH 7.4, 37°C, mutant Q169N Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 Arg 4-methylcoumarin 7-amide + H2O
-
 Homo sapiens Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 Arg-Met-enkephalin + H2O
-
 Homo sapiens ?
-
 ?
3.4.11.6 kallidin + H2O
-
 Homo sapiens ?
-
 ?
3.4.11.6 Lys 4-methylcoumarin 7-amide + H2O
-
 Homo sapiens Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 Met-enkephalin + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.6 aminopeptidase B
-
 Homo sapiens
3.4.11.6 APB
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.6 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.6 0.47
-
 Met-enkephalin pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 0.48
-
 Met-enkephalin pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 1.07
-
 Arg-Met-enkephalin pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 1.95
-
 Lys 4-methylcoumarin 7-amide pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 1.99
-
 kallidin pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 2.35
-
 kallidin pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 3.63
-
 Arg-Met-enkephalin pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 4.06
-
 Arg 4-methylcoumarin 7-amide pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 9.27
-
 Arg 4-methylcoumarin 7-amide pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 14.9
-
 Lys 4-methylcoumarin 7-amide pH 7.4, 37°C, wild-type Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.6 7.4
-
 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.11.6 0.00000072
-
 pH 7.4, 37°C, wild-type Homo sapiens Arphamenine B
3.4.11.6 0.00000155
-
 pH 7.4, 37°C, mutant Q169N Homo sapiens Arphamenine B
3.4.11.6 0.00000316
-
 pH 7.4, 37°C, mutant Q169N Homo sapiens arphamenine A
3.4.11.6 0.00000349
-
 pH 7.4, 37°C, wild-type Homo sapiens bestatin
3.4.11.6 0.00000373
-
 pH 7.4, 37°C, wild-type Homo sapiens arphamenine A
3.4.11.6 0.00000391
-
 pH 7.4, 37°C, wild-type Homo sapiens ZnCl2
3.4.11.6 0.00000592
-
 pH 7.4, 37°C, mutant Q169N Homo sapiens ZnCl2
3.4.11.6 0.00006
-
 pH 7.4, 37°C, mutant Q169N Homo sapiens 1,10-phenanthroline
3.4.11.6 0.000077
-
 pH 7.4, 37°C, wild-type Homo sapiens 1,10-phenanthroline
3.4.11.6 0.000289
-
 pH 7.4, 37°C, mutant Q169N Homo sapiens bestatin

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.6 1
-
 Met-enkephalin pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 10.4
-
 Arg-Met-enkephalin pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 19
-
 Met-enkephalin pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 30.1
-
 kallidin pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 156
-
 kallidin pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 173
-
 Arg-Met-enkephalin pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 3440
-
 Lys 4-methylcoumarin 7-amide pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 39200
-
 Lys 4-methylcoumarin 7-amide pH 7.4, 37°C, wild-type Homo sapiens
3.4.11.6 52400
-
 Arg 4-methylcoumarin 7-amide pH 7.4, 37°C, mutant Q169N Homo sapiens
3.4.11.6 175000
-
 Arg 4-methylcoumarin 7-amide pH 7.4, 37°C, wild-type Homo sapiens