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Literature summary extracted from

  • Joo, Y.C.; Seo, E.S.; Kim, Y.S.; Kim, K.R.; Park, J.B.; Oh, D.K.
    Production of 10-hydroxystearic acid from oleic acid by whole cells of recombinant Escherichia coli containing oleate hydratase from Stenotrophomonas maltophilia (2012), J. Biotechnol., 158, 17-23.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.53 Tween 40 maximum activity at 0.05% (v/v) Tween 40 Stenotrophomonas maltophilia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.53 expressed in Escherichia coli ER2566, BL21(DE3), W3110, and MLK1067 cells Stenotrophomonas maltophilia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.53 0.46
-
 oleate at pH 6.5 and 35°C Stenotrophomonas maltophilia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.53 68000
-
 2 * 68000, SDS-PAGE Stenotrophomonas maltophilia
4.2.1.53 136000
-
 gel filtration Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.53 oleate + H2O Stenotrophomonas maltophilia highest specific activity (R)-10-hydroxystearate 98% yield after 4 h ?
4.2.1.53 oleate + H2O Stenotrophomonas maltophilia KCTC 1773 highest specific activity (R)-10-hydroxystearate 98% yield after 4 h ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.53 Stenotrophomonas maltophilia
-
-
-
4.2.1.53 Stenotrophomonas maltophilia KCTC 1773
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.53 His-Trap column chromatography Stenotrophomonas maltophilia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.53 0.014
-
 with myristoleic acid as substrate, at pH 6.5 and 35°C Stenotrophomonas maltophilia
4.2.1.53 0.033
-
 with gamma-linolenic acid as substrate, at pH 6.5 and 35°C Stenotrophomonas maltophilia
4.2.1.53 0.54
-
 with alpha-linolenic acid as substrate, at pH 6.5 and 35°C Stenotrophomonas maltophilia
4.2.1.53 0.91
-
 with linoleic acid as substrate, at pH 6.5 and 35°C Stenotrophomonas maltophilia
4.2.1.53 1.95
-
 with palmitoleic acid as substrate, at pH 6.5 and 35°C Stenotrophomonas maltophilia
4.2.1.53 3.69
-
 with oleate as substrate, at pH 6.5 and 35°C Stenotrophomonas maltophilia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.53 alpha-linolenic acid + H2O
-
 Stenotrophomonas maltophilia 10-hydroxy-12,15(Z,Z)-octadecadienoic acid
-
 ?
4.2.1.53 alpha-linolenic acid + H2O
-
 Stenotrophomonas maltophilia KCTC 1773 10-hydroxy-12,15(Z,Z)-octadecadienoic acid
-
 ?
4.2.1.53 gamma-linolenic acid + H2O
-
 Stenotrophomonas maltophilia 10-hydroxy-6,12(Z,Z)-octadecadienoic acid
-
 ?
4.2.1.53 gamma-linolenic acid + H2O
-
 Stenotrophomonas maltophilia KCTC 1773 10-hydroxy-6,12(Z,Z)-octadecadienoic acid
-
 ?
4.2.1.53 linoleic acid + H2O
-
 Stenotrophomonas maltophilia 10-hydroxy-12(Z)-octadecenoic acid
-
 ?
4.2.1.53 additional information no hydration activity for myristic acid, palmitic acid, stearic acid, arachidic acid, petroselinic acid, elaidic acid, vaccenic acid, conjugated linoleic acids, arachidonic acid, erucic ac, and nervonic acid Stenotrophomonas maltophilia ?
-
 ?
4.2.1.53 additional information no hydration activity for myristic acid, palmitic acid, stearic acid, arachidic acid, petroselinic acid, elaidic acid, vaccenic acid, conjugated linoleic acids, arachidonic acid, erucic ac, and nervonic acid Stenotrophomonas maltophilia KCTC 1773 ?
-
 ?
4.2.1.53 myristoleic acid + H2O
-
 Stenotrophomonas maltophilia 10-hydroxymyristic acid
-
 ?
4.2.1.53 oleate + H2O highest specific activity Stenotrophomonas maltophilia (R)-10-hydroxystearate 98% yield after 4 h ?
4.2.1.53 oleate + H2O highest specific activity Stenotrophomonas maltophilia KCTC 1773 (R)-10-hydroxystearate 98% yield after 4 h ?
4.2.1.53 Palmitoleic acid + H2O
-
 Stenotrophomonas maltophilia 10-Hydroxypalmitic acid
-
 ?
4.2.1.53 Palmitoleic acid + H2O
-
 Stenotrophomonas maltophilia KCTC 1773 10-Hydroxypalmitic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.53 homodimer 2 * 68000, SDS-PAGE Stenotrophomonas maltophilia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.53 35
-
-
 Stenotrophomonas maltophilia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.53 11.83
-
 oleate at pH 6.5 and 35°C Stenotrophomonas maltophilia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.53 6.5
-
-
 Stenotrophomonas maltophilia

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.53 additional information the addition of FAD (0.01-0.2 mM) has no effect on the activity Stenotrophomonas maltophilia

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.53 25.67
-
 oleate at pH 6.5 and 35°C Stenotrophomonas maltophilia