Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, B.N.; Joo, Y.C.; Kim, Y.S.; Kim, K.R.; Oh, D.K.
    Production of 10-hydroxystearic acid from oleic acid and olive oil hydrolyzate by an oleate hydratase from Lysinibacillus fusiformis (2012), Appl. Microbiol. Biotechnol., 95, 929-937.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.53 ethanol 120% activity at 4% (v/v) ethanol Lysinibacillus fusiformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.53 expressed in Escherichia coli ER2566 cells Lysinibacillus fusiformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.53 ethanol less than 60% residual activity at 6% (v/v) ethanol Lysinibacillus fusiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.53 0.54
-
 oleate at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 0.58
-
 linoleate at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 0.76
-
 palmitoleate at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 0.77
-
 gamma-linolenate at pH 6.5 and 35°C Lysinibacillus fusiformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.53 69000
-
 2 * 69000, SDS-PAGE Lysinibacillus fusiformis
4.2.1.53 69200
-
 2 * 69200, calculated from amino acid sequence Lysinibacillus fusiformis
4.2.1.53 138000
-
 gel filtration Lysinibacillus fusiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.53 oleate + H2O Lysinibacillus fusiformis highest activity (R)-10-hydroxystearate
-
 ?
4.2.1.53 oleate + H2O Lysinibacillus fusiformis KCTC 3454 highest activity (R)-10-hydroxystearate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.53 Lysinibacillus fusiformis
-
-
-
4.2.1.53 Lysinibacillus fusiformis KCTC 3454
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.53 His-Trap column chromatography Lysinibacillus fusiformis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.53 20
-
 with alpha-linolenate as substrate, at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 30
-
 with myristoleate as substrate, at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 110
-
 with linoleate as substrate, at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 160
-
 with gamma-linolenate as substrate, at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 550
-
 with palmitoleate as substrate, at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 1580
-
 with oleate as substrate, at pH 6.5 and 35°C Lysinibacillus fusiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.53 alpha-linolenate + H2O
-
 Lysinibacillus fusiformis (R)-10-hydroxy-12,15(Z,Z)-octadecadienoate
-
 ?
4.2.1.53 alpha-linolenate + H2O
-
 Lysinibacillus fusiformis KCTC 3454 (R)-10-hydroxy-12,15(Z,Z)-octadecadienoate
-
 ?
4.2.1.53 gamma-linolenate + H2O
-
 Lysinibacillus fusiformis (R)-10-hydroxy-6,12-(Z,Z)-octadecadienoate
-
 ?
4.2.1.53 gamma-linolenate + H2O
-
 Lysinibacillus fusiformis KCTC 3454 (R)-10-hydroxy-6,12-(Z,Z)-octadecadienoate
-
 ?
4.2.1.53 linoleate + H2O
-
 Lysinibacillus fusiformis (R)-10-hydroxy-12(Z)-octadecenoate
-
 ?
4.2.1.53 linoleate + H2O
-
 Lysinibacillus fusiformis KCTC 3454 (R)-10-hydroxy-12(Z)-octadecenoate
-
 ?
4.2.1.53 myristoleate + H2O
-
 Lysinibacillus fusiformis (R)-10-hydroxymyristate
-
 ?
4.2.1.53 myristoleate + H2O
-
 Lysinibacillus fusiformis KCTC 3454 (R)-10-hydroxymyristate
-
 ?
4.2.1.53 oleate + H2O highest activity Lysinibacillus fusiformis (R)-10-hydroxystearate
-
 ?
4.2.1.53 oleate + H2O highest activity Lysinibacillus fusiformis KCTC 3454 (R)-10-hydroxystearate
-
 ?
4.2.1.53 palmitoleate + H2O
-
 Lysinibacillus fusiformis (R)-10-hydroxypalmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.53 homodimer 2 * 69000, SDS-PAGE Lysinibacillus fusiformis
4.2.1.53 homodimer 2 * 69200, calculated from amino acid sequence Lysinibacillus fusiformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.53 35
-
-
 Lysinibacillus fusiformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.53 20 45 more than 60% activity between 20 and 45°C Lysinibacillus fusiformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.53 1.33
-
 linoleate at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 2.17
-
 gamma-linolenate at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 7.17
-
 palmitoleate at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 14.17
-
 oleate at pH 6.5 and 35°C Lysinibacillus fusiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.53 6.5
-
-
 Lysinibacillus fusiformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.53 5.5 7.5 more than 50% activity between pH 5.5 and 7.5 Lysinibacillus fusiformis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.53 2.33
-
 linoleate at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 2.67
-
 gamma-linolenate at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 9.33
-
 palmitoleate at pH 6.5 and 35°C Lysinibacillus fusiformis
4.2.1.53 26.17
-
 oleate at pH 6.5 and 35°C Lysinibacillus fusiformis