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Literature summary extracted from

  • Elkin, S.R.; Kumar, A.; Price, C.W.; Columbus, L.
    A broad specificity nucleoside kinase from Thermoplasma acidophilum (2013), Proteins, 81, 568-582.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.B20
-
 Thermoplasma acidophilum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.B20 sitting nanodroplet vapor diffusion method, crystal structure of Ta0880, determined at 1.91 A resolution reveals a dimer with each monomer composed of an alpha/beta/alpha sandwich domain and a smaller lid domain Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.B20 0.000208
-
 guanosine pH 8.0, 22°C Thermoplasma acidophilum
2.7.1.B20 0.000411
-
 cytidine pH 8.0, 22°C Thermoplasma acidophilum
2.7.1.B20 0.000712
-
 Inosine pH 8.0, 22°C Thermoplasma acidophilum
2.7.1.B20 0.001
-
 adenosine pH 8.0, 22°C Thermoplasma acidophilum
2.7.1.B20 0.0469
-
 ATP pH 8.0, 22°C, cosubstrate: inosine Thermoplasma acidophilum
2.7.1.B20 0.185
-
 GTP pH 8.0, 22°C Thermoplasma acidophilum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.B20 intracellular
-
 Thermoplasma acidophilum 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.B20 Co2+ kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+ Thermoplasma acidophilum
2.7.1.B20 Mg2+ kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+ Thermoplasma acidophilum
2.7.1.B20 Mn2+ kinase activity is observed with Mg2+ and Co2+ and to a lesser degree with Mn2+ Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.B20 32804
-
 2 * 32804, calculated from sequence Thermoplasma acidophilum
2.7.1.B20 33000
-
 2 * 33000, SDS-PAGE, crystal structure Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.B20 Thermoplasma acidophilum Q9HJT3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.B20
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.B20 ATP + adenosine catalytic efficiency with cytidine is 22% relative to guanosine Thermoplasma acidophilum ADP + adenosine 5'-phosphate
-
 ?
2.7.1.B20 ATP + cytidine catalytic efficiency with cytidine is 45% relative to guanosine Thermoplasma acidophilum ADP + cytidine 5'-phosphate
-
 ?
2.7.1.B20 ATP + guanosine phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP. The catalytic efficiency of GTP is 39% compared to that of ATP Thermoplasma acidophilum ADP + guanosine 5'-phosphate
-
 ?
2.7.1.B20 ATP + inosine catalytic efficiency with inosine is 62% relative to guanosine Thermoplasma acidophilum ADP + inosine 5'-phosphate
-
 ?
2.7.1.B20 GTP + guanosine phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP. The catalytic efficiency of GTP is 39% compared to that of ATP Thermoplasma acidophilum GDP + guanosine 5'-phosphate
-
 ?
2.7.1.B20 ITP + guanosine phosphoryl donor specificity in order of decreasing Vmax: GTP > ATP > ITP Thermoplasma acidophilum IDP + guanosine 5'-phosphate
-
 ?
2.7.1.B20 additional information negligible enzymatic activity with thymidine, uridine, and 2-deoxyadenosine. Negligible enzymatic activity is observed with UTP and TTP as phosphate donors Thermoplasma acidophilum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.B20 dimer 2 * 32804, calculated from sequence Thermoplasma acidophilum
2.7.1.B20 dimer 2 * 33000, SDS-PAGE, crystal structure Thermoplasma acidophilum

Synonyms

EC Number Synonyms Comment Organism
2.7.1.B20 Ta0880 locus name Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.B20 22
-
 assay at Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.B20 0.064
-
 cytidine pH 8.0, 22°C Thermoplasma acidophilum
2.7.1.B20 0.072
-
 guanosine pH 8.0, 22°C Thermoplasma acidophilum
2.7.1.B20 0.084
-
 adenosine pH 8.0, 22°C Thermoplasma acidophilum
2.7.1.B20 0.16
-
 Inosine pH 8.0, 22°C Thermoplasma acidophilum
2.7.1.B20 0.17
-
 ATP pH 8.0, 22°C, cosubstrate: inosine Thermoplasma acidophilum
2.7.1.B20 0.27
-
 GTP pH 8.0, 22°C Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.B20 6.6
-
-
 Thermoplasma acidophilum
2.7.1.B20 8
-
 assay at Thermoplasma acidophilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.B20 6.2 7.8 pH 6.2: about 60% of maximal activity, pH 7.8: about 75% of maximal activity Thermoplasma acidophilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.B20 1.4
-
 GTP pH 8.0, 22°C Thermoplasma acidophilum
2.7.1.B20 3.7
-
 ATP pH 8.0, 22°C, cosubstrate: inosine Thermoplasma acidophilum
2.7.1.B20 74.9
-
 adenosine pH 8.0, 22°C Thermoplasma acidophilum
2.7.1.B20 156
-
 cytidine pH 8.0, 22°C Thermoplasma acidophilum
2.7.1.B20 215
-
 Inosine pH 8.0, 22°C Thermoplasma acidophilum
2.7.1.B20 345
-
 guanosine pH 8.0, 22°C Thermoplasma acidophilum