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Literature summary extracted from

  • Dadashipour, M.; Yamazaki, M.; Momonoi, K.; Tamura, K.; Fuhshuku, K.; Kanase, Y.; Uchimura, E.; Kaiyun, G.; Asano, Y.
    S-selective hydroxynitrile lyase from a plant Baliospermum montanum: molecular characterization of recombinant enzyme (2011), J. Biotechnol., 153, 100-110.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.47 expression in Escherichia coli Baliospermum montanum

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.47 D207A mutant protein is insoluble Baliospermum montanum
4.1.2.47 H235A mutant protein is insoluble Baliospermum montanum
4.1.2.47 additional information C-terminally truncated enzymes exhibit activities of 75, 102, 65 and 48 U/l for enzyme after removing 2, 4, 6, and 8 amino acids, respectively Baliospermum montanum
4.1.2.47 S80A mutant protein is insoluble Baliospermum montanum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.47 Ag+ 1 mM, 12% residual activity Baliospermum montanum
4.1.2.47 methyl ethyl ketone 50 mM, 27% residual activity Baliospermum montanum
4.1.2.47 phenol 50 mM, 32% residual activity Baliospermum montanum
4.1.2.47 Phenylmethyl sulfonylfluoride 1 mM, 27% residual activity Baliospermum montanum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.47 5.2
-
 2,4-dimethylbenzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 5.5
-
 benzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 9.8
-
 3-Methoxybenzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 14.4
-
 3-methylbenzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 15.7
-
 piperonal pH 6.0, 25°C Baliospermum montanum
4.1.2.47 21.7
-
 2-Methylbenzaldehyde pH 6.0, 25°C Baliospermum montanum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.47 29500
-
 2 * 29500, SDS-PAGE Baliospermum montanum
4.1.2.47 60000 65000 gel filtration Baliospermum montanum

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.47 Baliospermum montanum D1MX73
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.47 leaf
-
 Baliospermum montanum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.47 178
-
 pH 6.0, 25°C Baliospermum montanum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.47 cyanide + 2,4-dimethylbenzaldehyde
-
 Baliospermum montanum (2S)-(2,4-dimethylphenyl)(hydroxy)ethanenitrile 65% yield, 82% enantiomeric excess ?
4.1.2.47 cyanide + 2-methylbenzaldehyde
-
 Baliospermum montanum (2S)-(2-methylphenyl)(hydroxy)ethanenitrile 76% yield, 47% enantiomeric excess ?
4.1.2.47 cyanide + 3-methoxybenzaldehyde
-
 Baliospermum montanum (2S)-(3-methoxyphenyl)(hydroxy)ethanenitrile 67% yield, 76% enantiomeric excess ?
4.1.2.47 cyanide + 3-methylbenzaldehyde
-
 Baliospermum montanum (2S)-(3-methylphenyl)(hydroxy)ethanenitrile 76% yield, 76% enantiomeric excess ?
4.1.2.47 cyanide + benzaldehyde
-
 Baliospermum montanum (S)-mandelonitrile 68% yield, 54% enantiomeric excess ?
4.1.2.47 cyanide + piperonal
-
 Baliospermum montanum ? 47% yield, 74% enantiomeric excess ?

Subunits

EC Number Subunits Comment Organism
4.1.2.47 dimer 2 * 29500, SDS-PAGE Baliospermum montanum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.47 20
-
-
 Baliospermum montanum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.47
-
 65
-
 Baliospermum montanum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.47 18.5
-
 2,4-dimethylbenzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 26.6
-
 benzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 28.1
-
 3-Methoxybenzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 49.2
-
 3-methylbenzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 53.6
-
 2-Methylbenzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 91.4
-
 piperonal pH 6.0, 25°C Baliospermum montanum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.47 5
-
-
 Baliospermum montanum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.47 4 6.5
-
 Baliospermum montanum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.47 2.4 11
-
 Baliospermum montanum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.47 2.47
-
 2-Methylbenzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 2.87
-
 3-Methoxybenzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 3.41
-
 3-methylbenzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 3.56
-
 2,4-dimethylbenzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 4.8
-
 benzaldehyde pH 6.0, 25°C Baliospermum montanum
4.1.2.47 5.82
-
 piperonal pH 6.0, 25°C Baliospermum montanum