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Literature summary extracted from

  • Tural, B.; Tural, S.; Demir, A.S.
    Carboligation reactions mediated by benzoylformate decarboxylase immobilized on a magnetic solid support (2013), Chirality, 25, 415-421.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.7 synthesis purified recombinant His-tagged enzyme immobilized on magnetic nanoparticles, microspheres, nanospheres and ferrofluids, is used for enantioselective synthesis of (S)-2-hydroxyketones. Its stereoselectivity is highly dependent on the structure of the substrate aldehydes. The heterogeneous biocatalyst is offering the ease of immobilization along with the separation steps of metal affinity ligand (Cu2+-iminodiacetic acid) coated magnetic nanoparticles. Reusability of immobilized enzyme for carboligation reactions Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.7 expression of His-tagged enzyme in Escherichia coli strain SG13009 Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.7 additional information immobilization of the purified recombinant His-tagged enzyme on magnetic nanoparticles, microspheres, nanospheres and ferrofluids, for enantioselective synthesis of (S)-2-hydroxyketones. Its stereoselectivity is highly dependent on the structure of the substrate aldehydes. Adsorption capacity of the Cu+2 charged magnetic solid support for His-tagged BFD is determined by standard BSA assay as 43.6 mg enzyme/g magnetic solid support Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.7 56000
-
 x * 56000, recombinant His-tagged enzyme, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.7 additional information Pseudomonas putida the enzyme performs enantioselective synthesis of (S)-2-hydroxyketones. Its stereoselectivity is highly dependent on the structure of the substrate aldehydes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 2 benzaldehyde
-
 Pseudomonas putida (R)-benzoin i.e. (R)-2-hydroxy-1,2-diphenylethan-1-one ?
4.1.1.7 additional information the enzyme performs enantioselective synthesis of (S)-2-hydroxyketones. Its stereoselectivity is highly dependent on the structure of the substrate aldehydes Pseudomonas putida ?
-
 ?
4.1.1.7 additional information the enzyme also performs the cross acyloin reaction of benzaldehyde with acetaldehyde to give (S)-2-hydroxy-1-phenylpropanone Pseudomonas putida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.7 ? x * 56000, recombinant His-tagged enzyme, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
4.1.1.7 BFD
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.7 30
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.7 7
-
 assay at Pseudomonas putida

General Information

EC Number General Information Comment Organism
4.1.1.7 physiological function the enzyme functions in decarboxylation of benzoylformate to benzaldehyde in the mandalate pathway Pseudomonas putida