EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.18.1 | expressed in Escherichia coli BL21(DE3) cells | Ralstonia solanacearum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.18.1 | 145-D317Y | the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine | Ralstonia solanacearum |
1.14.18.1 | 145-L330V | the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine | Ralstonia solanacearum |
1.14.18.1 | 145-V153A | the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine | Ralstonia solanacearum |
1.14.18.1 | 145-Y119F | the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine | Ralstonia solanacearum |
1.14.18.1 | C10-F185Y | the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine | Ralstonia solanacearum |
1.14.18.1 | C10-N322S | the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine | Ralstonia solanacearum |
1.14.18.1 | C10-T183I | the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine | Ralstonia solanacearum |
1.14.18.1 | C10-T359M | the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine | Ralstonia solanacearum |
1.14.18.1 | RV145 | the random mutation variant exhibits a 3.2fold increase in kcat, respectively, compared to the wild type while the Km is 5.2fold lower. The overall catalytic efficiency for the variant has improved 16fold compared to the wild type for D-tyrosine | Ralstonia solanacearum |
1.14.18.1 | RVC10 | the random mutation variant exhibits a 2.1fold increase in kcat, respectively, compared to the wild type while the Km is 3.2fold lower. The overall catalytic efficiency for the variant has improved 6.7fold compared to the wild type for D-tyrosine | Ralstonia solanacearum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.18.1 | 0.6 | - |
L-tyrosine | random mutant enzyme RV145, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.9 | - |
L-tyrosine | random mutant enzyme RVC10, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1 | - |
L-tyrosine | random mutant enzyme C10-N322S, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.2 | - |
D-tyrosine | random mutant enzyme RV145, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.9 | - |
D-tyrosine | random mutant enzyme RVC10, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 2.1 | - |
L-tyrosine | wild type enzyme, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 2.4 | - |
D-tyrosine | random mutant enzyme C10-N322S, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 3.5 | - |
D-tyrosine | random mutant enzyme C10-T183I, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 5.1 | - |
D-tyrosine | random mutant enzyme 145-Y119F, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 6.2 | - |
D-tyrosine | wild type enzyme, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 6.3 | - |
D-tyrosine | random mutant enzyme C10-T359M, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 6.9 | - |
D-tyrosine | random mutant enzyme 145-D317Y, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 7.3 | - |
D-tyrosine | random mutant enzyme C10-F185Y, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 9.6 | - |
D-tyrosine | random mutant enzyme 145-L330V, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 15 | - |
D-tyrosine | random mutant enzyme 145-V153A, at pH 7.0 and 30°C | Ralstonia solanacearum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 55000 | - |
x * 55000, SDS-PAGE | Ralstonia solanacearum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | D-tyrosine + O2 | Ralstonia solanacearum | - |
dopaquinone + H2O | - |
? | |
1.14.18.1 | L-tyrosine + O2 | Ralstonia solanacearum | - |
dopaquinone + H2O | - |
? | |
1.14.18.1 | monophenol + O2 | Ralstonia solanacearum | - |
o-diphenol + H2O | - |
? | |
1.14.18.1 | o-diphenol + O2 | Ralstonia solanacearum | - |
o-quinone + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.18.1 | Ralstonia solanacearum | Q8Y2J8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.18.1 | - |
Ralstonia solanacearum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | D-tyrosine + O2 | - |
Ralstonia solanacearum | dopaquinone + H2O | - |
? | |
1.14.18.1 | L-tyrosine + O2 | - |
Ralstonia solanacearum | dopaquinone + H2O | - |
? | |
1.14.18.1 | monophenol + O2 | - |
Ralstonia solanacearum | o-diphenol + H2O | - |
? | |
1.14.18.1 | o-diphenol + O2 | - |
Ralstonia solanacearum | o-quinone + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.18.1 | ? | x * 55000, SDS-PAGE | Ralstonia solanacearum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.18.1 | catecholase | - |
Ralstonia solanacearum |
1.14.18.1 | monophenol monooxygenase | - |
Ralstonia solanacearum |
1.14.18.1 | monophenol, L-DOPA: oxygen oxidoreductase | - |
Ralstonia solanacearum |
1.14.18.1 | polyphenol oxidase | - |
Ralstonia solanacearum |
1.14.18.1 | PPO | - |
Ralstonia solanacearum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.18.1 | 0.3 | - |
D-tyrosine | wild type enzyme, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.63 | - |
D-tyrosine | random mutant enzyme RVC10, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.92 | - |
L-tyrosine | random mutant enzyme RV145, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.97 | - |
D-tyrosine | random mutant enzyme RV145, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.03 | - |
L-tyrosine | random mutant enzyme RVC10, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.22 | - |
D-tyrosine | random mutant enzyme C10-N322S, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.24 | - |
L-tyrosine | random mutant enzyme C10-N322S, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.27 | - |
D-tyrosine | random mutant enzyme 145-Y119F, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.35 | - |
D-tyrosine | random mutant enzyme 145-D317Y, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.37 | - |
D-tyrosine | random mutant enzyme C10-T183I, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.37 | - |
D-tyrosine | random mutant enzyme C10-T359M, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.42 | - |
L-tyrosine | wild type enzyme, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.63 | - |
D-tyrosine | random mutant enzyme 145-L330V, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.7 | - |
D-tyrosine | random mutant enzyme C10-F185Y, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 2.1 | - |
D-tyrosine | random mutant enzyme 145-V153A, at pH 7.0 and 30°C | Ralstonia solanacearum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | heme | - |
Ralstonia solanacearum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.18.1 | 0.05 | - |
D-tyrosine | wild type enzyme, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.14 | - |
D-tyrosine | random mutant enzyme 145-V153A, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.17 | - |
D-tyrosine | random mutant enzyme 145-L330V, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.2 | - |
D-tyrosine | random mutant enzyme 145-D317Y, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.22 | - |
D-tyrosine | random mutant enzyme C10-T359M, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.23 | - |
D-tyrosine | random mutant enzyme C10-F185Y, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.25 | - |
D-tyrosine | random mutant enzyme 145-Y119F, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.33 | - |
D-tyrosine | random mutant enzyme RVC10, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.39 | - |
D-tyrosine | random mutant enzyme C10-T183I, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.51 | - |
D-tyrosine | random mutant enzyme C10-N322S, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.67 | - |
L-tyrosine | wild type enzyme, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 0.8 | - |
D-tyrosine | random mutant enzyme RV145, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.12 | - |
L-tyrosine | random mutant enzyme RVC10, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.24 | - |
L-tyrosine | random mutant enzyme C10-N322S, at pH 7.0 and 30°C | Ralstonia solanacearum | |
1.14.18.1 | 1.42 | - |
L-tyrosine | random mutant enzyme RV145, at pH 7.0 and 30°C | Ralstonia solanacearum |