Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Molloy, S.; Nikodinovic-Runic, J.; Martin, L.B.; Hartmann, H.; Solano, F.; Decker, H.; OConnor, K.E.
    Engineering of a bacterial tyrosinase for improved catalytic efficiency towards D-tyrosine using random and site directed mutagenesis approaches (2013), Biotechnol. Bioeng., 110, 1849-1857.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.1 expressed in Escherichia coli BL21(DE3) cells Ralstonia solanacearum

Protein Variants

EC Number Protein Variants Comment Organism
1.14.18.1 145-D317Y the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine Ralstonia solanacearum
1.14.18.1 145-L330V the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine Ralstonia solanacearum
1.14.18.1 145-V153A the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine Ralstonia solanacearum
1.14.18.1 145-Y119F the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine Ralstonia solanacearum
1.14.18.1 C10-F185Y the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine Ralstonia solanacearum
1.14.18.1 C10-N322S the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine Ralstonia solanacearum
1.14.18.1 C10-T183I the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine Ralstonia solanacearum
1.14.18.1 C10-T359M the overall catalytic efficiency for the variant has improved compared to the wild type for D-tyrosine Ralstonia solanacearum
1.14.18.1 RV145 the random mutation variant exhibits a 3.2fold increase in kcat, respectively, compared to the wild type while the Km is 5.2fold lower. The overall catalytic efficiency for the variant has improved 16fold compared to the wild type for D-tyrosine Ralstonia solanacearum
1.14.18.1 RVC10 the random mutation variant exhibits a 2.1fold increase in kcat, respectively, compared to the wild type while the Km is 3.2fold lower. The overall catalytic efficiency for the variant has improved 6.7fold compared to the wild type for D-tyrosine Ralstonia solanacearum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.6
-
L-tyrosine random mutant enzyme RV145, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.9
-
L-tyrosine random mutant enzyme RVC10, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1
-
L-tyrosine random mutant enzyme C10-N322S, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.2
-
D-tyrosine random mutant enzyme RV145, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.9
-
D-tyrosine random mutant enzyme RVC10, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 2.1
-
L-tyrosine wild type enzyme, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 2.4
-
D-tyrosine random mutant enzyme C10-N322S, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 3.5
-
D-tyrosine random mutant enzyme C10-T183I, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 5.1
-
D-tyrosine random mutant enzyme 145-Y119F, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 6.2
-
D-tyrosine wild type enzyme, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 6.3
-
D-tyrosine random mutant enzyme C10-T359M, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 6.9
-
D-tyrosine random mutant enzyme 145-D317Y, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 7.3
-
D-tyrosine random mutant enzyme C10-F185Y, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 9.6
-
D-tyrosine random mutant enzyme 145-L330V, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 15
-
D-tyrosine random mutant enzyme 145-V153A, at pH 7.0 and 30°C Ralstonia solanacearum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 55000
-
x * 55000, SDS-PAGE Ralstonia solanacearum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.1 D-tyrosine + O2 Ralstonia solanacearum
-
dopaquinone + H2O
-
?
1.14.18.1 L-tyrosine + O2 Ralstonia solanacearum
-
dopaquinone + H2O
-
?
1.14.18.1 monophenol + O2 Ralstonia solanacearum
-
o-diphenol + H2O
-
?
1.14.18.1 o-diphenol + O2 Ralstonia solanacearum
-
o-quinone + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Ralstonia solanacearum Q8Y2J8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1
-
Ralstonia solanacearum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 D-tyrosine + O2
-
Ralstonia solanacearum dopaquinone + H2O
-
?
1.14.18.1 L-tyrosine + O2
-
Ralstonia solanacearum dopaquinone + H2O
-
?
1.14.18.1 monophenol + O2
-
Ralstonia solanacearum o-diphenol + H2O
-
?
1.14.18.1 o-diphenol + O2
-
Ralstonia solanacearum o-quinone + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 ? x * 55000, SDS-PAGE Ralstonia solanacearum

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 catecholase
-
Ralstonia solanacearum
1.14.18.1 monophenol monooxygenase
-
Ralstonia solanacearum
1.14.18.1 monophenol, L-DOPA: oxygen oxidoreductase
-
Ralstonia solanacearum
1.14.18.1 polyphenol oxidase
-
Ralstonia solanacearum
1.14.18.1 PPO
-
Ralstonia solanacearum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.18.1 0.3
-
D-tyrosine wild type enzyme, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.63
-
D-tyrosine random mutant enzyme RVC10, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.92
-
L-tyrosine random mutant enzyme RV145, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.97
-
D-tyrosine random mutant enzyme RV145, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.03
-
L-tyrosine random mutant enzyme RVC10, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.22
-
D-tyrosine random mutant enzyme C10-N322S, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.24
-
L-tyrosine random mutant enzyme C10-N322S, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.27
-
D-tyrosine random mutant enzyme 145-Y119F, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.35
-
D-tyrosine random mutant enzyme 145-D317Y, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.37
-
D-tyrosine random mutant enzyme C10-T183I, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.37
-
D-tyrosine random mutant enzyme C10-T359M, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.42
-
L-tyrosine wild type enzyme, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.63
-
D-tyrosine random mutant enzyme 145-L330V, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.7
-
D-tyrosine random mutant enzyme C10-F185Y, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 2.1
-
D-tyrosine random mutant enzyme 145-V153A, at pH 7.0 and 30°C Ralstonia solanacearum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.1 heme
-
Ralstonia solanacearum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.18.1 0.05
-
D-tyrosine wild type enzyme, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.14
-
D-tyrosine random mutant enzyme 145-V153A, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.17
-
D-tyrosine random mutant enzyme 145-L330V, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.2
-
D-tyrosine random mutant enzyme 145-D317Y, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.22
-
D-tyrosine random mutant enzyme C10-T359M, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.23
-
D-tyrosine random mutant enzyme C10-F185Y, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.25
-
D-tyrosine random mutant enzyme 145-Y119F, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.33
-
D-tyrosine random mutant enzyme RVC10, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.39
-
D-tyrosine random mutant enzyme C10-T183I, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.51
-
D-tyrosine random mutant enzyme C10-N322S, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.67
-
L-tyrosine wild type enzyme, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 0.8
-
D-tyrosine random mutant enzyme RV145, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.12
-
L-tyrosine random mutant enzyme RVC10, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.24
-
L-tyrosine random mutant enzyme C10-N322S, at pH 7.0 and 30°C Ralstonia solanacearum
1.14.18.1 1.42
-
L-tyrosine random mutant enzyme RV145, at pH 7.0 and 30°C Ralstonia solanacearum