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Literature summary extracted from

  • Steeves, C.H.; Bearne, S.L.
    Activation and inhibition of CTP synthase from Trypanosoma brucei, the causative agent of African sleeping sickness (2011), Bioorg. Med. Chem. Lett., 21, 5188-5190.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.2 GTP activates glutamine-dependent CTP formation at concentrations below 0.2 mM Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.2 expressed in Escherichia coli BL21(DE3) cells Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.2 Caffeine
-
 Trypanosoma brucei
6.3.4.2 GTP inhibits glutamine-dependent CTP formation at concentrations above 0.2 mM Trypanosoma brucei
6.3.4.2 guanosine
-
 Trypanosoma brucei
6.3.4.2 uric acid
-
 Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.2 70000
-
 x * 70000, SDS-PAGE Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.2 ATP + UTP + L-glutamine Trypanosoma brucei
-
 ADP + phosphate + CTP + L-glutamate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.2 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.2 Ni2+-IDA resin column chromatography Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.2 ATP + UTP + L-glutamine
-
 Trypanosoma brucei ADP + phosphate + CTP + L-glutamate
-
 ir
6.3.4.2 additional information the enzyme can utilize NH3 as a substrate Trypanosoma brucei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.2 ? x * 70000, SDS-PAGE Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
6.3.4.2 CTP synthase
-
 Trypanosoma brucei
6.3.4.2 CTPS
-
 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.2 ATP
-
 Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.3.4.2 0.1
-
 in HEPES buffer (70 mM, pH 7.3), at 37°C Trypanosoma brucei uric acid
6.3.4.2 0.38
-
 in HEPES buffer (70 mM, pH 7.3), at 37°C Trypanosoma brucei guanosine
6.3.4.2 0.46
-
 in HEPES buffer (70 mM, pH 7.3), at 37°C Trypanosoma brucei GTP
6.3.4.2 0.48
-
 in HEPES buffer (70 mM, pH 7.3), at 37°C Trypanosoma brucei Caffeine