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Literature summary extracted from
- Enzymatic synthesis of L-norephedrine by coupling recombinant pyruvate decarboxylase and omega-transaminase (2014), Appl. Microbiol. Biotechnol., 98, 7399-7408.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.1.1 | recombinant expression in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.1 | Mg2+ | required | Saccharomyces cerevisiae |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.1 | 62000 | - |
x * 62000, recombinant enzyme, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.1 | Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.1 | benzaldehyde + pyruvate | - |
Saccharomyces cerevisiae | L-phenylacetylcarbinol + CO2 | - |
? | |
| 4.1.1.1 | additional information | enzymatic stereoselective synthesis of L-norephedrine by coupling recombinant R-selective pyruvate decarboxylase from from Saccharomyces cerevisiae and an S-selective omega-transaminase from Vibrio fluvialis JS17, method optimization, overview | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.1.1 | ? | x * 62000, recombinant enzyme, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.1.1 | R-selective pyruvate decarboxylase | - |
Saccharomyces cerevisiae |
| 4.1.1.1 | SCPDC | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.1 | 30 | - |
synthesis of L-norephedrine in a coupled reaction with omega-transaminase from Vibrio fluvialis JS17 | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.1 | 8 | - |
synthesis of L-norephedrine in a coupled reaction with omega-transaminase from Vibrio fluvialis JS17 | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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