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Literature summary extracted from

  • Choo, Y.M.; Pelletier, J.; Atungulu, E.; Leal, W.S.
    Identification and characterization of an antennae-specific aldehyde oxidase from the navel orangeworm (2013), PLoS ONE, 8, e67794.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.3.1 expression of recombinant AtraAOX2 with His6-tag at the C-terminal end in a baculovirus expression system Amyelois transitella

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.1 2,4-dinitrophenol 50 microM, 41.7% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 4-hydroxymercuribenzoate 50 microM, 55.3% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 antimycin A 10 microM, 45.6% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 beta-ecdysone 50 microM, 30.1% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 beta-estradiol 50 microM, 60.2% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 Digitonin 125 microM, 69.9% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 ethanol 2% (v/v), 62.1% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 Isopropanol 2% (v/v), 67.9% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 menadione 100 microM, 63.1% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 methanol 2% (v/v), 33.9% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 additional information hydroquinone and sodium deoxycholate have no inhibitory effect Amyelois transitella
1.2.3.1 potassium cyanide 1 mM, 71.8% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 Quinacrine 1 mM, 34.9% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 rotenone 10 microM, 89.3% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 Sodium azide 5 mM, 52.4% residual AtraAOX2 activity Amyelois transitella
1.2.3.1 Triton X-100 0.01% (v/v), 71.8% residual AtraAOX2 activity Amyelois transitella

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.3.1 140000
-
 SDS-PAGE, AtraAOX2 monomer still shows strong activity Amyelois transitella
1.2.3.1 280000
-
 Western blot analysis Amyelois transitella

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.1 Amyelois transitella S5FPI8 navel orangeworm
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.1 by Ni-NTA column chromatography Amyelois transitella

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.3.1 antenna male and female Amyelois transitella
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.1 (2E)-hex-2-enal + H2O + O2
-
 Amyelois transitella (2E)-hex-2-enoic acid + H2O2
-
 ?
1.2.3.1 (2E)-non-2-enal + H2O + O2
-
 Amyelois transitella (2E)-non-2-enoic acid + H2O2
-
 ?
1.2.3.1 acetaldehyde + H2O + O2
-
 Amyelois transitella acetic acid + H2O2
-
 ?
1.2.3.1 acrolein + H2O + O2 results indicate that AtraAOX2 also functions as a xenobiotic-degrading enzyme Amyelois transitella ?
-
 ?
1.2.3.1 benzaldehyde + H2O + O2
-
 Amyelois transitella benzoic acid + H2O2
-
 ?
1.2.3.1 citral + H2O + O2 low AtraAOX2 activity Amyelois transitella (2E)-3,7-dimethylocta-2,6-dienoic acid + H2O2
-
 ?
1.2.3.1 heptanal + H2O + O2
-
 Amyelois transitella heptanoic acid + H2O2
-
 ?
1.2.3.1 hexanal + H2O + O2
-
 Amyelois transitella hexanoic acid + H2O2
-
 ?
1.2.3.1 additional information AtraAOX2 shows strong activity on aldehyde substrates derived from plants, but weak activity on Z11Z13–16Ald, AtraAOX2 does not degrade the carbamate insecticide aldicarb Amyelois transitella ?
-
 ?
1.2.3.1 nonanal + H2O + O2
-
 Amyelois transitella nonanoic acid + H2O2
-
 ?
1.2.3.1 propanal + H2O + O2 highest AtraAOX2 activity Amyelois transitella propanoic acid + H2O2
-
 ?
1.2.3.1 vanillin + H2O + O2 low AtraAOX2 activity Amyelois transitella vanillic acid + H2O2
-
 ?
1.2.3.1 xanthine + H2O + O2 lowest AtraAOX2 activity Amyelois transitella ? + H2O2
-
 ?
1.2.3.1 Z11Z13-16Ald + H2O + O2 low AtraAOX2 activity Amyelois transitella ? + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.3.1 homodimer 2 * 140000, SDS-PAGE Amyelois transitella

Synonyms

EC Number Synonyms Comment Organism
1.2.3.1 antennae-specific aldehyde oxidase
-
 Amyelois transitella
1.2.3.1 AtraAOX2
-
 Amyelois transitella

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.3.1 20 60 relatively stable from 20 to 60°C but activity drops dramatically at 70°C, AtraAOX2 preheated at 60°C, shows biphasic inactivation, with a rapid decrease from 0 to 10 min, and monotonous decrease thereafter, with an estimated half-life of 33 min Amyelois transitella

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.1 8
-
 enzyme activity tested with three different buffers at pH 8, but no significant difference observed Amyelois transitella

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.3.1 4 8
-
 Amyelois transitella

General Information

EC Number General Information Comment Organism
1.2.3.1 physiological function AtraAOX2 could be involved in detoxifying plant-derived toxic aldehydes and aldehyde-containing pesticides Amyelois transitella