EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.3.1 | to 2.9 A resolution, used for elucidation of putative reaction mechanism of aldehyde oxidases | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.3.1 | A807V | does not affect the kinetic constants with smaller substrates like benzaldehyde or phthalazine, but affinity for bulkier substrates like phenanthridine decreases, whereas the catalytic efficiency is slightly raised | Mus musculus |
1.2.3.1 | E1266Q | complete loss of activity with different N-heterocyclic compounds as substrates, 60% reduction of enzyme activity with benzaldehyde | Mus musculus |
1.2.3.1 | K889H | 23fold decrease in the catalytic efficiency using benzaldehyde and phthalazine as substrates, but the Km-values remain the same | Mus musculus |
1.2.3.1 | Y885M | kinetic constants remain mainly the same with small hydrophobic substrates like benzaldehyde and phthalazine, bulkier substrates like phenanthridine or more charged substrates like N1-methylnicotinamide are converted with higher efficiency | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.1 | benzamidine | - |
Mus musculus | |
1.2.3.1 | menadione | - |
Mus musculus | |
1.2.3.1 | norharmane | - |
Mus musculus | |
1.2.3.1 | raloxifene | - |
Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.3.1 | additional information | - |
additional information | further kinetic parameters of phthalazine-DCPIP reaction in the absence or presence of the inhibitors benzamidine, menadione, norharmane, and raloxifene available in the publication | Mus musculus | |
1.2.3.1 | 0.0011 | - |
Phthalazine | K889H mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0014 | - |
Phthalazine | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0023 | - |
benzaldehyde | K889H mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0025 | - |
benzaldehyde | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0027 | - |
Phthalazine | A807V mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0029 | - |
benzaldehyde | A807V mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0029 | - |
Phthalazine | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0032 | - |
Phthalazine | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0046 | - |
benzaldehyde | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0063 | - |
benzaldehyde | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0118 | - |
phenanthridine | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0161 | - |
phenanthridine | K889H mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0255 | - |
N1-methylnicotinamide | K889H mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0323 | - |
phenanthridine | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0334 | - |
phenanthridine | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0359 | - |
N1-methylnicotinamide | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0827 | - |
N1-methylnicotinamide | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0863 | - |
benzaldehyde | E1266Q mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.1234 | - |
N1-methylnicotinamide | A807V mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.1285 | - |
N1-methylnicotinamide | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.1495 | - |
phenanthridine | A807V mutant, pH 8.0, 37°C | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.3.1 | Mus musculus | G3X982 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.3.1 | mAOX3 purified from CD1 mouse liver as well as from a heterologous expression system from Escherichia coli, recombinant mAOX3 expressed as an N-terminal fusion protein with a His6 tag | Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.3.1 | liver | - |
Mus musculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.3.1 | benzaldehyde + 2,6-dichlorophenol indophenol | natural electron acceptor of enzyme is molecular oxygen, DCPIP i.e., 2,6-dichlorophenol indophenol | Mus musculus | ? | - |
? | |
1.2.3.1 | N1-methylnicotinamide + 2,6-dichlorophenol indophenol | natural electron acceptor of enzyme is molecular oxygen, DCPIP i.e., 2,6-dichlorophenol indophenol | Mus musculus | ? | - |
? | |
1.2.3.1 | phenanthridine + H2O + O2 | - |
Mus musculus | ? | - |
? | |
1.2.3.1 | phthalazine + 2,6-dichlorophenol indophenol | natural electron acceptor of enzyme is molecular oxygen, DCPIP i.e., 2,6-dichlorophenol indophenol | Mus musculus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.3.1 | homodimer | each monomer comprises 1335 residues and can be divided into three major domains, a small 20000 Da N-terminal domain that harbors the two FeS clusters and connects to the 40000 Da FAD binding domain and finally the C-terminal 90000 Da domain containing the Moco binding site, with the molybdenum catalytic center | Mus musculus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.3.1 | AOX3 | - |
Mus musculus |
1.2.3.1 | mAOX3 | - |
Mus musculus |
1.2.3.1 | mouse liver aldehyde oxidase 3 | - |
Mus musculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.3.1 | additional information | - |
additional information | further kinetic parameters of phthalazine-DCPIP reaction in the absence or presence of the inhibitors benzamidine, menadione, norharmane, and raloxifene available in the publication | Mus musculus | |
1.2.3.1 | 0.052 | - |
N1-methylnicotinamide | K889H mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.17 | - |
benzaldehyde | E1266Q mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.187 | - |
N1-methylnicotinamide | A807V mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.197 | - |
Phthalazine | K889H mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.245 | - |
N1-methylnicotinamide | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.32 | - |
benzaldehyde | K889H mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.39 | - |
N1-methylnicotinamide | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.45 | - |
N1-methylnicotinamide | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.683 | - |
benzaldehyde | A807V mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.685 | - |
Phthalazine | A807V mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.685 | - |
Phthalazine | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.69 | - |
Phthalazine | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.698 | - |
benzaldehyde | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.727 | - |
benzaldehyde | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.815 | - |
Phthalazine | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.862 | - |
phenanthridine | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.957 | - |
benzaldehyde | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 1.33 | - |
phenanthridine | K889H mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 3.63 | - |
phenanthridine | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 4.45 | - |
phenanthridine | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 5.25 | - |
phenanthridine | A807V mutant, pH 8.0, 37°C | Mus musculus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.1 | FAD | - |
Mus musculus | |
1.2.3.1 | iron-sulfur centre | two centers, FeSI and FeSII | Mus musculus | |
1.2.3.1 | molybdenum cofactor | - |
Mus musculus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.3.1 | 0.00125 | - |
menadione | uncompetitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0122 | - |
raloxifene | competitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.018 | - |
norharmane | competitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0225 | - |
benzamidine | competitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 0.0591 | - |
raloxifene | uncompetitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37°C | Mus musculus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.3.1 | 1.52 | - |
N1-methylnicotinamide | A807V mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 1.92 | - |
N1-methylnicotinamide | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 1.97 | - |
benzaldehyde | E1266Q mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 2 | - |
N1-methylnicotinamide | K889H mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 4.72 | - |
N1-methylnicotinamide | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 12.5 | - |
N1-methylnicotinamide | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 26.7 | - |
phenanthridine | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 35.2 | - |
phenanthridine | A807V mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 82.8 | - |
phenanthridine | K889H mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 133.2 | - |
phenanthridine | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 136.7 | - |
benzaldehyde | K889H mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 151.8 | - |
benzaldehyde | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 158 | - |
benzaldehyde | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 175.5 | - |
Phthalazine | K889H mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 235.7 | - |
benzaldehyde | A807V mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 238 | - |
Phthalazine | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 253.7 | - |
Phthalazine | A807V mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 254.7 | - |
Phthalazine | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 279.3 | - |
benzaldehyde | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 307.8 | - |
phenanthridine | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
1.2.3.1 | 489.3 | - |
Phthalazine | mAOX3 wild type, pH 8.0, 37°C | Mus musculus |