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Literature summary extracted from

  • Sonawane, P.; Vishwakarma, R.K.; Khan, B.M.
    Biochemical characterization of recombinant cinnamoyl CoA reductase 1 (Ll-CCRH1) from Leucaena leucocephala (2013), Int. J. Biol. Macromol., 58, 154-159.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.44 Triton X-100 106% activity at 1% (v/v) Leucaena leucocephala
1.2.1.44 Tween 20 113% activity at 1% (v/v) Leucaena leucocephala

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.44 expressed in Escherichia coli BL21(DE3) cells Leucaena leucocephala

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.44 Ca2+ 86% residual activity at 2 mM Leucaena leucocephala
1.2.1.44 Co2+ 42% residual activity at 2 mM Leucaena leucocephala
1.2.1.44 Cs2+ 83% residual activity at 2 mM Leucaena leucocephala
1.2.1.44 Cu2+ 49% residual activity at 2 mM Leucaena leucocephala
1.2.1.44 Fe2+ 92% residual activity at 2 mM Leucaena leucocephala
1.2.1.44 Hg2+ complete inhibition at 2 mM Leucaena leucocephala
1.2.1.44 Mg2+ 74% residual activity at 2 mM Leucaena leucocephala
1.2.1.44 Mn2+ 21% residual activity at 2 mM Leucaena leucocephala
1.2.1.44 Ni2+ 25% residual activity at 2 mM Leucaena leucocephala
1.2.1.44 SDS 6% residual activity at 1% (v/v) Leucaena leucocephala
1.2.1.44 Zn2+ 45% residual activity at 2 mM Leucaena leucocephala

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.44 0.031
-
 coniferaldehyde in 100 mM phosphate buffer pH 7.8, at 30°C Leucaena leucocephala
1.2.1.44 0.036
-
 feruloyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 0.039
-
 sinapaldehyde in 100 mM phosphate buffer pH 7.8, at 30°C Leucaena leucocephala
1.2.1.44 0.048
-
 caffeoyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 0.05
-
 coniferaldehyde in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 0.057
-
 sinapoyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 0.06
-
 coumaroyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 0.067
-
 sinapaldehyde in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.44 38000
-
 x * 38000, SDS-PAGE Leucaena leucocephala

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.44 caffeoyl-CoA + NADPH + H+ Leucaena leucocephala
-
 caffealdehyde + CoA + NADP+
-
 r
1.2.1.44 coniferaldehyde + CoA + NADP+ Leucaena leucocephala
-
 feruloyl-CoA + NADPH + H+
-
 r
1.2.1.44 coumaroyl-CoA + NADPH + H+ Leucaena leucocephala
-
 coumaric aldehyde + CoA + NADP+
-
 r
1.2.1.44 feruloyl-CoA + NADPH + H+ Leucaena leucocephala best substrate coniferaldehyde + CoA + NADP+
-
 r
1.2.1.44 sinapaldehyde + CoA + NADP+ Leucaena leucocephala
-
 sinapoyl-CoA + NADPH + H+
-
 r
1.2.1.44 sinapoyl-CoA + NADPH + H+ Leucaena leucocephala
-
 sinapaldehyde + CoA + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.44 Leucaena leucocephala
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.44
-
 Leucaena leucocephala

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.44 caffeoyl-CoA + NADPH + H+
-
 Leucaena leucocephala caffealdehyde + CoA + NADP+
-
 r
1.2.1.44 coniferaldehyde + CoA + NADP+
-
 Leucaena leucocephala feruloyl-CoA + NADPH + H+
-
 r
1.2.1.44 coumaroyl-CoA + NADPH + H+
-
 Leucaena leucocephala coumaric aldehyde + CoA + NADP+
-
 r
1.2.1.44 feruloyl-CoA + NADPH + H+ best substrate Leucaena leucocephala coniferaldehyde + CoA + NADP+
-
 r
1.2.1.44 sinapaldehyde + CoA + NADP+
-
 Leucaena leucocephala sinapoyl-CoA + NADPH + H+
-
 r
1.2.1.44 sinapoyl-CoA + NADPH + H+
-
 Leucaena leucocephala sinapaldehyde + CoA + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.44 ? x * 38000, SDS-PAGE Leucaena leucocephala

Synonyms

EC Number Synonyms Comment Organism
1.2.1.44 CCRH1
-
 Leucaena leucocephala
1.2.1.44 cinnamoyl CoA reductase 1
-
 Leucaena leucocephala

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.44 25
-
 below and above 25°C, gradual loss in the enzyme activity is recorded Leucaena leucocephala

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.44 20 40 the enzyme is stable for 60 min at 25°C and at optimum temperature 30°C, stability is observed for 40 min. However, at 20°C and 35°C, 36% and 42% loss in activity is observed after 60 min. Above 40°C, significant loss in Ll-CCRH1 activity is observed within 30 min Leucaena leucocephala

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.44 81
-
 sinapaldehyde in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 94
-
 coniferaldehyde in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 105
-
 coumaroyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 113
-
 caffeoyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 131
-
 sinapoyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 164
-
 feruloyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 210
-
 sinapaldehyde in 100 mM phosphate buffer pH 7.8, at 30°C Leucaena leucocephala
1.2.1.44 236
-
 coniferaldehyde in 100 mM phosphate buffer pH 7.8, at 30°C Leucaena leucocephala

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.44 7.5
-
 forward reaction Leucaena leucocephala
1.2.1.44 7.8
-
 reverse reaction Leucaena leucocephala

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.44 6 7 the enzyme is most stable around pH 6.5 at 25°C for 90 min, while at pH 6.0 and 7.0, the enzyme is stable for 45 and 60 min, respectively Leucaena leucocephala

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.44 NADP+
-
 Leucaena leucocephala
1.2.1.44 NADPH
-
 Leucaena leucocephala

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.44 1200
-
 sinapaldehyde in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 1700
-
 coumaroyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 1900
-
 coniferaldehyde in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 2300
-
 sinapoyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 2400
-
 caffeoyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 4600
-
 feruloyl-CoA in 100 mM phosphate buffer pH 6.5, at 30°C Leucaena leucocephala
1.2.1.44 5300
-
 sinapaldehyde in 100 mM phosphate buffer pH 7.8, at 30°C Leucaena leucocephala
1.2.1.44 7500
-
 coniferaldehyde in 100 mM phosphate buffer pH 7.8, at 30°C Leucaena leucocephala