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Literature summary extracted from

  • Mitsui, R.; Hirota, M.; Tsuno, T.; Tanaka, M.
    Purification and characterization of vanillin dehydrogenases from alkaliphile Micrococcus sp. TA1 and neutrophile Burkholderia cepacia TM1 (2010), FEMS Microbiol. Lett., 303, 41-47.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.67 Ag+ 100% inhibition with 1 mM Burkholderia cepacia
1.2.1.67 Ag+ 100% inhibition with 1 mM Micrococcus sp.
1.2.1.67 EDTA 40% inhibition by 1 mM and 45% inhibition at 5 mM Micrococcus sp.
1.2.1.67 Mg2+ 1.9fold increased enzyme activity with 1 mM Micrococcus sp.
1.2.1.67 additional information no effect of EDTA on enzyme activity; no inhibition with 1 mM Cu2+, Fe3+, Fe2+, Zn2+, Mn2+, and Co2+ Burkholderia cepacia
1.2.1.67 additional information no inhibition with 1 mM Cu2+, Fe3+, Fe2+, Zn2+, Mn2+, and Co2+ Micrococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.67 0.004
-
 Vanillin pH 7, temperature not specified in the publication Burkholderia cepacia
1.2.1.67 0.007
-
 Vanillin pH 7, temperature not specified in the publication Micrococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.67 57000
-
 2 * 57000, SDS-PAGE Burkholderia cepacia
1.2.1.67 57000
-
 4 * 57000, SDS-PAGE Micrococcus sp.
1.2.1.67 110000
-
 gel filtration Burkholderia cepacia
1.2.1.67 250000
-
 gel filtration Micrococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.67 Burkholderia cepacia
-
-
-
1.2.1.67 Burkholderia cepacia TM1
-
-
-
1.2.1.67 Micrococcus sp.
-
-
-
1.2.1.67 Micrococcus sp. TA1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.67 by DEAE-Sepharose FF column, butyl-Sepharose column and Resource Q column chromatography Burkholderia cepacia
1.2.1.67 by DEAE-Sepharose FF column, butyl-Sepharose column and Resource Q column chromatography Micrococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.67 4-hydroxybenzaldehyde + NADP+ + H2O 70% relative activity compared to vanillin Burkholderia cepacia 4-hydroxybenzoate + NADPH + 2 H+
-
 ?
1.2.1.67 4-hydroxybenzaldehyde + NADP+ + H2O 77% relative activity compared to vanillin Micrococcus sp. 4-hydroxybenzoate + NADPH + 2 H+
-
 ?
1.2.1.67 4-hydroxybenzaldehyde + NADP+ + H2O 77% relative activity compared to vanillin Micrococcus sp. TA1 4-hydroxybenzoate + NADPH + 2 H+
-
 ?
1.2.1.67 4-hydroxybenzaldehyde + NADP+ + H2O 70% relative activity compared to vanillin Burkholderia cepacia TM1 4-hydroxybenzoate + NADPH + 2 H+
-
 ?
1.2.1.67 isovanillin + NADP+ + H2O 285% relative activity compared to vanillin Micrococcus sp. isovanillate + NADPH + 2 H+
-
 ?
1.2.1.67 isovanillin + NADP+ + H2O 48% relative activity compared to vanillin Burkholderia cepacia isovanillate + NADPH + 2 H+
-
 ?
1.2.1.67 isovanillin + NADP+ + H2O 285% relative activity compared to vanillin Micrococcus sp. TA1 isovanillate + NADPH + 2 H+
-
 ?
1.2.1.67 isovanillin + NADP+ + H2O 48% relative activity compared to vanillin Burkholderia cepacia TM1 isovanillate + NADPH + 2 H+
-
 ?
1.2.1.67 protocatechualdehyde + NADP+ + H2O 146% relative activity compared to vanillin Micrococcus sp. protocatechuate + NADPH + 2 H+
-
 ?
1.2.1.67 protocatechualdehyde + NADP+ + H2O 94% relative activity compared to vanillin Burkholderia cepacia protocatechuate + NADPH + 2 H+
-
 ?
1.2.1.67 protocatechualdehyde + NADP+ + H2O 146% relative activity compared to vanillin Micrococcus sp. TA1 protocatechuate + NADPH + 2 H+
-
 ?
1.2.1.67 protocatechualdehyde + NADP+ + H2O 94% relative activity compared to vanillin Burkholderia cepacia TM1 protocatechuate + NADPH + 2 H+
-
 ?
1.2.1.67 syringaldehyde + NADP+ + H2O 23% relative activity compared to vanillin Micrococcus sp. syringate + NADPH + 2 H+
-
 ?
1.2.1.67 syringaldehyde + NADP+ + H2O 29% relative activity compared to vanillin Burkholderia cepacia syringate + NADPH + 2 H+
-
 ?
1.2.1.67 syringaldehyde + NADP+ + H2O 23% relative activity compared to vanillin Micrococcus sp. TA1 syringate + NADPH + 2 H+
-
 ?
1.2.1.67 syringaldehyde + NADP+ + H2O 29% relative activity compared to vanillin Burkholderia cepacia TM1 syringate + NADPH + 2 H+
-
 ?
1.2.1.67 vanillin + NAD+ + H2O higher activity with NAD+ than with NADP+, relative activity with NADP1 is approximately 10% Burkholderia cepacia vanillate + NADH + 2 H+
-
 ?
1.2.1.67 vanillin + NAD+ + H2O higher activity with NAD+ than with NADP+, relative activity with NADP1 is approximately 10% Burkholderia cepacia TM1 vanillate + NADH + 2 H+
-
 ?
1.2.1.67 vanillin + NADP+ + H2O no activity with NAD+, strain TA1 exhibits oxidation activity specifically under alkaline conditions Micrococcus sp. vanillate + NADPH + 2 H+
-
 ?
1.2.1.67 vanillin + NADP+ + H2O no activity with NAD+, strain TA1 exhibits oxidation activity specifically under alkaline conditions Micrococcus sp. TA1 vanillate + NADPH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.67 homodimer 2 * 57000, SDS-PAGE Burkholderia cepacia
1.2.1.67 homotetramer 4 * 57000, SDS-PAGE Micrococcus sp.

Synonyms

EC Number Synonyms Comment Organism
1.2.1.67 VDH
-
 Burkholderia cepacia
1.2.1.67 VDH
-
 Micrococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.67 30
-
-
 Micrococcus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.67 35 60 high activity across a wide range of temperature Burkholderia cepacia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.67 30
-
 stable up to 30 min Micrococcus sp.
1.2.1.67 35
-
 stable up to 30 min Burkholderia cepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.67 9 10
-
 Burkholderia cepacia
1.2.1.67 9 10
-
 Micrococcus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.67 6 9 30-min incubation at 30°C Burkholderia cepacia
1.2.1.67 7 8 30 min incubation at 30°C Micrococcus sp.