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Literature summary extracted from

  • Si, J.; Peng, F.; Cui, B.
    Purification, biochemical characterization and dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens (2013), Biores. Technol., 128, 49-57.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Al3+ 11.6% inhibition at 1 mM Trametes pubescens
1.10.3.2 Ba2+ 10% inhibition at 1 mM Trametes pubescens
1.10.3.2 dithiothreitol
-
 Trametes pubescens
1.10.3.2 EDTA
-
 Trametes pubescens
1.10.3.2 L-cysteine
-
 Trametes pubescens
1.10.3.2 Sodium azide
-
 Trametes pubescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Trametes pubescens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ activates slightly at 1 mM Trametes pubescens
1.10.3.2 additional information the laccase is highly metal-tolerant. No or poor effects by Mn2+, Na+, Zn2+, Mg2+, Ca2+, K+, Fe2+, and Fe3+ Trametes pubescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 68000
-
 1 * 68000, SDS-PAGE Trametes pubescens

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trametes pubescens
-
 collected from Chebaling Nature Reserve of Guangdong Province in China
-
1.10.3.2 Trametes pubescens Cui 7571
-
 collected from Chebaling Nature Reserve of Guangdong Province in China
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native extracellular enzyme 16fold from cell-free supernatant by ammonium sulfate fractionation, PEG 20000 concentration, anion exchange chromatography, and gel filtration Trametes pubescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 18.54
-
 purified native extracellular enzyme, pH 5.0, 50°C Trametes pubescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Trametes pubescens ?
-
 ?
1.10.3.2 2,6-dimethoxy phenol + O2
-
 Trametes pubescens ?
-
 ?
1.10.3.2 4 catechol + O2
-
 Trametes pubescens 4 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.2 4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 i.e. syringaldazine Trametes pubescens ?
-
 ?
1.10.3.2 ferulic acid + O2
-
 Trametes pubescens ?
-
 ?
1.10.3.2 ferulic acid + O2
-
 Trametes pubescens Cui 7571 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Trametes pubescens ?
-
 ?
1.10.3.2 hydroquinone + O2
-
 Trametes pubescens ?
-
 ?
1.10.3.2 hydroquinone + O2
-
 Trametes pubescens Cui 7571 ?
-
 ?
1.10.3.2 L-DOPA + O2
-
 Trametes pubescens ?
-
 ?
1.10.3.2 L-DOPA + O2
-
 Trametes pubescens Cui 7571 ?
-
 ?
1.10.3.2 additional information substrate specificity, overview. No activity with tyrosine. Dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens, overview Trametes pubescens ?
-
 ?
1.10.3.2 additional information substrate specificity, overview. No activity with tyrosine. Dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens, overview Trametes pubescens Cui 7571 ?
-
 ?
1.10.3.2 phenol + O2
-
 Trametes pubescens ?
-
 ?
1.10.3.2 pyrogallol + O2
-
 Trametes pubescens ?
-
 ?
1.10.3.2 pyrogallol + O2
-
 Trametes pubescens Cui 7571 ?
-
 ?
1.10.3.2 veratryl alcohol + O2
-
 Trametes pubescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 68000, SDS-PAGE Trametes pubescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
-
 Trametes pubescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 5
-
-
 Trametes pubescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 4.5 11 activity range Trametes pubescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 4.5 10 the purified enzyme is highly stable over a broad range of pH values ranging from pH 4.5 to pH 10.0, maintaining 75% of its original activity after incubation at 25°C for 72 h, it is highly stable and resistant under alkaline conditions, with pH values ranging from pH 7.0 to pH 10.0 Trametes pubescens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 8340
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 50°C Trametes pubescens