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Literature summary extracted from

  • Zhang, Y.; Livingstone, J.; Hirasawa, E.
    Purification and characterisation of monoamine oxidase from Avena sativa (2012), Acta Physiol. Plant., 34, 1411-1419.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.4 benzaldehyde strong competitive inhibitor Avena sativa
1.4.3.4 additional information enzyme activity is not inhibited by pargyline, clorgyline, semicarbazide, or sodium diethyldithiocarbamate Avena sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.4 0.27
-
 benzylamine at pH 7.0 and 37°C Avena sativa
1.4.3.4 0.71
-
 2-Phenylethylamine at pH 7.0 and 37°C Avena sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.4 32000
-
 1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE Avena sativa
1.4.3.4 55000
-
 1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE Avena sativa
1.4.3.4 58000
-
 1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE Avena sativa
1.4.3.4 78000
-
 1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE Avena sativa
1.4.3.4 220000
-
 gel filtration Avena sativa

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.4 Avena sativa
-
 cultivar Miechikara
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.4 Sephadex G-25 gel filtration, DEAE Sephacel gel filtration, hydroxyapatitecolumn chromatography, Mono Q column chromatography, and TSK-GEL filtration Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.4 seedling
-
 Avena sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.4 0.00015
-
 crude extract, at pH 7.0 and 37°C Avena sativa
1.4.3.4 0.02
-
 125fold purified enzyme, at pH 7.0 and 37°C Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.4 2-phenylethylamine + H2O + O2 the enzyme shows high substrate specificity for phenethylamine (100% activity) Avena sativa 2-phenylacetaldehyde + NH3 + H2O2
-
 ?
1.4.3.4 benzylamine + H2O + O2 60% activity compared to 2-phenylethylamine Avena sativa benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.4 additional information no activity with tyramine, tryptamine, serotonin, dopamine, ethanolamine, histamine, ethylamine, n-propylamine, n-butylamine, n-pentylamine, putrescine, and spermidine Avena sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.4 heterotetramer 1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE Avena sativa

Synonyms

EC Number Synonyms Comment Organism
1.4.3.4 MAO
-
 Avena sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.4 50
-
-
 Avena sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.4 37 80 the purified enzyme retains its activity at temperatures up to 50°C, but loses activity quickly after 10 min incubation at temperaturesabove 50°C Avena sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.4 7
-
-
 Avena sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.4 FAD the enzyme contains 2 mol of FAD per 220000 g of enzyme Avena sativa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.4 0.003
-
 benzaldehyde at pH 7.0 and 37°C Avena sativa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.4 Avena sativa isoelectric focusing
-
 6.3

Expression

EC Number Organism Comment Expression
1.4.3.4 Avena sativa enzyme activity increases up to day 3 after germination, thereafter decreasing again up