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Literature summary extracted from

  • Hupert-Kocurek, K.; Guzik, U.; Wojcieszynska, D.
    Characterization of catechol 2,3-dioxygenase from Planococcus sp. strain S5 induced by high phenol concentration (2012), Acta Biochim. Pol., 59, 345-351.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 DNA and amino acid sequence determination and analysis Planococcus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.0427
-
phenol pH 7.5, 40°C Planococcus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Planococcus sp. (in: Bacteria)
-
2-hydroxymuconate-6-semialdehyde
-
?
1.13.11.2 catechol + O2 Planococcus sp. (in: Bacteria) S5
-
2-hydroxymuconate-6-semialdehyde
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Planococcus sp. (in: Bacteria) E7DDG2
-
-
1.13.11.2 Planococcus sp. (in: Bacteria) S5 E7DDG2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.2 culture condition:phenol-grown cell
-
Planococcus sp. (in: Bacteria)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-methylcatechol + O2
-
Planococcus sp. (in: Bacteria) ?
-
?
1.13.11.2 3-methylcatechol + O2
-
Planococcus sp. (in: Bacteria) S5 ?
-
?
1.13.11.2 4-chlorocatechol + O2
-
Planococcus sp. (in: Bacteria) ?
-
?
1.13.11.2 4-chlorocatechol + O2
-
Planococcus sp. (in: Bacteria) S5 ?
-
?
1.13.11.2 4-methylcatechol + O2
-
Planococcus sp. (in: Bacteria) ?
-
?
1.13.11.2 4-methylcatechol + O2
-
Planococcus sp. (in: Bacteria) S5 ?
-
?
1.13.11.2 catechol + O2
-
Planococcus sp. (in: Bacteria) 2-hydroxymuconate-6-semialdehyde
-
?
1.13.11.2 catechol + O2
-
Planococcus sp. (in: Bacteria) S5 2-hydroxymuconate-6-semialdehyde
-
?
1.13.11.2 phenol + O2
-
Planococcus sp. (in: Bacteria) ?
-
?
1.13.11.2 phenol + O2
-
Planococcus sp. (in: Bacteria) S5 ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.2 60
-
-
Planococcus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.2 50
-
loss of 30% activity after 20 min Planococcus sp. (in: Bacteria)
1.13.11.2 60
-
half-life is 10 min Planococcus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 8
-
-
Planococcus sp. (in: Bacteria)

General Information

EC Number General Information Comment Organism
1.13.11.2 additional information Planococcus sp. strain S5 grown on 1 or 2 mM phenol shows activity of both a catechol 1,2- and catechol 2,3-dioxygenase while at a higher concentrations of phenol only catechol 2,3-dioxygenase activity is observed Planococcus sp. (in: Bacteria)