EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.21 | gene BfSS1, overexpression in Bupleurum falcatum using transfection via Agrobacterium tumefaciens strain GV3101 | Bupleurum falcatum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.21 | additional information | expression in sense and antisense orientation, phenotypes, overview | Bupleurum falcatum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.21 | 47000 | - |
x * 47000, about, sequence calculation | Bupleurum falcatum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.17 | squalene + AH2 + O2 | Bupleurum falcatum | - |
(S)-squalene-2,3-epoxide + A + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.17 | Bupleurum falcatum | - |
- |
- |
2.5.1.21 | Bupleurum falcatum | Q32R64 | gene BfSS1 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.17 | root | - |
Bupleurum falcatum | - |
2.5.1.21 | root | - |
Bupleurum falcatum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.17 | squalene + AH2 + O2 | - |
Bupleurum falcatum | (S)-squalene-2,3-epoxide + A + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.21 | ? | x * 47000, about, sequence calculation | Bupleurum falcatum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.17 | squalene epoxidase | - |
Bupleurum falcatum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.17 | NADPH | - |
Bupleurum falcatum |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.14.17 | Bupleurum falcatum | overexpression of the squalene synthase 1 gene in the sense orientation increases the mRNA accumulation of downstream genes such as squalene epoxidase. Methyl jasmonate treatment of wild type roots enhances the expression of the enzyme | up |
2.5.1.21 | Bupleurum falcatum | BfSS1 mRNA accumulates ubiquitously in plant organs and markedly increases in roots after treatment with methyl jasmonate, abscisic acid, and ethephon | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.21 | metabolism | squalene synthase catalyzes the first enzymatic step of the central isoprenoid pathway in sterol and triterpenoid biosynthesis | Bupleurum falcatum |
2.5.1.21 | additional information | methyl jasmonate, abscisic acid, and ethephon induce the accumulation of BfSS1 mRNA, overexpression of the BfSS1 gene in the sense orientation in Bupleurum falcatum increases the mRNA accumulation of downstream genes such as squalene epoxidase and cycloartenol synthase but decreases the mRNA levels of beta-amyrin synthase, a triterpene synthase mRNA. Methyljasmonate treatment of transgenic roots overexpressing BfSS1 in the sense orientation fails to stimulate beta-amyrin synthase mRNA accumulation but still enhances saikosaponin and phytosterol production | Bupleurum falcatum |
2.5.1.21 | physiological function | squalene synthase functions as a key regulator in channeling the carbon flux into both the primary and secondary metabolite branches, and squalene synthase may play a regulatory role in directing triterpene intermediates and sterol pathways | Bupleurum falcatum |