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Literature summary extracted from

  • Chan, C.H.; Escalante-Semerena, J.C.
    ArsAB, a novel enzyme from Sporomusa ovata activates phenolic bases for adenosylcobamide biosynthesis (2011), Mol. Microbiol., 81, 952-967.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.21 expressed in Escherichia coli Sporomusa ovata
2.4.2.55
-
 Sporomusa ovata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.21 36180
-
 functional as a heterodimer, 1 * 36650 (ArsA) + 1 * 36180 (ArsB), SDS-PAGE Sporomusa ovata
2.4.2.21 36650
-
 functional as a heterodimer, 1 * 36650 (ArsA) + 1 * 36180 (ArsB), SDS-PAGE Sporomusa ovata
2.4.2.55 36650
-
 1 * 36650 (ArsA) + 1 * 361800 (ArsB), calculated from sequence Sporomusa ovata
2.4.2.55 66000
-
 gel filtration Sporomusa ovata
2.4.2.55 361800
-
 1 * 36650 (ArsA) + 1 * 361800 (ArsB), calculated from sequence Sporomusa ovata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.55 beta-nicotinate D-ribonucleotide + 4-cresol Sporomusa ovata the enzyme is involved in the biosynthesis of phenolic cobamides in Sporomusa ovata nicotinate + 4-methylphenyl 5-phospho-alpha-D-ribofuranoside
-
 ?
2.4.2.55 beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole Sporomusa ovata the enzyme is involved in the biosynthesis of phenolic cobamides in Sporomusa ovata nicotinate + alpha-ribazole 5'-phosphate
-
 ?
2.4.2.55 beta-nicotinate D-ribonucleotide + phenol Sporomusa ovata the enzyme is involved in the biosynthesis of phenolic cobamides in Sporomusa ovata nicotinate + phenyl 5-phospho-alpha-D-ribofuranoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.21 Sporomusa ovata F6MZ55 and F6MZ56 subunit ArsA: F6MZ55, subunit ArsB: F6MZ56
-
2.4.2.55 Sporomusa ovata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.21
-
 Sporomusa ovata
2.4.2.55
-
 Sporomusa ovata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.55 0.019
-
 pH 9.0, 37°C Sporomusa ovata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.21 5,6-dimethylbenzimidazole + beta-nicotinate D-ribonucleotide
-
 Sporomusa ovata nicotinate + N1-(5-phospho-alpha-D-ribosyl)-5,6-dimethylbenzimidazole
-
 ?
2.4.2.21 p-cresol + beta-nicotinate D-ribonucleotide
-
 Sporomusa ovata nicotinate + 4-hydroxyphenyl alpha-D-ribose 5-phosphate
-
 ?
2.4.2.21 phenol + beta-nicotinate D-ribonucleotide
-
 Sporomusa ovata nicotinate + phenyl alpha-D-ribose 5-phosphate
-
 ?
2.4.2.55 beta-nicotinate D-ribonucleotide + 4-cresol
-
 Sporomusa ovata nicotinate + 4-methylphenyl 5-phospho-alpha-D-ribofuranoside
-
 ?
2.4.2.55 beta-nicotinate D-ribonucleotide + 4-cresol the enzyme is involved in the biosynthesis of phenolic cobamides in Sporomusa ovata Sporomusa ovata nicotinate + 4-methylphenyl 5-phospho-alpha-D-ribofuranoside
-
 ?
2.4.2.55 beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole
-
 Sporomusa ovata nicotinate + alpha-ribazole 5'-phosphate
-
 ?
2.4.2.55 beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole the enzyme is involved in the biosynthesis of phenolic cobamides in Sporomusa ovata Sporomusa ovata nicotinate + alpha-ribazole 5'-phosphate
-
 ?
2.4.2.55 beta-nicotinate D-ribonucleotide + phenol
-
 Sporomusa ovata nicotinate + phenyl 5-phospho-alpha-D-ribofuranoside
-
 ?
2.4.2.55 beta-nicotinate D-ribonucleotide + phenol the enzyme is involved in the biosynthesis of phenolic cobamides in Sporomusa ovata Sporomusa ovata nicotinate + phenyl 5-phospho-alpha-D-ribofuranoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.21 heterodimer functional as a heterodimer, 1 * 36650 (ArsA) + 1 * 36180 (ArsB), SDS-PAGE Sporomusa ovata
2.4.2.55 heterodimer 1 * 36650 (ArsA) + 1 * 361800 (ArsB), calculated from sequence Sporomusa ovata

Synonyms

EC Number Synonyms Comment Organism
2.4.2.21 SoArsAB
-
 Sporomusa ovata
2.4.2.55 ArsAB
-
 Sporomusa ovata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.21 37
-
 assay at Sporomusa ovata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.21 9
-
-
 Sporomusa ovata
2.4.2.55 9
-
-
 Sporomusa ovata

General Information

EC Number General Information Comment Organism
2.4.2.55 physiological function the enzyme is involved in the biosynthesis of phenolic cobamides in Sporomusa ovata Sporomusa ovata