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Literature summary extracted from

  • Xie, X.; Xia, J.; He, K.; Lu, L.; Xu, Q.; Chen, N.
    Low-molecular-mass purine nucleoside phosphorylase: characterization and application in enzymatic synthesis of nucleoside antiviral drugs (2011), Biotechnol. Lett., 33, 1107-1112.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 expression in Escherichia coli Bacillus subtilis
2.4.2.1 expression in Escherichia coli Escherichia coli
2.4.2.1 expression in Escherichia coli Pseudoalteromonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.00031
-
 Inosine isoform of 816 amino acids, pH 7.0, 37°C Bacillus subtilis
2.4.2.1 0.00045
-
 Inosine pH 7.0, 37°C Pseudoalteromonas sp.
2.4.2.1 0.00085
-
 Inosine pH 7.0, 37°C Escherichia coli
2.4.2.1 0.012
-
 Inosine isoform of 702 amino acids, pH 7.0, 37°C Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Bacillus subtilis
-
-
-
2.4.2.1 Bacillus subtilis 168
-
-
-
2.4.2.1 Escherichia coli
-
-
-
2.4.2.1 Pseudoalteromonas sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 2'-deoxyadenosine + phosphate 12% of the activity with inosine Escherichia coli adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 2'-deoxyadenosine + phosphate isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 15% of the activity with inosine Bacillus subtilis adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 2'-deoxyadenosine + phosphate isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 15% of the activity with inosine Bacillus subtilis 168 adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 2'-deoxyadenosine + phosphate 10% of the activity with inosine Pseudoalteromonas sp. 2-deoxy-alpha-D-ribose 1-phosphate + adenine
-
 ?
2.4.2.1 2'-deoxyguanosine + phosphate 20% of the activity with inosine Escherichia coli guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 2'-deoxyguanosine + phosphate isoform of 816 amino acids, 17% of the activity with inosine, isoform of 702 amino acids, 18% of the activity with inosine Bacillus subtilis guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 2'-deoxyguanosine + phosphate isoform of 816 amino acids, 17% of the activity with inosine, isoform of 702 amino acids, 18% of the activity with inosine Bacillus subtilis 168 guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 2'-deoxyinosine + phosphate 22% of the activity with inosine Escherichia coli hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 2'-deoxyinosine + phosphate isoform of 816 amino acids, 23% of the activity with inosine, isoform of 702 amino acids, 31% of the activity with inosine Bacillus subtilis hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate 41% of the activity with inosine Escherichia coli adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate 45% of the activity with inosine Pseudoalteromonas sp. adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 48% of the activity with inosine Bacillus subtilis adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 48% of the activity with inosine Bacillus subtilis 168 adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 deoxyinosine + phosphate 14% of the activity with inosine Pseudoalteromonas sp. hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 deoxyinosine + phosphate 19% of the activity with inosine Pseudoalteromonas sp. hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate 56% of the activity with inosine Pseudoalteromonas sp. guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate 66% of the activity with inosine Escherichia coli guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate isoform of 816 amino acids, 87% of the activity with inosine, isoform of 702 amino acids, 46% of the activity with inosine Bacillus subtilis guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate isoform of 816 amino acids, 87% of the activity with inosine, isoform of 702 amino acids, 46% of the activity with inosine Bacillus subtilis 168 guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Bacillus subtilis hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Escherichia coli hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Pseudoalteromonas sp. hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Bacillus subtilis 168 hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 3900
-
 Inosine isoform of 702 amino acids, pH 7.0, 37°C Bacillus subtilis
2.4.2.1 6200
-
 Inosine pH 7.0, 37°C Escherichia coli
2.4.2.1 7000
-
 Inosine pH 7.0, 37°C Pseudoalteromonas sp.
2.4.2.1 12000
-
 Inosine isoform of 816 amino acids, pH 7.0, 37°C Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.1 74000
-
 Inosine pH 7.0, 37°C Escherichia coli
2.4.2.1 160000
-
 Inosine pH 7.0, 37°C Pseudoalteromonas sp.
2.4.2.1 320000
-
 Inosine isoform of 702 amino acids, pH 7.0, 37°C Bacillus subtilis
2.4.2.1 3900000
-
 Inosine isoform of 816 amino acids, pH 7.0, 37°C Bacillus subtilis