EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.21 | expressed in Escherichia coli | Sporomusa ovata |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.2.21 | phloroglucinol is identified from an additive screen as a compound that dramatically improves the size, stability, and diffraction properties of the crystals. Subsequent structural studies show that phloroglucinol binds in the active site of ArsA | Sporomusa ovata |
2.4.2.21 | the three-dimensional crystal structure of Sporomusa ovata ArsAB in its substrate-free form (2.1 A), and in complex with DMB (1.5 A), p-cresol (2.1 A), phloroglucinol (1.6 A), and phenol (2.4 A) is reported. Only the active site in ArsA is occupied by substrate. Analysis of the ArsB active site identified an arginyl side chain that may block access to the site | Sporomusa ovata |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.21 | I321S | specific activity with p-cresol and 5,6-dimethylbenzimidazole decreased | Sporomusa ovata |
2.4.2.21 | M87Q | effect of the M87Q mutation is most pronounced with respect to phenolic specificity, as the specific activity with p-cresol drops by 2.6fold while a 1.2fold increase in activity with 5,6-dimethylbenzimidazole is observed | Sporomusa ovata |
2.4.2.21 | M87Q/I321S | specific activity with p-cresol and 5,6-dimethylbenzimidazole highly decreased | Sporomusa ovata |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.21 | 0.05 | - |
phenol | pH 9.0, 37°C, wild-type | Sporomusa ovata | |
2.4.2.21 | 0.415 | - |
5,6-Dimethylbenzimidazole | pH 9.0, 37°C, wild-type | Sporomusa ovata |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.21 | Sporomusa ovata | F6MZ55 and F6MZ56 | subunit ArsA: F6MZ55, subunit ArsB: F6MZ56 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.21 | 0.0000007 | - |
substrate p-cresol, pH 9.0, 37°C, mutant M87Q/I321S | Sporomusa ovata |
2.4.2.21 | 0.0000024 | - |
substrate 5,6-dimethylbenzimidazole, pH 9.0, 37°C, mutant M87Q/I321S | Sporomusa ovata |
2.4.2.21 | 0.0000083 | - |
substrate p-cresol, pH 9.0, 37°C, mutant I321S | Sporomusa ovata |
2.4.2.21 | 0.0000142 | - |
substrate p-cresol, pH 9.0, 37°C, mutant M87Q | Sporomusa ovata |
2.4.2.21 | 0.0000167 | - |
substrate 5,6-dimethylbenzimidazole, pH 9.0, 37°C, mutant I321S | Sporomusa ovata |
2.4.2.21 | 0.0000376 | - |
substrate p-cresol, pH 9.0, 37°C, wild-type | Sporomusa ovata |
2.4.2.21 | 0.00016 | - |
substrate 5,6-dimethylbenzimidazole, pH 9.0, 37°C, wild-type | Sporomusa ovata |
2.4.2.21 | 0.000192 | - |
substrate 5,6-dimethylbenzimidazole, pH 9.0, 37°C, mutant M87Q | Sporomusa ovata |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.21 | 5,6-dimethylbenzimidazole + beta-nicotinate D-ribonucleotide | - |
Sporomusa ovata | nicotinate + N1-(5-phospho-alpha-D-ribosyl)-5,6-dimethylbenzimidazole | - |
? | |
2.4.2.21 | additional information | phloroglucinol is identified from an additive screen as a compound that dramatically improves the size, stability, and diffraction properties of the crystals. Subsequent structural studies show that phloroglucinol binds in the active site of ArsA | Sporomusa ovata | ? | - |
? | |
2.4.2.21 | p-cresol + beta-nicotinate D-ribonucleotide | - |
Sporomusa ovata | nicotinate + 4-hydroxyphenyl alpha-D-ribose 5-phosphate | - |
? | |
2.4.2.21 | phenol + beta-nicotinate D-ribonucleotide | - |
Sporomusa ovata | nicotinate + phenyl alpha-D-ribose 5-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.21 | heterodimer | ArsA and ArsB. Only the active site in ArsA is occupied by substrate. Analysis of the ArsB active site identified an arginyl side chain that may block access to the site | Sporomusa ovata |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.21 | ArsAB | - |
Sporomusa ovata |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.21 | 37 | - |
assay at | Sporomusa ovata |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.21 | 0.4 | - |
phenol | pH 9.0, 37°C, wild-type | Sporomusa ovata | |
2.4.2.21 | 1.6 | - |
5,6-Dimethylbenzimidazole | pH 9.0, 37°C, wild-type | Sporomusa ovata |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.21 | 9 | - |
assay at | Sporomusa ovata |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.21 | 3.9 | - |
5,6-Dimethylbenzimidazole | pH 9.0, 37°C, wild-type | Sporomusa ovata | |
2.4.2.21 | 8 | - |
phenol | pH 9.0, 37°C, wild-type | Sporomusa ovata |