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Literature summary extracted from

  • Yanai, H.; Doi, K.; Ohshima, T.
    Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase (2009), Appl. Environ. Microbiol., 75, 1758-1763.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expression in Escherichia coli Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zn2+ the enzyme likely contains 2 Zn2+ Sulfurisphaera tokodaii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 37477
-
 4 * 37477, calculeted from sequence Sulfurisphaera tokodaii
1.1.1.1 38000
-
 4 * 38000, SDS-PAGE Sulfurisphaera tokodaii
1.1.1.1 133000
-
 gel filtration Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Sulfurisphaera tokodaii F9VMI9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 1.14
-
 pH 10.5, 50°C Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1-hexanol + NAD+
-
 Sulfurisphaera tokodaii hexanal + NADH + H+
-
 ?
1.1.1.1 benzaldehyde + NADH
-
 Sulfurisphaera tokodaii benzyl alcohol + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 homotetramer 4 * 38000, SDS-PAGE Sulfurisphaera tokodaii
1.1.1.1 homotetramer 4 * 37477, calculeted from sequence Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 NAD-dependent medium-chain ADH
-
 Sulfurisphaera tokodaii
1.1.1.1 ST0053
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 50
-
 assay at Sulfurisphaera tokodaii
1.1.1.1 85
-
-
 Sulfurisphaera tokodaii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1 70 95 70°C: about 65% of maximal activity, 95°C: about 80% of maximal activity Sulfurisphaera tokodaii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 70
-
 10 min, stable. 120 min, 50% loss of activity Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 7
-
 reduction of benzaldehyde Sulfurisphaera tokodaii
1.1.1.1 10.5
-
 oxidation of 1-hexanol Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 5.5 7.5 pH 5.5: about 35% of maximal activity, pH 7.5: about 55% of maximal activity, reduction of benzaldehyde Sulfurisphaera tokodaii
1.1.1.1 7.5 10.8 pH 7.5: about 35% of maximal activity, pH 10.8: about 50% of maximal activity, oxidation of 1-hexanol Sulfurisphaera tokodaii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.1 1 2 30 min, 50°C, 50% loss of activity Sulfurisphaera tokodaii
1.1.1.1 3.3
-
 30 min, 50°C, 50% loss of activity Sulfurisphaera tokodaii
1.1.1.1 4 10 30 min, 50°C, no loss of activity Sulfurisphaera tokodaii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Sulfurisphaera tokodaii
1.1.1.1 NADH
-
 Sulfurisphaera tokodaii