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Literature summary extracted from

  • Kolvenbach, B.A.; Lenz, M.; Benndorf, D.; Rapp, E.; Fousek, J.; Vlcek, C.; Schaeffer, A.; Gabriel, F.L.; Kohler, H.P.; Corvini, P.F.
    Purification and characterization of hydroquinone dioxygenase from Sphingomonas sp. strain TTNP3 (2011), AMB Express, 1, 08.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.66 3,4-dihydroxybenzoate 0.2 mM, 94% inhibition; 0.2 mM, 94% inhibition Sphingomonas sp.
1.13.11.66 4-coumaric acid 0.2 mM, 97% inhibition; 0.2 mM, 97% inhibition Sphingomonas sp.
1.13.11.66 4-hydroxybenzoate 0.2 mM, 46% inhibition; 0.2 mM, 46% inhibition Sphingomonas sp.
1.13.11.66 caffeic acid 0.2 mM, 98% inhibition; 0.2 mM, 98% inhibition Sphingomonas sp.
1.13.11.66 catechol 0.2 mM, 93% inhibition; 0.2 mM, 93% inhibition Sphingomonas sp.
1.13.11.66 phenol 0.2 mM, 98% inhibition; 0.2 mM, 98% inhibition Sphingomonas sp.
1.13.11.66 resorcinol 0.2 mM, 99% inhibition; 0.2 mM, 99% inhibition Sphingomonas sp.
1.13.11.66 vanillate 0.2 mM, 86% inhibition; 0.2 mM, 86% inhibition Sphingomonas sp.
1.13.11.66 Vanillyl alcohol 0.2 mM, 62% inhibition; 0.2 mM, 62% inhibition Sphingomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.66 0.0022
-
 hydroquinone pH 7.0, 25°C Sphingomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.66 Iron 1.4 mol of Fe2+ per mol of enzyme Sphingomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.66 19000
-
 2 * 38000, large subunit, + 2 * 19000, small subunit Sphingomonas sp.
1.13.11.66 38000
-
 2 * 38000, large subunit, + 2 * 19000, small subunit Sphingomonas sp.
1.13.11.66 120000
-
 gel filtration Sphingomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.66 Sphingomonas sp. F8TW82 small subunit
-
1.13.11.66 Sphingomonas sp. F8TW83 large subunit
-
1.13.11.66 Sphingomonas sp. TTNP3 F8TW82 small subunit
-
1.13.11.66 Sphingomonas sp. TTNP3 F8TW83 large subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.66
-
 Sphingomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.66 6.1
-
 pH 7.0, 25°C Sphingomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.66 2-(1-methyl1-octyl)-hydroquinone + O2 less than 2% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
1.13.11.66 2-(1-methyl1-octyl)-hydroquinone + O2 less than 2% of the activity with hydroquinone Sphingomonas sp. TTNP3 ?
-
 ?
1.13.11.66 2-chlorohydroquinone + O2 29% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
1.13.11.66 2-chlorohydroquinone + O2 29% of the activity with hydroquinone Sphingomonas sp. TTNP3 ?
-
 ?
1.13.11.66 2-ethylhydroquinone + O2 83% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
1.13.11.66 2-ethylhydroquinone + O2 83% of the activity with hydroquinone Sphingomonas sp. TTNP3 ?
-
 ?
1.13.11.66 2-hexylhydroquinone + O2 less than 2% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
1.13.11.66 2-hexylhydroquinone + O2 less than 2% of the activity with hydroquinone Sphingomonas sp. TTNP3 ?
-
 ?
1.13.11.66 2-methoxyhydroquinone + O2 59% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
1.13.11.66 2-methylhydroquinone + O2 139% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
1.13.11.66 2-pentylhydroquinone + O2 19% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
1.13.11.66 2-propylhydroquinone + O2 23% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
1.13.11.66 2-tert-butylhydroquinone + O2 5% of the activity with hydroquinone Sphingomonas sp. ?
-
 ?
1.13.11.66 hydroquinone + O2 consumption of an equimolar amount of molecular oxygen Sphingomonas sp. 4-hydroxymuconic acid semialdehyde
-
 ?
1.13.11.66 additional information enzyme catalyzes the ring fission of hydroquinone to 4-hydroxymuconic semialdehyde and the degradation of chlorinated and several alkylated hydroquinones Sphingomonas sp. ?
-
 ?
1.13.11.66 additional information enzyme catalyzes the ring fission of hydroquinone to 4-hydroxymuconic semialdehyde and the degradation of chlorinated and several alkylated hydroquinones Sphingomonas sp. TTNP3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.66 tetramer 2 * 38000, large subunit, + 2 * 19000, small subunit Sphingomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.66 13.5
-
 hydroquinone pH 7.0, 25°C Sphingomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.66 7 8
-
 Sphingomonas sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.66 6136
-
 hydroquinone pH 7.0, 25°C Sphingomonas sp.