EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.61 | recombinant carboxy-terminal domain is purified after expression in Escherichia coli | Nostoc sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.11.61 | A162G | the recombinant carboxy-terminal domain of the wild-type enzyme and of mutant enzyme A162G produce primarily (9R,10EZ,12Z)-9-hydroperoxy-10,12-octadecadienoate from linoleate. The stereochemistry of the hydroperoxide is almost exclusively R (93-94%) | Nostoc sp. |
1.13.11.61 | A162I | the recombinant carboxy-terminal domain of the mutant enzyme A162I produces almost exlusively (13S)-hydroperoxy octadecadienoic acid (90%) | Nostoc sp. |
1.13.11.61 | A162V | the recombinant carboxy-terminal domain of the mutant enzyme A162V converts linoleate primarily to (13S)-hydroperoxy octadecadienoic acid (64%) and to a lesser extent to (9R,10EZ,12Z)-9-hydroperoxy-10,12-octadecadienoate (36%) | Nostoc sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.61 | 0.0044 | - |
linoleate | pH 7.5, 22°C, wild-type enzyme | Nostoc sp. | |
1.13.11.61 | 0.005 | - |
linoleate | pH 7.5, 22°C, mutant enzyme A162V | Nostoc sp. | |
1.13.11.61 | 0.0056 | - |
linoleate | pH 7.5, 22°C, mutant enzyme A162I | Nostoc sp. | |
1.13.11.61 | 0.0089 | - |
linoleate | pH 7.5, 22°C, mutant enzyme A162G | Nostoc sp. | |
1.13.11.61 | 0.026 | - |
alpha-linolenate | pH 7.5, 22°C, wild-type enzyme | Nostoc sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.61 | Nostoc sp. | - |
- |
- |
1.13.11.61 | Nostoc sp. PCC 7120 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.61 | recombinant carboxy-terminal domain is purified after expression in Escherichia coli | Nostoc sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.61 | alpha-linolenate + O2 | i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate | Nostoc sp. | (9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | - |
? | |
1.13.11.61 | alpha-linolenate + O2 | i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate | Nostoc sp. PCC 7120 | (9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | - |
? | |
1.13.11.61 | linoleate + O2 | i.e. (9Z,12Z)-octadeca-9,12-dienoate | Nostoc sp. | (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | configuration of the 9-hydroperoxide is primarily R (93%). (13S)-Hydroperoxy octadecadienoic acid is detected in small amounts as by-product | ? | |
1.13.11.61 | linoleate + O2 | i.e. (9Z,12Z)-octadeca-9,12-dienoate | Nostoc sp. PCC 7120 | (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | configuration of the 9-hydroperoxide is primarily R (93%). (13S)-Hydroperoxy octadecadienoic acid is detected in small amounts as by-product | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.61 | 9R-lipoxygenase | - |
Nostoc sp. |
1.13.11.61 | NspLOX | - |
Nostoc sp. |