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Literature summary extracted from

  • Palmen, T.G.; Nieveler, J.; Froelich, B.; Treffenfeldt, W.; Pohl, M.; Buechs, J.
    Physiological relation between respiration activity and heterologous expression of selected benzoylformate decarboxylase variants in Escherichia coli (2010), Microb. Cell Fact., 9, 76.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.7 expressed in Escherichia coli Escherichia coli SG13009 pKK233-2 Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.7 H281A mutant with high cofactor binding affinity Pseudomonas putida
4.1.1.7 L476H mutant with low cofactor binding affinity Pseudomonas putida
4.1.1.7 L476P mutant with low cofactor binding affinity Pseudomonas putida
4.1.1.7 L476P/S181T mutant with low cofactor binding affinity Pseudomonas putida
4.1.1.7 L476Q the mutant has an intermediate cofactor binding strength Pseudomonas putida
4.1.1.7 S181T mutant with high cofactor binding affinity Pseudomonas putida

General Stability

EC Number General Stability Organism
4.1.1.7 the wild type BFD has about 100% enzyme activity after 24 h incubation in 50 mM KPi-buffer without thiamine diphosphate Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7 Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 benzaldehyde + acetaldehyde
-
 Pseudomonas putida 2-hydroxypropiophenone
-
 ?
4.1.1.7 benzoylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.7 BFD
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.7 thiamine diphosphate
-
 Pseudomonas putida