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Literature summary extracted from

  • Tuekel, S.; Yildirim, D.; Alagoez, D.; Alptekin, O.; Yuecebilgi, G.; Bilgin, R.
    Partial purification and immobilization of a new (R)-hydroxynitrile lyase from seeds of Prunus pseudoarmeniaca (2010), J. Mol. Catal. B, 66, 161-165.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.1.2.10 both of immobilized enzymes (Eupergit C and Eupergit C250L) are used repeatedly 20times and the residual activities are about 97% of their initial activities Prunus pseudoarmeniaca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.10 1.03
-
 (R)-mandelonitrile enzyme immobilized onto Eupergit C 250L, in 50 mM citrate buffer, pH 6.0, 25°C Prunus pseudoarmeniaca
4.1.2.10 1.6
-
 (R)-mandelonitrile enzyme immobilized onto Eupergit C, in 50 mM citrate buffer, pH 6.0, 25°C Prunus pseudoarmeniaca
4.1.2.10 2.23
-
 (R)-mandelonitrile free enzyme, in 50 mM citrate buffer, pH 6.0, 25°C Prunus pseudoarmeniaca

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Prunus pseudoarmeniaca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10 ammonium sulfate precipitation Prunus pseudoarmeniaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.10 seed
-
 Prunus pseudoarmeniaca
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-mandelonitrile
-
 Prunus pseudoarmeniaca cyanide + benzaldehyde
-
 r
4.1.2.10 cyanide + benzaldehyde
-
 Prunus pseudoarmeniaca (R)-mandelonitrile
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 (R)-hydroxynitrile lyase
-
 Prunus pseudoarmeniaca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.10 25
-
 free and immobilized enzyme Prunus pseudoarmeniaca

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.2.10 50
-
 free enzyme retains about 57% of its maximum activity at 50°C whereas immobilized enzymes onto Eupergit C and Eupergit C 250 L retain about 75 and 82% of their maximum activities, respectively, at 50°C Prunus pseudoarmeniaca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.10 5 20 free and immobilized enzyme preparations show optimal carboligation activity at 5°C. As the temperature is increased from 5 to 20°C, the yields decrease, however enantiomeric excess values (99%) unchange Prunus pseudoarmeniaca
4.1.2.10 25 50 the half lives of free enzyme at 25 and 50°C are 49.9 and 30.5 h, respectively and these correspondingly are 96.3 and 43.6 h for immobilized enzyme onto Eupergit C, and 138.6 and 50.2 h for immobilized enzyme onto Eupergit C 250 L Prunus pseudoarmeniaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 6
-
 optimum pH for free and immobilized enzyme Prunus pseudoarmeniaca

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.10 4
-
 increasing the pH value above 4.0, the yield and ee are dramatically decreased due to the spontaneous chemical decomposition of the product Prunus pseudoarmeniaca