EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.7 | expressed in Escherichia coli SG13009 cells | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.7 | A460I | the mutant exhibits an increased (R)-2-hydroxypropiophenone selectivity | Pseudomonas putida |
4.1.1.7 | A460I/F464I | the mutant exhibits an increased (R)-2-hydroxypropiophenone selectivity | Pseudomonas putida |
4.1.1.7 | F464I | the mutant exhibits an increased (R)-2-hydroxypropiophenone selectivity | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.7 | Mg2+ | required | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.7 | benzoylformate | Pseudomonas putida | - |
benzaldehyde + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.7 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.7 | Q-Sepharose column chromatography and Superdex 200 gel filtration | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.7 | benzaldehyde + acetaldehyde | reactions performed at high benzaldehyde concentrations and at high hydrostatic pressures show an increase in (R)-2-hydroxypropiophenone ((R)-2-HPP) formation catalyzed by BFD variants F464I, A460I, and A460I/F464I. For BFD mutant A460I/F464I there is an increase in the ee of (R)-2-HPP up to 80%, whereas at atmospheric conditions this variant synthesizes (R)-2-HPP with an ee of only 50%. Alkaline conditions (up to pH 8.5) and high hydrostatic pressures result in an increase of (R)-2-HPP synthesis, especially in the case of variant A460I and F464I | Pseudomonas putida | (R)-2-hydroxypropiophenone | - |
? | |
4.1.1.7 | benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.7 | homotetramer | - |
Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.7 | BFD | - |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.7 | thiamine diphosphate | dependent on | Pseudomonas putida |