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Literature summary extracted from

  • Kara, S.; Long, W.S.; Berheide, M.; Peper, S.; Niemeyer, B.; Liese, A.
    Influence of reaction conditions on the enantioselectivity of biocatalyzed C-C bond formations under high pressure conditions (2011), J. Biotechnol., 152, 87-92.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.7 expressed in Escherichia coli SG13009 cells Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.7 A460I the mutant exhibits an increased (R)-2-hydroxypropiophenone selectivity Pseudomonas putida
4.1.1.7 A460I/F464I the mutant exhibits an increased (R)-2-hydroxypropiophenone selectivity Pseudomonas putida
4.1.1.7 F464I the mutant exhibits an increased (R)-2-hydroxypropiophenone selectivity Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.7 Mg2+ required Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.7 benzoylformate Pseudomonas putida
-
 benzaldehyde + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7 Q-Sepharose column chromatography and Superdex 200 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 benzaldehyde + acetaldehyde reactions performed at high benzaldehyde concentrations and at high hydrostatic pressures show an increase in (R)-2-hydroxypropiophenone ((R)-2-HPP) formation catalyzed by BFD variants F464I, A460I, and A460I/F464I. For BFD mutant A460I/F464I there is an increase in the ee of (R)-2-HPP up to 80%, whereas at atmospheric conditions this variant synthesizes (R)-2-HPP with an ee of only 50%. Alkaline conditions (up to pH 8.5) and high hydrostatic pressures result in an increase of (R)-2-HPP synthesis, especially in the case of variant A460I and F464I Pseudomonas putida (R)-2-hydroxypropiophenone
-
 ?
4.1.1.7 benzoylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.7 homotetramer
-
 Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
4.1.1.7 BFD
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.7 thiamine diphosphate dependent on Pseudomonas putida