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Literature summary extracted from

  • Kurtovic, I.; Marshall, S.N.; Zhao, X.; Simpson, B.K.
    Purification and properties of digestive lipases from Chinook salmon (Oncorhynchus tshawytscha) and New Zealand hoki (Macruronus novaezelandiae) (2010), Fish Physiol. Biochem., 36, 1041-1060.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 Colipase 113% activity at 0.01 mM Macruronus novaezelandiae
3.1.1.3 additional information not influenced by colipase and potassium iodide Oncorhynchus tshawytscha
3.1.1.3 sodium cholate absolutely necessary both for lipase stability in the gel and for optimum activity, 5 mM improves the lipase activity approximately 100fold Oncorhynchus tshawytscha
3.1.1.3 taurocholate absolutely necessary both for lipase stability in the gel and for optimum activity, 10 mM improves the lipase activity approximately 130fold Oncorhynchus tshawytscha

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Fe2+ 7% residual activity at 10 mM Macruronus novaezelandiae
3.1.1.3 Fe2+ 14% residual activity at 10 mM Oncorhynchus tshawytscha
3.1.1.3 Hg2+ complete inhibition at 1 mM Macruronus novaezelandiae
3.1.1.3 Hg2+ complete inhibition at 1 mM Oncorhynchus tshawytscha
3.1.1.3 Mg2+ 86% residual activity at 1 mM Macruronus novaezelandiae
3.1.1.3 Mg2+ 80% residual activity at 1 mM Oncorhynchus tshawytscha
3.1.1.3 NaN3 78% residual activity at 10 mM Macruronus novaezelandiae
3.1.1.3 NaN3 75% residual activity at 1 mM Oncorhynchus tshawytscha
3.1.1.3 paraoxon
-
 Macruronus novaezelandiae
3.1.1.3 paraoxon
-
 Oncorhynchus tshawytscha
3.1.1.3 phenylmethylsulfonyl fluoride 54% residual activity at 5 mM Macruronus novaezelandiae
3.1.1.3 phenylmethylsulfonyl fluoride 36% residual activity at 5 mM Oncorhynchus tshawytscha
3.1.1.3 Triton X-100 complete inhibition at 10% (v/v) Macruronus novaezelandiae
3.1.1.3 Triton X-100 complete inhibition at 10% (v/v) Oncorhynchus tshawytscha
3.1.1.3 Tween 80 20% residual activity at 10% (v/v) Macruronus novaezelandiae
3.1.1.3 Tween 80 33% residual activity at 10% (v/v) Oncorhynchus tshawytscha
3.1.1.3 Zn2+ 49% residual activity at 10 mM Macruronus novaezelandiae
3.1.1.3 Zn2+ 51% residual activity at 10 mM Oncorhynchus tshawytscha

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 0.068
-
 4-nitrophenyl caprate pH 8.0, 30°C Macruronus novaezelandiae
3.1.1.3 0.078
-
 4-nitrophenyl caprate pH 8.0, 30°C Oncorhynchus tshawytscha

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ absolutely necessary both for lipase stability in the gel and for optimum activity Macruronus novaezelandiae
3.1.1.3 Ca2+ absolutely necessary both for lipase stability in the gel and for optimum activity, improves the lipase activity approximately 100fold Oncorhynchus tshawytscha
3.1.1.3 Mn2+ 113% activity at 10 mM Macruronus novaezelandiae
3.1.1.3 Mn2+ 143% activity at 10 mM Oncorhynchus tshawytscha

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 45000
-
 x * 45000, calculated from SDS-PAGE Macruronus novaezelandiae
3.1.1.3 54900
-
 x * 54900, final enzyme form, calculated from SDS-PAGE Oncorhynchus tshawytscha
3.1.1.3 79600
-
 x * 79600, uncleaved enzyme form, calculated from SDS-PAGE Oncorhynchus tshawytscha

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 Acetone complete inhibition at 50% (v/v) Oncorhynchus tshawytscha
3.1.1.3 Acetone complete inhibition at 50% (v/v) Macruronus novaezelandiae
3.1.1.3 hexane 82% residual activity at 100% (v/v) Oncorhynchus tshawytscha
3.1.1.3 hexane 90% residual activity at 100% (v/v) Macruronus novaezelandiae
3.1.1.3 isooctane 58% residual activity at 100% (v/v) Oncorhynchus tshawytscha
3.1.1.3 isooctane 71% residual activity at 100% (v/v) Macruronus novaezelandiae
3.1.1.3 isopropanol 23% residual activity at 50% (v/v) Oncorhynchus tshawytscha
3.1.1.3 isopropanol 36% residual activity at 50% (v/v) Macruronus novaezelandiae
3.1.1.3 Methanol complete inhibition at 50% (v/v) Oncorhynchus tshawytscha
3.1.1.3 Methanol complete inhibition at 50% (v/v) Macruronus novaezelandiae
3.1.1.3 n-heptane 103% activity at 100% (v/v) Macruronus novaezelandiae
3.1.1.3 n-heptane 95% residual activity at 100% (v/v) Oncorhynchus tshawytscha

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Macruronus novaezelandiae
-
 New Zealand hoki
-
3.1.1.3 Oncorhynchus tshawytscha
-
 Chinook salmon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 fractional precipitation with polyethylene glycol 1000, cholate-Affi-Gel 102 affinity chromatography, and Sephacryl S-300 gel filtration Oncorhynchus tshawytscha
3.1.1.3 fractional precipitation with polyethylene glycol 1000, cholate-Affi-Gel 102 affinity chromatography, and Sephacryl S-300 gel filtration Macruronus novaezelandiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 pyloric cecum
-
 Oncorhynchus tshawytscha
-
3.1.1.3 pyloric cecum
-
 Macruronus novaezelandiae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 0.36
-
 crude extract, pH 8.0, 30°C Oncorhynchus tshawytscha
3.1.1.3 4.8
-
 after 14fold purification pH 8.0, 30°C Oncorhynchus tshawytscha
3.1.1.3 4.8
-
 after 14fold purification pH 8.0, 30°C Macruronus novaezelandiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-methylumbelliferyl butyrate + H2O
-
 Oncorhynchus tshawytscha 4-methylumbelliferone + butyrate
-
 ?
3.1.1.3 4-methylumbelliferyl butyrate + H2O
-
 Macruronus novaezelandiae 4-methylumbelliferone + butyrate
-
 ?
3.1.1.3 4-methylumbelliferyl oleate + H2O
-
 Oncorhynchus tshawytscha 4-methylumbelliferone + oleate
-
 ?
3.1.1.3 4-methylumbelliferyl oleate + H2O
-
 Macruronus novaezelandiae 4-methylumbelliferone + oleate
-
 ?
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Oncorhynchus tshawytscha 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl acetate + H2O
-
 Macruronus novaezelandiae 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl caprate + H2O best substrate Oncorhynchus tshawytscha 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl caprate + H2O best substrate Macruronus novaezelandiae 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl caprate + H2O highest lipase activity is obtained with 0.25 mM of the substrate Oncorhynchus tshawytscha 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl caprate + H2O highest lipase activity is obtained with 0.25 mM of the substrate Macruronus novaezelandiae 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O highest lipase activity is obtained with 0.25 mM of the substrate Oncorhynchus tshawytscha 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O highest lipase activity is obtained with 0.25 mM of the substrate Macruronus novaezelandiae 4-nitrophenol + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 45000, calculated from SDS-PAGE Macruronus novaezelandiae
3.1.1.3 ? x * 54900, final enzyme form, calculated from SDS-PAGE Oncorhynchus tshawytscha
3.1.1.3 ? x * 79600, uncleaved enzyme form, calculated from SDS-PAGE Oncorhynchus tshawytscha

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Oncorhynchus tshawytscha
3.1.1.3 lipase
-
 Macruronus novaezelandiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 35
-
-
 Oncorhynchus tshawytscha
3.1.1.3 35
-
-
 Macruronus novaezelandiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8 8.5
-
 Oncorhynchus tshawytscha
3.1.1.3 8 8.5
-
 Macruronus novaezelandiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.3 0.0028
-
 paraoxon pH 8.0, 30°C Oncorhynchus tshawytscha
3.1.1.3 0.00517
-
 paraoxon pH 8.0, 30°C Macruronus novaezelandiae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Oncorhynchus tshawytscha isoelectric focusing
-
 5.7
3.1.1.3 Macruronus novaezelandiae isoelectric focusing
-
 5.8