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Literature summary extracted from
- Effects of amines and aminoalcohols on bovine intestine alkaline phosphatase activity (2011), Enzyme Microb. Technol., 49, 171-176.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.1 | diethanolamine | highly activates BIALP. Km values increase with increasing concentrations of diethanolamine and N-methylethanolamine, but not triethanolamine | Bos taurus |  |
| 3.1.3.1 | N-Methylethanolamine | highly activates BIALP. Km values increase with increasing concentrations of diethanolamine and N-methylethanolamine, but not triethanolamine | Bos taurus | |
| 3.1.3.1 | triethanolamine | highly activates BIALP. Km values increase with increasing concentrations of diethanolamine and N-methylethanolamine, but not triethanolamine | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.1 | additional information | - |
4-nitrophenyl phosphate | the Km values increase with increasing concentrations of diethanolamine and N-methylethanolamine, but not triethanolamine | Bos taurus | |
| 3.1.3.1 | 0.07 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.05 M diethanolamine | Bos taurus | |
| 3.1.3.1 | 0.09 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.05 M methylamine | Bos taurus | |
| 3.1.3.1 | 0.18 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.2 M N-methylethanolamine | Bos taurus | |
| 3.1.3.1 | 0.24 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.1 M triethanolamine | Bos taurus | |
| 3.1.3.1 | 0.3 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.05 M ethanolamine | Bos taurus | |
| 3.1.3.1 | 0.3 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M ethanolamine | Bos taurus | |
| 3.1.3.1 | 0.31 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M triethanolamine | Bos taurus | |
| 3.1.3.1 | 0.35 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.02 M dimethylamine | Bos taurus | |
| 3.1.3.1 | 0.35 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.02 M ethylamine | Bos taurus | |
| 3.1.3.1 | 0.43 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M ethylamine | Bos taurus | |
| 3.1.3.1 | 0.62 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M methylamine | Bos taurus | |
| 3.1.3.1 | 0.65 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M dimethylamine | Bos taurus | |
| 3.1.3.1 | 0.83 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M diethanolamine | Bos taurus | |
| 3.1.3.1 | 2.5 | 3 | 4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M N-methylethanolamine | Bos taurus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.1 | Bos taurus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.1 | intestine | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.1 | 4-nitrophenyl phosphate + H2O | - |
Bos taurus | 4-nitrophenol + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.1 | alkaline phosphatase | - |
Bos taurus |
| 3.1.3.1 | BIALP | - |
Bos taurus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 20 | - |
assay at | Bos taurus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.1 | 140 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.05 M methylamine | Bos taurus | |
| 3.1.3.1 | 150 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.02 M dimethylamine | Bos taurus | |
| 3.1.3.1 | 190 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.05 M diethanolamine | Bos taurus | |
| 3.1.3.1 | 300 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M ethanolamine | Bos taurus | |
| 3.1.3.1 | 330 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M methylamine | Bos taurus | |
| 3.1.3.1 | 370 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M dimethylamine | Bos taurus | |
| 3.1.3.1 | 400 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M ethylamine | Bos taurus | |
| 3.1.3.1 | 450 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.02 M ethylamine | Bos taurus | |
| 3.1.3.1 | 500 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.2 M N-methylethanolamine | Bos taurus | |
| 3.1.3.1 | 610 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.05 M ethanolamine | Bos taurus | |
| 3.1.3.1 | 840 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 0.1 M triethanolamine | Bos taurus | |
| 3.1.3.1 | 1240 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M diethanolamine | Bos taurus | |
| 3.1.3.1 | 1450 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M triethanolamine | Bos taurus | |
| 3.1.3.1 | 2250 | - |
4-nitrophenyl phosphate | pH 9.8, 20°C, 1 M N-methylethanolamine | Bos taurus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 9.8 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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