EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.45 | expressed in Escherichia coli JM109 (pVLT33-ALD) and M15 (pREP4) cells | Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.45 | 3.58 | - |
Salicylaldehyde | at pH 7.0, 30°C | Pseudomonas fluorescens | |
4.1.2.45 | 8.98 | - |
benzaldehyde | at pH 7.0, 30°C | Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.2.45 | 38000 | - |
x * 38000, His-tagged recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.45 | Pseudomonas fluorescens | - |
- |
- |
4.1.2.45 | Pseudomonas fluorescens N3 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.45 | Co2+ Talon metal affinity resin column chromatography | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.45 | benzaldehyde + pyruvate | - |
Pseudomonas fluorescens | trans-benzylidenepyruvate | - |
? | |
4.1.2.45 | benzaldehyde + pyruvate | - |
Pseudomonas fluorescens N3 | trans-benzylidenepyruvate | - |
? | |
4.1.2.45 | salicylaldehyde + pyruvate | - |
Pseudomonas fluorescens | trans-o-hydroxybenzylidenepyruvate | - |
? | |
4.1.2.45 | salicylaldehyde + pyruvate | - |
Pseudomonas fluorescens N3 | trans-o-hydroxybenzylidenepyruvate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.2.45 | ? | x * 38000, His-tagged recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.45 | tHBP-HA | - |
Pseudomonas fluorescens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.45 | 40 | - |
at a fixed pH of 7.0 | Pseudomonas fluorescens |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.45 | 25 | 50 | - |
Pseudomonas fluorescens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.45 | 30 | 40 | after 30 min incubation, the decreases of the activity are 64% at 40°C and 24% at 35°C, while at 30°C, the activity remains unchanged. However, at 30°C, the decreases of the activity are 24% after 5 h and 54% after 24 h | Pseudomonas fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.45 | 7 | - |
- |
Pseudomonas fluorescens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.45 | 5.5 | 8.5 | - |
Pseudomonas fluorescens |