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Literature summary extracted from

  • Saar, J.; Kader, J.; Poralla, K.; Ourisson, G.
    Purification and some properties of the squalene-tetrahymanol cyclase from Tetrahymena thermophila (1991), Biochim. Biophys. Acta, 1075, 93-101.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.123 2,3-iminosqualene effective inhibitor, complete inhibition at 1 mM Tetrahymena thermophila
4.2.1.123 4,4,10beta-trimethyltransdecal-3beta-ol 25% inhibition at 40 mM Tetrahymena thermophila
4.2.1.123 4-chloromercuribenzenesulfonic acid 86% inhibition at 5 mM Tetrahymena thermophila
4.2.1.123 diethyldicarbonate complete inhibition at 5 mM Tetrahymena thermophila
4.2.1.123 Digitonin solubilization of the cyclase in 8 mg/ml digitonin inactivates the enzyme, its activity can be recovered by complementation of the assay buffer with octylthioglucoside above its critical micellar concentration Tetrahymena thermophila
4.2.1.123 Emulgene 911 solubilization of the cyclase in 1 mg/ml Emulgene 911 inactivates the enzyme Tetrahymena thermophila
4.2.1.123 additional information the cyclase is not influenced by EDTA concentrations up to 0.1 M, and lodoacetate is not inhibitory at 1 and 5 mM Tetrahymena thermophila
4.2.1.123 N,N-Dimethyldodecylamine-N-oxide effective inhibitor, complete inhibition at 1 mM Tetrahymena thermophila
4.2.1.123 Ro 43-8522 effective inhibitor, complete inhibition at 1 mM Tetrahymena thermophila
4.2.1.123 Triton X-100 strong inactivator Tetrahymena thermophila
4.2.1.123 Tween 80 solubilization of the cyclase in 30 mg/ml Tween 80 inactivates the enzyme, its activity can be recovered by complementation of the assay buffer with octylthioglucoside above its critical micellar concentration Tetrahymena thermophila

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.123 membrane
-
 Tetrahymena thermophila 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.123 72000
-
 SDS-PAGE Tetrahymena thermophila

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.2.1.123 CHAPS CHAPS effectively solubilizes the enzyme and maintains its activity Tetrahymena thermophila

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.123 Tetrahymena thermophila
-
-
-
4.2.1.123 Tetrahymena thermophila B4-1868 (ATCC 30382)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.123 DEAE-trisacryl column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography Tetrahymena thermophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.123 105
-
 enzyme from homogenate, pH 7.0, 30°C Tetrahymena thermophila
4.2.1.123 31280
-
 enzyme after 297fold purification, pH 7.0, 30°C Tetrahymena thermophila

Storage Stability

EC Number Storage Stability Organism
4.2.1.123 -80°C, whole cells and particulate material, 6 months, no loss of activity Tetrahymena thermophila
4.2.1.123 6°C, octylthioglucoside-solubilized enzyme, one week, almost complete loss of activity Tetrahymena thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.123 2 squalene + 2 H2O
-
 Tetrahymena thermophila tetrahymanol + diplopterol diplopterol amounts to less than 2% of the total products formed by enzymatic conversion ?
4.2.1.123 2 squalene + 2 H2O
-
 Tetrahymena thermophila B4-1868 (ATCC 30382) tetrahymanol + diplopterol diplopterol amounts to less than 2% of the total products formed by enzymatic conversion ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.123 squalene-tetrahymanol cyclase
-
 Tetrahymena thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.123 30
-
-
 Tetrahymena thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.123 50
-
 the enzyme is not active at 50°C Tetrahymena thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.123 7
-
-
 Tetrahymena thermophila

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.123 4 8.5 the enzyme is not active beyond the pH limits of pH 4.5 and 8.0 Tetrahymena thermophila

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.123 0.00003
-
 pH 7.0, 30°C Tetrahymena thermophila 2,3-iminosqualene
4.2.1.123 0.00004
-
 pH 7.0, 30°C Tetrahymena thermophila Ro 43-8522
4.2.1.123 0.00005
-
 pH 7.0, 30°C Tetrahymena thermophila N,N-Dimethyldodecylamine-N-oxide