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Literature summary extracted from

  • Li, X.; Li, Y.; Wei, D.; Li, P.; Wang, L.; Feng, L.
    Characterization of a broad-range aldehyde dehydrogenase involved in alkane degradation in Geobacillus thermodenitrificans NG80-2 (2010), Microbiol. Res., 165, 706-712.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.3 Triton X-100 2% (v/v) Triton X-100 enhances the enzyme activity by 270% Geobacillus thermodenitrificans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expressed in Escherichia coli BL21(DE3) cells Geobacillus thermodenitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 Co2+ complete inhibition at 1 mM Geobacillus thermodenitrificans
1.2.1.3 Cu2+ complete inhibition at 1 mM Geobacillus thermodenitrificans
1.2.1.3 Fe3+ complete inhibition at 1 mM Geobacillus thermodenitrificans
1.2.1.3 additional information EDTA has no effect on ALDH activity Geobacillus thermodenitrificans
1.2.1.3 Ni2+ complete inhibition at 1 mM Geobacillus thermodenitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.0066
-
 acetaldehyde in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C Geobacillus thermodenitrificans
1.2.1.3 0.389
-
 NAD+ in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C Geobacillus thermodenitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.3 Ca2+ 150% activity at 1 mM Geobacillus thermodenitrificans
1.2.1.3 K+ 118% activity at 1 mM Geobacillus thermodenitrificans
1.2.1.3 Mg2+ 180% activity at 1 mM Geobacillus thermodenitrificans
1.2.1.3 Mn2+ 140% activity at 1 mM Geobacillus thermodenitrificans
1.2.1.3 additional information 1 mM Na+ has no effect on the enzyme activity Geobacillus thermodenitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 56532
-
 4 * 56532, calculated from amino acid sequence Geobacillus thermodenitrificans
1.2.1.3 57000
-
 4 * 57000, SDS-PAGE Geobacillus thermodenitrificans
1.2.1.3 220000
-
 major band, gel filtration Geobacillus thermodenitrificans
1.2.1.3 440000
-
 minor band, gel filtration Geobacillus thermodenitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Geobacillus thermodenitrificans
-
-
-
1.2.1.3 Geobacillus thermodenitrificans NG80-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 chelating Sepharose column chromatography, gel filtration Geobacillus thermodenitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 2-chlorobenzaldehyde + NAD+ + H2O 28.35% activity compared to formaldehyde Geobacillus thermodenitrificans 2-chlorobenzoate + NADH + H+
-
 ?
1.2.1.3 2-phthalaldehyde + NAD+ + H2O 10.81% activity compared to formaldehyde Geobacillus thermodenitrificans phthalate + NADH + H+
-
 ?
1.2.1.3 acetaldehyde + NAD+ + H2O 77.68% activity compared to formaldehyde Geobacillus thermodenitrificans acetate + NADH + H+
-
 ?
1.2.1.3 acetaldehyde + NAD+ + H2O 77.68% activity compared to formaldehyde Geobacillus thermodenitrificans NG80-2 acetate + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O 72.38% activity compared to formaldehyde Geobacillus thermodenitrificans benzoate + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O 72.38% activity compared to formaldehyde Geobacillus thermodenitrificans NG80-2 benzoate + NADH + H+
-
 ?
1.2.1.3 butyraldehyde + NAD+ + H2O 71.65% activity compared to formaldehyde Geobacillus thermodenitrificans butyrate + NADH + H+
-
 ?
1.2.1.3 butyraldehyde + NAD+ + H2O 71.65% activity compared to formaldehyde Geobacillus thermodenitrificans NG80-2 butyrate + NADH + H+
-
 ?
1.2.1.3 decanaldehyde + NAD+ + H2O 50.3% activity compared to formaldehyde Geobacillus thermodenitrificans decanoate + NADH + H+
-
 ?
1.2.1.3 dodecanaldehyde + NAD+ + H2O 42.02% activity compared to formaldehyde Geobacillus thermodenitrificans dodecanoate + NADH + H+
-
 ?
1.2.1.3 eicosanoic aldehyde + NAD+ + H2O 8.79% activity compared to formaldehyde Geobacillus thermodenitrificans eicosanoate + NADH + H+
-
 ?
1.2.1.3 formaldehyde + NAD+ + H2O 100% activity Geobacillus thermodenitrificans formate + NADH + H+
-
 ?
1.2.1.3 hexadecanal + NAD+ + H2O 15.77% activity compared to formaldehyde Geobacillus thermodenitrificans hexadecanoate + NADH + H+
-
 ?
1.2.1.3 hexaldehyde + NAD+ + H2O 76.21% activity compared to formaldehyde Geobacillus thermodenitrificans hexanoate + NADH + H+
-
 ?
1.2.1.3 additional information no activity towards NADP+, tetracosaldehyde, 2-hydroxylbenzaldehyde, ethanol, hexadecanol, acetate, and hexadecanoic acid Geobacillus thermodenitrificans ?
-
 ?
1.2.1.3 additional information the ALDH has no reductive activity on acetate or hexadecanoic acid Geobacillus thermodenitrificans ?
-
 ?
1.2.1.3 additional information no activity towards NADP+, tetracosaldehyde, 2-hydroxylbenzaldehyde, ethanol, hexadecanol, acetate, and hexadecanoic acid Geobacillus thermodenitrificans NG80-2 ?
-
 ?
1.2.1.3 additional information the ALDH has no reductive activity on acetate or hexadecanoic acid Geobacillus thermodenitrificans NG80-2 ?
-
 ?
1.2.1.3 octadecanal + NAD+ + H2O 12% activity compared to formaldehyde Geobacillus thermodenitrificans octadecanoate + NADH + H+
-
 ?
1.2.1.3 octyl aldehyde + NAD+ + H2O 72.27% activity compared to formaldehyde Geobacillus thermodenitrificans octanoate + NADH + H+
-
 ?
1.2.1.3 phenylacetaldehyde + NAD+ + H2O 37.86% activity compared to formaldehyde Geobacillus thermodenitrificans phenylacetate + NADH + H+
-
 ?
1.2.1.3 tetradecyl aldehyde + NAD+ + H2O 11.71% activity compared to formaldehyde Geobacillus thermodenitrificans tetradecanoate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 homotetramer 4 * 57000, SDS-PAGE Geobacillus thermodenitrificans
1.2.1.3 homotetramer 4 * 56532, calculated from amino acid sequence Geobacillus thermodenitrificans

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 ALDH
-
 Geobacillus thermodenitrificans
1.2.1.3 irreversible NAD+-dependent aldehyde dehydrogenase
-
 Geobacillus thermodenitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.3 60
-
-
 Geobacillus thermodenitrificans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.3 40 70
-
 Geobacillus thermodenitrificans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.3 40 80 the purified enzyme retains 97%, 85%, 71%, and 56% of the original activity, respectively, after incubation at 40, 50, 60 and 70°C for 60 min. The enzyme is inactivated after incubation at 80°C Geobacillus thermodenitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 8
-
-
 Geobacillus thermodenitrificans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.3 6 8.8
-
 Geobacillus thermodenitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+ dependent on Geobacillus thermodenitrificans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.3 0.006
-
 NAD+ in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C Geobacillus thermodenitrificans
1.2.1.3 0.49
-
 acetaldehyde in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C Geobacillus thermodenitrificans