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Literature summary extracted from

  • Lavery, C.B.; Macinnis, M.C.; Macdonald, M.J.; Williams, J.B.; Spencer, C.A.; Burke, A.A.; Irwin, D.J.; D'Cunha, G.B.
    Purification of peroxidase from horseradish (Armoracia rusticana) roots (2010), J. Agric. Food Chem., 58, 8471-8476.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.11.1.7 the optimal buffer for the assay of purified HRP is 50 mM phosphate buffer (pH 7.5) Armoracia rusticana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 40000
-
 SDS-PAGE Armoracia rusticana

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Armoracia rusticana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 ultrasonication, ammonium sulfate salt precipitation, and phenyl Sepharose column chromatography Armoracia rusticana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.7 root
-
 Armoracia rusticana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.7 2.65
-
 crude enzyme, using guaiacol as substrate, in 50 mM phosphate buffer (pH 7.5), at 30°C Armoracia rusticana
1.11.1.7 772.3
-
 after 291fold purification, using guaiacol as substrate, in 50 mM phosphate buffer (pH 7.5), at 30°C Armoracia rusticana

Storage Stability

EC Number Storage Stability Organism
1.11.1.7 -20°C, 50 mM phosphate buffer (pH 7.5) containing 20% (v/v) glycerol, at least 6 months, less than 5% loss of activity Armoracia rusticana
1.11.1.7 0-2°C, 50 mM phosphate buffer (pH 7.5), 48-72 h, 40-45% loss of activity Armoracia rusticana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 2-chlorophenol + H2O2 optimal concentrations of 2-chlorophenol and H2O2 are 0.2 mM and 0.3 mM, respectively Armoracia rusticana ?
-
 r
1.11.1.7 guaiacol + H2O2 optimal concentrations of guaiacol and H2O2 are 0.5 mM and 0.3 mM, respectively Armoracia rusticana tetraguaiacol + H2O
-
 r
1.11.1.7 phenol + H2O2 optimal concentrations of phenol and H2O2 are 0.2 mM and 0.3 mM, respectively Armoracia rusticana ?
-
 r

Subunits

EC Number Subunits Comment Organism
1.11.1.7 monomer 1 * 40000, SDS-PAGE Armoracia rusticana

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 HRP
-
 Armoracia rusticana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.7 28
-
 optimum temperature using phenol or 2-chlorophenol as substrate Armoracia rusticana
1.11.1.7 30
-
 optimum temperature using guaiacol as substrate Armoracia rusticana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.11.1.7 42
-
 thermal denaturation of isolated HRP occurs at temperatures above 42°C, and the enzyme activity decreases rapidly Armoracia rusticana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.7 7
-
-
 Armoracia rusticana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.7 6 8 although peroxidase activity is comparable in the pH range of 6.0-7.5, significant decline in enzyme activity is observed when the pH is increased to above 8.0 Armoracia rusticana

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.7 heme
-
 Armoracia rusticana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.7 Armoracia rusticana isoelectric focusing
-
 8.8