EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.3 | expressed in in Sf9 insect cells and in Mus musculus (highest human ALDH1B1 mRNA expression is detected in liver and part of the small intestine, moderate levels in the testis, distal colon, lung, heart, and duodenum and the lowest levels are found in the kidney and stomach) | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.1.3 | E487K | the mutation results in poor binding affinity to cofactor NAD+ and about 90% loss of enzyme activity | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.3 | Disulfiram | at higher concentrations of propionaldehyde (0.5 and 1 mM), ALDH1B1 activity is inhibited by disulfiram, with the extent of inhibition being about 40% at 0.01 mM disulfiram | Homo sapiens | |
1.2.1.3 | additional information | at the lowest concentration of substrate tested (propionaldehyde 0.1 mM), ALDH1B1 is not affected by disulfiram | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.3 | 0.0004 | - |
hexanal | at 25°C, pH not specified in the publication | Homo sapiens | |
1.2.1.3 | 0.0008 | - |
n-nonanal | at 25°C, pH not specified in the publication | Homo sapiens | |
1.2.1.3 | 0.0036 | - |
NAD+ | at 25°C, pH not specified in the publication | Homo sapiens | |
1.2.1.3 | 0.014 | - |
propionaldehyde | at 25°C, pH not specified in the publication | Homo sapiens | |
1.2.1.3 | 0.05 | - |
benzaldehyde | at 25°C, pH not specified in the publication | Homo sapiens | |
1.2.1.3 | 0.055 | - |
acetaldehyde | at 25°C, pH not specified in the publication | Homo sapiens | |
1.2.1.3 | 0.466 | - |
malondialdehyde | at 25°C, pH not specified in the publication | Homo sapiens | |
1.2.1.3 | 3.383 | - |
4-hydroxy-2-nonenal | at 25°C, pH not specified in the publication | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.2.1.3 | cytoplasm | - |
Homo sapiens | 5737 | - |
1.2.1.3 | mitochondrion | - |
Homo sapiens | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 50000 | - |
recombinant enzyme, SDS-PAGE | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.3 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.3 | 5'-AMP-Sepharose 4B affinity column chromatography, gel filtration | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.3 | liver | - |
Homo sapiens | - |
1.2.1.3 | pancreas | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.3 | 4-hydroxy-2-nonenal + NAD+ + H2O | low affinity for substrate binding and poor catalytic efficiency | Homo sapiens | 4-hydroxy-2-nonenoate + NADH + H+ | - |
? | |
1.2.1.3 | acetaldehyde + NAD+ + H2O | - |
Homo sapiens | acetate + NADH + H+ | - |
? | |
1.2.1.3 | benzaldehyde + NAD+ + H2O | - |
Homo sapiens | benzoate + NADH + H+ | - |
? | |
1.2.1.3 | hexanal + NAD+ + H2O | - |
Homo sapiens | hexanoate + NADH + H+ | - |
? | |
1.2.1.3 | malondialdehyde + NAD+ + H2O | low affinity for substrate binding and poor catalytic efficiency | Homo sapiens | ? | - |
? | |
1.2.1.3 | additional information | no activity with NADP+ and unsaturated aldehydes like trans-2-hexenal, trans-2-nonenal, and trans-2 dodecenal | Homo sapiens | ? | - |
? | |
1.2.1.3 | additional information | the enzyme shows esterase activity with 4-nitrophenyl acetate | Homo sapiens | ? | - |
? | |
1.2.1.3 | n-nonanal + NAD+ + H2O | - |
Homo sapiens | n-nonanoate + NADH + H+ | - |
? | |
1.2.1.3 | propionaldehyde + NAD+ + H2O | - |
Homo sapiens | propionate + NADH | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.3 | aldehyde dehydrogenase 1B1 | isoform | Homo sapiens |
1.2.1.3 | Aldh1b1 | isoform | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.3 | NAD+ | isoform ALDH1B1 has an exclusive preference for NAD+ as the cofactor, no catalytic activity occurs when NADP+ is used | Homo sapiens |