EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.47 | benzaldehyde | strong competitive inhibitor, the half-life of HNL in 20 mM citrate-phosphate buffer (pH 5.0) in the presence of 10 mM of benzaldehyde is around 50 min compared to 450 min in the absence of benzaldehyde; strong inhibitor | Hevea brasiliensis | |
4.1.2.47 | additional information | (R)-2-nitro-1-phenylethanol and nitromethane have negligible inhibition effects on HNL activity | Hevea brasiliensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.47 | 2.6 | - |
(S)-2-nitro-1-phenylethanol | in 50 mM phosphate buffer pH 6.0, at 25°C | Hevea brasiliensis | |
4.1.2.47 | 2.6 | - |
(S)-2-nitro-1-phenylethanol | in 50 mM phosphate buffer pH 6.0, at 22°C | Hevea brasiliensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.47 | Hevea brasiliensis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.47 | (S)-2-nitro-1-phenylethanol | - |
Hevea brasiliensis | benzaldehyde + nitromethane | - |
r | |
4.1.2.47 | (S)-2-nitro-1-phenylethanol | besides the native cyanohydrins reaction, the enzyme also catalyzes the asymmetric reversible Henry reaction yielding (S)-beta-nitroalcohols with high enantiomeric excess. The catalyst productivity achieved during the resolution is 10times higher than that in the HNL-catalyzed synthesis of (S)-2-nitro-1-phenylethanol | Hevea brasiliensis | nitromethane + benzaldehyde + (R)-2-nitro-1-phenylethanol | - |
r | |
4.1.2.47 | (S)-mandelonitrile | - |
Hevea brasiliensis | HCN + benzaldehyde | - |
? | |
4.1.2.47 | cyanide + benzaldehyde | - |
Hevea brasiliensis | (S)-mandelonitrile | - |
r | |
4.1.2.47 | additional information | the enzyme catalyzes asymmetric cyanohydrin and Henry reactions. (R)-2-nitro-1-phenylethanol is not a substrate or HNL | Hevea brasiliensis | ? | - |
? | |
4.1.2.47 | nitromethane + benzaldehyde | - |
Hevea brasiliensis | (S)-2-nitro-1-phenylethanol | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.47 | EC 4.1.2.39 | formerly | Hevea brasiliensis |
4.1.2.47 | HNL | - |
Hevea brasiliensis |
4.1.2.47 | Hydroxynitrile lyase | - |
Hevea brasiliensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.47 | 7.5 | - |
the maximal activity of HNL in (S)-2-nitro-1-phenylethanol cleavage is at/above pH 7.5 | Hevea brasiliensis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.47 | 0.37 | - |
benzaldehyde | in 50 mM phosphate buffer pH 6.0, at 25°C | Hevea brasiliensis | |
4.1.2.47 | 0.37 | - |
benzaldehyde | in 50 mM phosphate buffer pH 6.0, at 22°C | Hevea brasiliensis |