Any feedback?
Literature summary extracted from
- Characterisation of lipoxygenase isoforms from olive callus cultures (2008), Phytochemistry, 69, 2532-2538.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.12 | 0.043 | - |
linoleic acid | 55fold purified enzyme, at pH 8.8, isoform 2 | Olea europaea |  |
| 1.13.11.12 | 0.08 | - |
linoleic acid | 48fold purified enzyme, at pH 8.8, isoform 1 | Olea europaea | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 95000 | - |
Western blotting and gel filtration | Olea europaea |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.12 | Olea europaea | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.12 | by ammonium sulphate precipitation and ion-exchange chromatography, 55fold with a yield of 5.6% | Olea europaea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.13.11.12 | callus | - |
Olea europaea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 10.2 | - |
crude extract, with linoleic acid as substrate | Olea europaea |
| 1.13.11.12 | 12 | - |
crude extract, with alpha-linolenic acid as substrate | Olea europaea |
| 1.13.11.12 | 474 | - |
55fold purified enzyme, with alpha-linolenic acid as substrate | Olea europaea |
| 1.13.11.12 | 564 | - |
55fold purified enzyme, with linoleic acid as substrate | Olea europaea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.12 | alpha-linolenic acid + O2 | - |
Olea europaea | ? | - |
? | |
| 1.13.11.12 | linoleic acid + O2 | - |
Olea europaea | (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.12 | LOX | - |
Olea europaea |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 8 | - |
for alpha-linolenic acid | Olea europaea |
| 1.13.11.12 | 8.8 | - |
for linoleic acid | Olea europaea |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 7.8 | 8.4 | at pH 7.8, ca. 50% of maximal activity, at pH 8.4, ca. 30% of maximal activity, substrate alpha-linoleic acid | Olea europaea |
| 1.13.11.12 | 8.5 | 8.9 | at pH 8.5, ca. 30% of maximal activity, at pH 8.9, ca. 80% of maximal activity, substrate linolenic acid | Olea europaea |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
