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Literature summary extracted from

  • Kurutsch, A.; Richter, M.; Brecht, V.; Sprenger, G.; Müller, M.
    MenD as a versatile catalyst for asymmetric synthesis (2009), J. Mol. Catal. B, 61, 56-66.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.9 expression in Escherichia coli BL21 Escherichia coli K-12

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.9 Escherichia coli K-12
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.9 gel permeation chromatography Escherichia coli K-12

Storage Stability

EC Number Storage Stability Organism
2.2.1.9 4°C, desalting buffer with 50 mM phosphate, 1 mM thiamine diphosphate and 2 mM MgCl2, pH 8.0, 4 weeks, no significant loss of activity Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.9 2,3-dihydroxybenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-(2,3-dihydroxyphenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 2-bromobenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-(2-bromophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 2-chlorobenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-(2-chlorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 2-fluorobenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-(2-fluorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 2-fluorobenzaldehyde + oxaloacetate assay at pH 8 Escherichia coli K-12 (1R)-1-(2-fluorophenyl)-1-hydroxypropan-2-one + 2 CO2
-
 ?
2.2.1.9 2-fluorobenzaldehyde + pyruvate assay at pH 8 Escherichia coli K-12 (1R)-1-(2-fluorophenyl)-1-hydroxypropan-2-one + CO2
-
 ?
2.2.1.9 2-iodobenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-hydroxy-5-(2-iodophenyl)-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 2-methylbenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-hydroxy-5-(2-methylphenyl)-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 3,4-dihydroxybenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 3-fluorobenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-(3-fluorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 3-iodobenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-hydroxy-5-(3-iodophenyl)-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 3-methoxybenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-hydroxy-5-(3-methoxyphenyl)-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 4-chlorbenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-(4-chlorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 4-fluorobenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-(4-fluorophenyl)-5-hydroxy-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 4-hydroxybenzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-hydroxy-5-(4-hydroxyphenyl)-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 acetaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-hydroxy-4-oxohexanoic acid + CO2
-
 ?
2.2.1.9 benzaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-hydroxy-4-oxo-5-phenylpentanoic acid + CO2
-
 ?
2.2.1.9 benzaldehyde + oxaloacetate assay at pH 8 Escherichia coli K-12 (1R)-1-hydroxy-1-phenylpropan-2-one + 2 CO2
-
 ?
2.2.1.9 benzaldehyde + pyruvate assay at pH 8 Escherichia coli K-12 (1R)-1-hydroxy-1-phenylpropan-2-one + 2 CO2
-
 ?
2.2.1.9 cyclohex-1-ene-1-carbaldehyde + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-cyclohex-1-en-1-yl-5-hydroxy-4-oxopentanoic acid + CO2
-
 ?
2.2.1.9 glyoxylate + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 5-hydroxy-4-oxopentanoic acid + 2 CO2
-
 ?
2.2.1.9 hexanal + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-hydroxy-4-oxodecanoic acid + CO2
-
 ?
2.2.1.9 isochorismate + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (1R,2S,5S,6S)-5-[(1-carboxyethenyl)oxy]-2-(3-carboxypropanoyl)-6-hydroxycyclohex-3-ene-1-carboxylic acid + CO2
-
 ?
2.2.1.9 pyruvate + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 4-hydroxy-5-oxohexanoic acid + 2 CO2
-
 ?
2.2.1.9 undec-10-enal + 2-oxoglutarate assay at pH 8 Escherichia coli K-12 (5R)-5-hydroxy-4-oxopentadec-14-enoic acid + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.9 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
-
 Escherichia coli K-12
2.2.1.9 MenD
-
 Escherichia coli K-12
2.2.1.9 SHCHC synthase
-
 Escherichia coli K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.9 8
-
 assay at Escherichia coli K-12

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.9 thiamine diphosphate
-
 Escherichia coli K-12