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Literature summary extracted from

  • Ueatrongchit, T.; Komeda, H.; Asano, Y.; H-Kittikun, A.
    Parameters influencing asymmetric synthesis of (R)-mandelonitrile by a novel (R)-hydroxynitrile lyase from Eriobotrya japonica (2009), J. Mol. Catal. B, 56, 208-214.
No PubMed abstract available

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.1.2.10 dibutyl ether EjHNL is very stable in ethyl acetate, diethyl ether, methyl-tert-butyl ether, diisopropyl ether, dibutyl ether and hexane for 12 h. The best solvent for the highest initial velocity and enantiomeric excess is diethyl ether with an optimum aqueous phase content of 50% (v/v) Rhaphiolepis bibas
4.1.2.10 diethyl ether EjHNL is very stable in ethyl acetate, diethyl ether, methyl-tert-butyl ether, diisopropyl ether, dibutyl ether and hexane for 12 h. The best solvent for the highest initial velocity and enantiomeric excess is diethyl ether with an optimum aqueous phase content of 50% (v/v) Rhaphiolepis bibas
4.1.2.10 diisopropyl ether EjHNL is very stable in ethyl acetate, diethyl ether, methyl-tert-butyl ether, diisopropyl ether, dibutyl ether and hexane for 12 h. The best solvent for the highest initial velocity and enantiomeric excess is diethyl ether with an optimum aqueous phase content of 50% (v/v) Rhaphiolepis bibas
4.1.2.10 Ethyl acetate EjHNL is very stable in ethyl acetate, diethyl ether, methyl-tert-butyl ether, diisopropyl ether, dibutyl ether and hexane for 12 h. The best solvent for the highest initial velocity and enantiomeric excess is diethyl ether with an optimum aqueous phase content of 50% (v/v) Rhaphiolepis bibas
4.1.2.10 hexane EjHNL is very stable in ethyl acetate, diethyl ether, methyl-tert-butyl ether, diisopropyl ether, dibutyl ether and hexane for 12 h. The best solvent for the highest initial velocity and enantiomeric excess is diethyl ether with an optimum aqueous phase content of 50% (v/v) Rhaphiolepis bibas
4.1.2.10 methyl-tert-butylether EjHNL is very stable in ethyl acetate, diethyl ether, methyl-tert-butyl ether, diisopropyl ether, dibutyl ether and hexane for 12 h. The best solvent for the highest initial velocity and enantiomeric excess is diethyl ether with an optimum aqueous phase content of 50% (v/v) Rhaphiolepis bibas

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Rhaphiolepis bibas
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.10 seed
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 Rhaphiolepis bibas
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 cyanide + benzaldehyde both pH and temperature have a large effect on the initial velocity and enantiomeric excess (e.e.) of the product, (R)-mandelonitrile. High enantiomeric purity of the product is observed at low pH and temperature because the non-enzymatic reaction producing racemates of mandelonitrile is almost suppressed. The optimum pH and temperature to obtain high enantiomeric excess are pH 4.0 and 10°C, respectively. The best solvent for the highest initial velocity and enantiomeric excess is diethyl ether with an optimum aqueous phase content of 50% (v/v). The initial reaction rate increases as the aqueous phase content rises, but when the content is more than 50%, a reduction of enantiomeric excess is observed. Increasing the concentration of the substrates accelerates the initial velocity, but causes a slight decrease in the e.e. of the product. Under the optimized conditions, the conversion and enantiomeric excess of (R)-mandelonitrile for 3 h are 40 and 99%, respectively Rhaphiolepis bibas (R)-mandelonitrile
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 ?
4.1.2.10 additional information the enzyme also catalyzes transcyanation of benzaldehyde and acetone cyanohydrin to (R)-mandelonitrile Rhaphiolepis bibas ?
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Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 (R)-hydroxynitrile lyase
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 Rhaphiolepis bibas
4.1.2.10 EjHNL
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 Rhaphiolepis bibas

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.10 10
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 selected as the optimum temperature for the reaction to avoid the non-enzymatic reaction producing racemates of mandelonitrile Rhaphiolepis bibas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 4
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 selected as the optimum temperature for the reaction to avoid the non-enzymatic reaction producing racemates of mandelonitrile Rhaphiolepis bibas