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Literature summary extracted from

  • Hattori, K.; Motohashi, N.; Kobayashi, I.; Tohya, T.; Oikawa, M.; Tamura, H.O.
    Cloning, expression, and characterization of cytosolic sulfotransferase isozymes from Drosophila melanogaster (2008), Biosci. Biotechnol. Biochem., 72, 540-547.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.1 isoforms ST1, ST3, and ST4 are expressed in Escherichia coli BL21(DE3) cells Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 0.0012
-
 3'-phosphoadenylyl sulfate isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
2.8.2.1 0.0021
-
 3'-phosphoadenylyl sulfate isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
2.8.2.1 0.024
-
 3'-phosphoadenylyl sulfate isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
2.8.2.1 0.037
-
 4-nitrophenol isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
2.8.2.1 0.24
-
 4-nitrophenol isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
2.8.2.1 1.3
-
 4-nitrophenol isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.1 Mg2+ MgCl2 (10 mM) enhances isoform ST3 activity by 50%, whereas no effects of these cations are observed for isoforms ST1 and ST4 Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.1 37000
-
 isoform ST2, estimated from amino acid sequence Drosophila melanogaster
2.8.2.1 37000
-
 isoform ST3, estimated from amino acid sequence Drosophila melanogaster
2.8.2.1 39000
-
 isoform ST1, estimated from amino acid sequence Drosophila melanogaster
2.8.2.1 40000
-
 isoform ST1, gel filtration Drosophila melanogaster
2.8.2.1 40000
-
 isoform ST3, gel filtration Drosophila melanogaster
2.8.2.1 41000
-
 isoform ST4, estimated from amino acid sequence Drosophila melanogaster
2.8.2.1 52000
-
 isoform ST4, gel filtration Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + 1-naphthol Drosophila melanogaster isoform ST4 shows a preference toward 1-naphtol adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + 4-nitrophenol Drosophila melanogaster isoform ST3 shows higher sulfating activity toward 4-nitrophenol adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + bisphenol A Drosophila melanogaster isoform ST3 also shows sulfating activity toward bisphenol A adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + dopamine Drosophila melanogaster isoform ST1 recognizes dopamine as a substrate adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + vanillin Drosophila melanogaster vanillin is the best substrate for isoforms ST1 and ST3 adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 additional information Drosophila melanogaster isoform ST4 shows low but not negligible activity toward 20-hydroxyecdysone ?
-
 ?
2.8.2.1 additional information Drosophila melanogaster no activity toward tyramine and serotonin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 glutathione Sepharose column chromatography and glutathione Sepharose gel filtration Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 3'-phosphoadenylyl sulfate + 1-naphthol isoform ST4 shows a preference toward 1-naphtol Drosophila melanogaster adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + 4-nitrophenol isoform ST3 shows higher sulfating activity toward 4-nitrophenol Drosophila melanogaster adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + bisphenol A isoform ST3 also shows sulfating activity toward bisphenol A Drosophila melanogaster adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + dopamine isoform ST1 recognizes dopamine as a substrate Drosophila melanogaster adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 3'-phosphoadenylyl sulfate + vanillin vanillin is the best substrate for isoforms ST1 and ST3 Drosophila melanogaster adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.1 additional information isoform ST4 shows low but not negligible activity toward 20-hydroxyecdysone Drosophila melanogaster ?
-
 ?
2.8.2.1 additional information no activity toward tyramine and serotonin Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.1 cytosolic sulfotransferase
-
 Drosophila melanogaster
2.8.2.1 ST1 isoform Drosophila melanogaster
2.8.2.1 ST2 isoform Drosophila melanogaster
2.8.2.1 ST3 isoform Drosophila melanogaster
2.8.2.1 ST4 isoform Drosophila melanogaster
2.8.2.1 SULT
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.1 30
-
 isoform ST4 Drosophila melanogaster
2.8.2.1 35
-
 isoform ST1 Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.2.1 8.5
-
 3'-phosphoadenylyl sulfate isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
2.8.2.1 27
-
 3'-phosphoadenylyl sulfate isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
2.8.2.1 30
-
 4-nitrophenol isoform ST1, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
2.8.2.1 40.7
-
 3'-phosphoadenylyl sulfate isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
2.8.2.1 70
-
 4-nitrophenol isoform ST4, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster
2.8.2.1 330
-
 4-nitrophenol isoform ST3, in 50 mM sodium phosphate, pH 6.8, at 37°C Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.1 6.4 6.8 isoform ST3 Drosophila melanogaster

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.8.2.1 additional information
-
 pH has little effect on the activities of isoforms ST1 and ST4 Drosophila melanogaster