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Literature summary extracted from

  • Palmieri-Thiers, C.; Canaan, S.; Brunini, V.; Lorenzi, V.; Tomi, F.; Desseyn, J.L.; Garscha, U.; Oliw, E.H.; Berti, L.; Maury, J.
    A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening (2009), Biochim. Biophys. Acta, 1791, 339-346.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.B6 expression in Escherichia coli Olea europaea
1.13.11.12 vector pQE30-LOX expressed in Escherichia coli M15 cells Olea europaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.B6 nordihydroguaiaretic acid noncompetitive Olea europaea
1.13.11.B6 propyl gallate competitive Olea europaea
1.13.11.12 nordihydroguaiaretic acid is a noncompetitive inhibitor Olea europaea
1.13.11.12 propyl gallate is a competitive inhibitor Olea europaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.B6 0.175
-
 linoleate 25°C Olea europaea
1.13.11.B6 0.344
-
 linolenate 25°C Olea europaea
1.13.11.12 0.1749
-
 linoleic acid pH 6.0, 25°C Olea europaea
1.13.11.12 0.3437
-
 linolenic acid pH 6.0, 25°C Olea europaea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.B6 cytosol likely confined to the cytosol, because it contains neither consensus targeting nor retention signals for any organelles Olea europaea 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.B6 98000
-
 x * 98000, SDS-PAGE Olea europaea
1.13.11.B6 98400
-
 x * 98400, calculated from sequence Olea europaea
1.13.11.12 98000
-
 x * 98000, SDS-PAGE and immunoblot Olea europaea
1.13.11.12 98400
-
 x * 98400, calculated from sequence Olea europaea

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.B6 Olea europaea B6D1W5 L., Leccino variety
-
1.13.11.12 Olea europaea B6D1W5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.B6
-
 Olea europaea
1.13.11.12 by Ni-affinity chromatography and gel filtration, 26fold Olea europaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.B6 fruit detected in all olive developmental and ripening stages from green small fruits to black or senescent fruits. Mainly expressed at late developmental stages Olea europaea
-
1.13.11.12 fruit is mainly expressed at late developmental stages, two Lox genes expressed in black olives. Increase of the LOX activity at the end of the green stage and the turning stage of the olives, and the maximum is reached at the black stage Olea europaea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.B6 23.4
-
-
 Olea europaea
1.13.11.12 23.4
-
 26fold purified enzyme, with linoleic acid as substrate Olea europaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.B6 linoleate + O2 the catalytic efficiency of the recombinant olive LOX was significantly higher for linoleic acid hydroperoxidation than for linolenic acid hydroperoxidation Olea europaea (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E,13R)-13-hydroperoxy-9,11-octadecadienoate dual positional specificity: the enzyme forms (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and (9Z,11E,13R)-13-hydroperoxy-9,11-octadecadienoate in a 2:1 ratio, the products are predominantly in 9S and 13R configuration ?
1.13.11.B6 linolenate + O2 the catalytic efficiency of the recombinant olive LOX is significantly higher for linoleic acid hydroperoxidation than for linolenic acid hydroperoxidation Olea europaea ?
-
 ?
1.13.11.12 linoleic acid + O2
-
 Olea europaea (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid the recombinant enzyme shows a dual positional specificity, as it forms both 9- and 13-hydroperoxy octadecadienoic acid in a ratio 2:1 ?
1.13.11.12 linolenic acid + O2
-
 Olea europaea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.B6 ? x * 98000, SDS-PAGE Olea europaea
1.13.11.B6 ? x * 98400, calculated from sequence Olea europaea
1.13.11.12 ? x * 98000, SDS-PAGE and immunoblot Olea europaea
1.13.11.12 ? x * 98400, calculated from sequence Olea europaea

Synonyms

EC Number Synonyms Comment Organism
1.13.11.B6 9/13-LOX
-
 Olea europaea
1.13.11.12 9/13-LOX
-
 Olea europaea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.B6 47.8
-
 linolenate 25°C Olea europaea
1.13.11.B6 48.6
-
 linoleate 25°C Olea europaea
1.13.11.12 47.78
-
 linolenic acid pH 6.0, 25°C Olea europaea
1.13.11.12 48.16
-
 linoleic acid pH 6.0, 25°C Olea europaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.B6 6
-
-
 Olea europaea
1.13.11.12 6
-
-
 Olea europaea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.B6 0.00188
-
 propyl gallate 25°C Olea europaea
1.13.11.B6 0.00409
-
 nordihydroguaiaretic acid 25°C Olea europaea
1.13.11.12 0.00188
-
 propyl gallate pH 6.0, 25°C Olea europaea
1.13.11.12 0.00409
-
 nordihydroguaiaretic acid pH 6.0, 25°C Olea europaea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.B6 Olea europaea calculated from sequence
-
 5.95
1.13.11.12 Olea europaea calculated from sequence
-
 5.95

General Information

EC Number General Information Comment Organism
1.13.11.B6 physiological function the 9/13-LOX is associated with the ripening and senescence processes. Due to its dual positional specificity and its expression pattern, its contribution to the elaboration of the olive oil aroma might be considered Olea europaea
1.13.11.12 physiological function 9/13-LOX is associated with the ripening and senescence processes Olea europaea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.B6 139
-
 linolenate 25°C Olea europaea
1.13.11.B6 278
-
 linoleate 25°C Olea europaea
1.13.11.12 140
-
 linolenic acid
-
 Olea europaea
1.13.11.12 280
-
 linoleic acid
-
 Olea europaea