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Literature summary extracted from

  • Ghanem, M.; Li, L.; Wing, C.; Schramm, V.L.
    Altered thermodynamics from remote mutations altering human toward bovine purine nucleoside phosphorylase (2008), Biochemistry, 47, 2559-2564.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.1 L22E/H104R mutation of human toward bovine purine nucleoside phosphorylase. Mutant is similar to human purine nucleoside phosphorylase in steady-state kinetic properties. Inhibitor DADMe-immucillin-G is an excellent mimic of the transition states for both human purine nucleoside phosphorylase and the mutant with a preference for the mutant. Thermodynamic parameters establish the mutant to be catalytically more efficient than the parent enzyme and reveal differences in the entropic component of catalysis Homo sapiens
2.4.2.1 L22E/H104R mutation of human toward bovine purine nucleoside phosphorylase. Mutant is similar to human purine nucleoside phosphorylase in steady-state kinetic properties. Inhibitor DADMe-immucillin-G is an excellent mimic of the transition states for both human purine nucleoside phosphorylase and the mutant with a preference for the mutant. Thermodynamic parameters establish the mutant to be catalytically more efficient than the parent enzyme and reveal differences in the entropic component of catalysis Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 DADMe-immucillin-G
-
 Homo sapiens
2.4.2.1 DADMe-immucillin-H
-
 Homo sapiens
2.4.2.1 immucillin-G
-
 Homo sapiens
2.4.2.1 immucillin-H
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.016
-
 Inosine pH 7.4, 25°C Bos taurus
2.4.2.1 0.027
-
 Inosine mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
2.4.2.1 0.03
-
 Inosine wild-tpe, pH 7.4, 25°C Homo sapiens
2.4.2.1 0.042
-
 guanosine wild-tpe, pH 7.4, 25°C Homo sapiens
2.4.2.1 0.047
-
 guanosine mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
2.4.2.1 0.049
-
 guanosine pH 7.4, 25°C Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Bos taurus
-
-
-
2.4.2.1 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 guanosine + phosphate
-
 Homo sapiens guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Bos taurus guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Homo sapiens hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Bos taurus hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 20
-
 Inosine pH 7.4, 25°C Bos taurus
2.4.2.1 27
-
 guanosine pH 7.4, 25°C Bos taurus
2.4.2.1 30
-
 guanosine mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
2.4.2.1 31
-
 guanosine wild-tpe, pH 7.4, 25°C Homo sapiens
2.4.2.1 31.5
-
 Inosine mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
2.4.2.1 40
-
 Inosine wild-tpe, pH 7.4, 25°C Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.1 0.000000034
-
 DADMe-immucillin-G wild-tpe, pH 7.4, 25°C Homo sapiens
2.4.2.1 0.000000121
-
 DADMe-immucillin-G mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
2.4.2.1 0.000000133
-
 immucillin-G wild-tpe, pH 7.4, 25°C Homo sapiens
2.4.2.1 0.000000175
-
 immucillin-G mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
2.4.2.1 0.00000024
-
 DADMe-immucillin-H mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
2.4.2.1 0.00000035
-
 DADMe-immucillin-H wild-tpe, pH 7.4, 25°C Homo sapiens
2.4.2.1 0.00000067
-
 immucillin-H mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
2.4.2.1 0.000001
-
 immucillin-H wild-tpe, pH 7.4, 25°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.1 550
-
 guanosine pH 7.4, 25°C Bos taurus
2.4.2.1 630
-
 guanosine mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
2.4.2.1 730
-
 guanosine wild-tpe, pH 7.4, 25°C Homo sapiens
2.4.2.1 1200
-
 Inosine mutant L22E/H104R, pH 7.4, 25°C Homo sapiens
2.4.2.1 1250
-
 Inosine pH 7.4, 25°C Bos taurus
2.4.2.1 1300
-
 Inosine wild-tpe, pH 7.4, 25°C Homo sapiens