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Literature summary extracted from

  • Sasaki, Y.; Isobe, K.; Kataoka, M.; Ogawa, J.; Iwasaki, A.; Hasegawa, J.; Shimizu, S.
    Purification and characterization of a new aldehyde oxidase from Pseudomonas sp. AIU 362 (2008), J. Biosci. Bioeng., 106, 297-302.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.3.1 Sodium azide 1 mM, 128% of initial activity Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.1 hydrazine 1 mM, 28% residual activity Pseudomonas sp.
1.2.3.1 hydroxyamine 1 mM, 52% residual activity Pseudomonas sp.
1.2.3.1 MgCl2 1 mM, 62% residual activity Pseudomonas sp.
1.2.3.1 phenylhydrazine 1 mM, 12% residual activity Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.1 0.00014
-
 hexylaldehyde 30°C, pH 7.0 Pseudomonas sp.
1.2.3.1 0.0005
-
 Butyraldehyde 30°C, pH 7.0 Pseudomonas sp.
1.2.3.1 0.00057
-
 benzaldehyde 30°C, pH 7.0 Pseudomonas sp.
1.2.3.1 0.0008
-
 p-Methoxybenzaldehyde 30°C, pH 7.0 Pseudomonas sp.
1.2.3.1 0.0011
-
 Valeraldehyde 30°C, pH 7.0 Pseudomonas sp.
1.2.3.1 0.0025
-
 acetaldehyde 30°C, pH 7.0 Pseudomonas sp.
1.2.3.1 0.0026
-
 p-Hydroxybenzaldehyde 30°C, pH 7.0 Pseudomonas sp.
1.2.3.1 0.0028
-
 propionaldehyde 30°C, pH 7.0 Pseudomonas sp.
1.2.3.1 0.0097
-
 Glyoxal 30°C, pH 7.0 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.3.1 CuCl2 1 mM, 123% of initial activity Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.3.1 27000
-
 4 * 27000, SDS-PAGE Pseudomonas sp.
1.2.3.1 95000
-
 PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.1 Pseudomonas sp.
-
-
-
1.2.3.1 Pseudomonas sp. AIU 362
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.1
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.1 acetaldehyde + H2O + O2
-
 Pseudomonas sp. acetic acid + H2O2
-
 ?
1.2.3.1 acetaldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 acetic acid + H2O2
-
 ?
1.2.3.1 benzaldehyde + H2O + O2
-
 Pseudomonas sp. benzoate + H2O2
-
 ?
1.2.3.1 benzaldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 benzoate + H2O2
-
 ?
1.2.3.1 butyraldehyde + H2O + O2
-
 Pseudomonas sp. butanoate + H2O2
-
 ?
1.2.3.1 glyoxal + H2O + O2
-
 Pseudomonas sp. glyoxylate + H2O2
-
 ?
1.2.3.1 hexylaldehyde + H2O + O2
-
 Pseudomonas sp. hexanoate + H2O2
-
 ?
1.2.3.1 hexylaldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 hexanoate + H2O2
-
 ?
1.2.3.1 p-hydroxybenzaldehyde + H2O + O2
-
 Pseudomonas sp. p-hydroxybenzoate + H2O2
-
 ?
1.2.3.1 p-methoxybenzaldehyde + H2O + O2
-
 Pseudomonas sp. p-methoxybenzoate + H2O2
-
 ?
1.2.3.1 propionaldehyde + H2O + O2
-
 Pseudomonas sp. propionic acid + H2O2
-
 ?
1.2.3.1 propionaldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 propionic acid + H2O2
-
 ?
1.2.3.1 valeraldehyde + H2O + O2
-
 Pseudomonas sp. valeric acid + H2O2
-
 ?
1.2.3.1 valeraldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 valeric acid + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.3.1 tetramer 4 * 27000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.3.1 40 45
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.3.1 30 55 enzyme retains at least 80% of initial activity for 30 min Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.1 6
-
 substrate glyoxal Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.3.1 7 8.5 30°C, retains more than 80% of initial activity for at least 2 h Pseudomonas sp.