EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | additional information | expression of AdhA is enhanced upon the exposure of cells to different environmental stresses, e.g., hyperosmotic stress (0.5 M sorbitol), salt stress (0.5 M NaCl) or increase in the ambient temperature (45°C). Neither high-light treatment nor oxidative stress (1 mM H2O2) has an influence on the transcription of the adhA gene. Benzyl alcohol also induces adhA gene expression. The induction of the expression of the adhA gene is dependent on the Hik34-Rre1 two-component system. The response regulator Rre1 binds to the promoter region of the adhA gene | Synechocystis sp. | |
1.1.1.2 | additional information | the induction of the expression of the adhA gene is dependent on the Hik34-Rre1 two-component system in response to stress conditions | Synechocystis sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.2 | expressed in Escherichia coli BL21(DE3) cells | Synechocystis sp. |
1.1.1.2 | into the pET-28a(+) vector, plasmid pETAdhA expressed in Escherichia coli BL21(DE3) | Synechocystis sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.2 | additional information | adhA disruption mutant shows no differences in responses to heat shock, salt, or hyperosmotic stress as compared to the wild-type. The adhA mutant strain is unable to grow heterotrophically (added glucose) in darkness, contrary to the wild-type strain | Synechocystis sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.2 | 0.017 | - |
cinnamaldehyde | - |
Synechocystis sp. | |
1.1.1.2 | 0.017 | - |
cinnamaldehyde | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 0.025 | - |
NADPH | - |
Synechocystis sp. | |
1.1.1.2 | 0.025 | - |
NADPH | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 0.05 | - |
NADP+ | - |
Synechocystis sp. | |
1.1.1.2 | 0.05 | - |
NADP+ | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 0.075 | - |
pentanal | - |
Synechocystis sp. | |
1.1.1.2 | 0.075 | - |
pentanal | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 0.11 | - |
Butanal | - |
Synechocystis sp. | |
1.1.1.2 | 0.11 | - |
Butanal | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 0.27 | - |
cinnamyl alcohol | - |
Synechocystis sp. | |
1.1.1.2 | 0.27 | - |
cinnamyl alcohol | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 0.51 | - |
acetaldehyde | - |
Synechocystis sp. | |
1.1.1.2 | 0.51 | - |
acetaldehyde | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 1.31 | - |
NADH | - |
Synechocystis sp. | |
1.1.1.2 | 1.31 | - |
NADH | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 1.54 | - |
Pentanol | - |
Synechocystis sp. | |
1.1.1.2 | 1.54 | - |
Pentanol | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 3.28 | - |
butanol | - |
Synechocystis sp. | |
1.1.1.2 | 3.28 | - |
butanol | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 9.7 | - |
NAD+ | - |
Synechocystis sp. | |
1.1.1.2 | 9.7 | - |
NAD+ | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 18.8 | - |
ethanol | - |
Synechocystis sp. | |
1.1.1.2 | 18.8 | - |
ethanol | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.195 | 0.017 | - |
cinnamaldehyde | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.195 | 0.27 | - |
cinnamyl alcohol | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.2 | cytosol | - |
Synechocystis sp. | 5829 | - |
1.1.1.195 | cytosol | - |
Synechocystis sp. | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | Co2+ | - |
Synechocystis sp. | |
1.1.1.2 | Zn2+ | - |
Synechocystis sp. | |
1.1.1.2 | Zn2+ | contains zinc | Synechocystis sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 36700 | - |
3 * 36700, SDS-PAGE | Synechocystis sp. |
1.1.1.2 | 36700 | - |
4 * 36700, estimated by SDS-PAGE | Synechocystis sp. |
1.1.1.2 | 140000 | - |
gel filtration | Synechocystis sp. |
1.1.1.2 | 140000 | - |
SDS-PAGE | Synechocystis sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.2 | Synechocystis sp. | - |
- |
- |
1.1.1.2 | Synechocystis sp. | - |
strain PCC 6803 | - |
1.1.1.195 | Synechocystis sp. | - |
strain PCC 6803 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.2 | by anion-exchange chromatography followed by affinity chromatography on Ni-NTA | Synechocystis sp. |
1.1.1.2 | DEAE-cellulose resin column chromatography, Ni-NTA column chromatography, and Superose 6 gel filtration | Synechocystis sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.2 | 1-propanol + NADP+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 21% relative activity with 1 mM or 84% relative activity with 10 mM substrate | Synechocystis sp. | propanal + NADPH + H+ | - |
r | |
1.1.1.2 | 2-methylbutanol + NADP+ | 24% activity with 1 mM 2-methylbutanol, 22% activity with 10 mM 2-methylbutanol | Synechocystis sp. | 2-methylbutyraldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | 2-methylbutanol + NADP+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 24% relative activity with 1 mM or 22% relative activity with 10 mM substrate | Synechocystis sp. | ? + NADPH + H+ | - |
? | |
1.1.1.2 | acetaldehyde + NADPH + H+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 1% relative activity with 0.1 mM or 23% relative activity with 1 mM substrate | Synechocystis sp. | ethanol + NADP+ | - |
r | |
1.1.1.2 | benzaldehyde + NADPH + H+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 30% relative activity with 0.1 mM or 14% relative activity with 1 mM substrate | Synechocystis sp. | benzyl alcohol + NADP+ | - |
r | |
1.1.1.2 | benzyl alcohol + NADP+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 42% relative activity with 1 mM or 79% relative activity with 10 mM substrate | Synechocystis sp. | benzaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | benzyl alcohol + NADP+ | 42% activity with 1 mM benzyl alcohol, 79% activity with 10 mM benzyl alcohol | Synechocystis sp. | benzaldehyde + NADPH + H+ | 30% activity with 0.1 mM benzaldehyde, 14% activity with 1 mM benzaldehyde | r | |
1.1.1.2 | butanal + NADPH + H+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 73% relative activity with 0.1 mM or 120% relative activity with 1 mM substrate | Synechocystis sp. | butanol + NADP+ | - |
r | |
1.1.1.2 | butanol + NADP+ | 23% activity with 1 mM butanol, 70% activity with 10 mM butanol | Synechocystis sp. | butanal + NADPH + H+ | 73% activity with 0.1 mM butanal, 120% activity with 1 mM butanal | r | |
1.1.1.2 | butanol + NADP+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 23% relative activity with 1 mM or 70% relative activity with 10 mM substrate | Synechocystis sp. | butanal + NADPH + H+ | - |
r | |
1.1.1.2 | cinnamaldehyde + NADPH + H+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 61% relative activity with 0.1 mM or 7% relative activity with 1 mM substrate | Synechocystis sp. | cinnamyl alcohol + NADP+ | - |
r | |
1.1.1.2 | cinnamyl alcohol + NADP+ | 100% activity with 1 mM cinnamyl alcohol, 116% activity with 10 mM hexanol | Synechocystis sp. | cinnamaldehyde + NADPH + H+ | 61% activity with 0.1 mM cinnamaldehyde, 7% activity with 1 mM cinnamaldehyde | r | |
1.1.1.2 | cinnamyl alcohol + NADP+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 100% relative activity with 1 mM or 116% relative activity with 10 mM substrate | Synechocystis sp. | cinnamaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | decanal + NADPH + H+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 21% relative activity with 0.1 mM or 22% relative activity with 1 mM substrate | Synechocystis sp. | decanol + NADP+ | - |
r | |
1.1.1.2 | decanol + NADP+ | 3% activity with 1 mM decanol, 6% activity with 10 mM decanol | Synechocystis sp. | decanal + NADPH + H+ | 21% activity with 0.1 mM decanal, 22% activity with 1 mM decanal | r | |
1.1.1.2 | decanol + NADP+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 3% relative activity with 1 mM or 6% relative activity with 10 mM substrate | Synechocystis sp. | decanal + NADPH + H+ | - |
r | |
1.1.1.2 | ethanol + NAD+ | - |
Synechocystis sp. | acetaldehyde + NADH + H+ | - |
r | |
1.1.1.2 | ethanol + NADP+ | 19% activity with 1 mM ethanol, 91% activity with 10 mM ethanol | Synechocystis sp. | acetaldehyde + NADPH + H+ | 1% activity with 0.1 mM acetaldehyde, 23% activity with 1 mM acetaldehyde | r | |
1.1.1.2 | ethanol + NADP+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 19% relative activity with 1 mM or 91% relative activity with 10 mM substrate | Synechocystis sp. | acetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | hexanal + NADPH + H+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 53% relative activity with 0.1 mM or 100% relative activity with 1 mM substrate | Synechocystis sp. | hexanol + NADP+ | - |
r | |
1.1.1.2 | hexanol + NADP+ | 27% activity with 1 mM hexanol, 40% activity with 10 mM hexanol | Synechocystis sp. | hexanal + NADPH + H+ | 53% activity with 0.1 mM hexanal, 100% activity with 1 mM hexanal | r | |
1.1.1.2 | hexanol + NADP+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 27% relative activity with 1 mM or 40% relative activity with 10 mM substrate | Synechocystis sp. | hexanal + NADPH + H+ | - |
r | |
1.1.1.2 | additional information | no activity with acetone, 3-pentanone, D-glucose, and 3-pentanol | Synechocystis sp. | ? | - |
? | |
1.1.1.2 | octanal + NADPH + H+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 42% relative activity with 0.1 mM or 80% relative activity with 1 mM substrate | Synechocystis sp. | octanol + NADP+ | - |
r | |
1.1.1.2 | octanol + NADP+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 8% relative activity with 1 mM or 17% relative activity with 10 mM substrate | Synechocystis sp. | octanal + NADPH + H+ | - |
r | |
1.1.1.2 | octanol + NADP+ | 8% activity with 1 mM octanol, 17% activity with 10 mM octanol | Synechocystis sp. | octanal + NADPH + H+ | 42% activity with 0.1 mM octanal, 80% activity with 1 mM octanal | r | |
1.1.1.2 | pentanal + NADPH + H+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 100% relative activity with 0.1 mM or 107% relative activity with 1 mM substrate | Synechocystis sp. | pentanol + NADP+ | - |
r | |
1.1.1.2 | pentanol + NADP+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 31% relative activity with 1 mM or 52% relative activity with 10 mM substrate | Synechocystis sp. | pentanal + NADPH + H+ | - |
r | |
1.1.1.2 | pentanol + NADP+ | 31% activity with 1 mM pentanol, 52% activity with 10 mM pentanol | Synechocystis sp. | pentanal + NADPH + H+ | 100% activity with 0.1 mM pentanal, 107% activity with 1 mM pentanal | r | |
1.1.1.2 | phenylethanol + NADP+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.5 mM NADP+, 15% relative activity with 1 mM or 24% relative activity with 10 mM substrate | Synechocystis sp. | ? + NADPH + H+ | - |
? | |
1.1.1.2 | phenylethanol + NADP+ | 15% activity with 1 mM phenylethanol, 24% activity with 10 mM phenylethanol | Synechocystis sp. | phenylacetaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | propanal + NADPH + H+ | 30 mM Tris-HCl (pH 8.0) in the presence of 0.2 mM NADPH, 26% relative activity with 0.1 mM or 87% relative activity with 1 mM substrate | Synechocystis sp. | 1-propanol + NADP+ | - |
r | |
1.1.1.2 | propanol + NADP+ | 21% activity with 1 mM propanol, 84% activity with 10 mM propanol | Synechocystis sp. | propanal + NADPH + H+ | 26% activity with 0.1 mM propanal, 87% activity with 1 mM propanal | r | |
1.1.1.195 | cinnamyl alcohol + NADP+ | 100% activity with 1 mM cinnamyl alcohol, 116% activity with 10 mM cinnamyl alcohol, reaction catalyzed by unspecific alcohol dehydrogenase (EC 1.1.1.1) | Synechocystis sp. | cinnamaldehyde + NADPH + H+ | 61% activity with 0.1 mM cinnamaldehyde, 7% activity with 1 mM cinnamaldehyde | r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.2 | homotetramer | 4 * 36700, estimated by SDS-PAGE | Synechocystis sp. |
1.1.1.2 | tetramer | 3 * 36700, SDS-PAGE | Synechocystis sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.2 | AdhA | - |
Synechocystis sp. |
1.1.1.195 | AdhA | - |
Synechocystis sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.2 | 13.15 | - |
Pentanol | - |
Synechocystis sp. | |
1.1.1.2 | 13.15 | - |
Pentanol | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 17.05 | - |
butanol | - |
Synechocystis sp. | |
1.1.1.2 | 17.05 | - |
butanol | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 27.35 | - |
ethanol | - |
Synechocystis sp. | |
1.1.1.2 | 27.35 | - |
ethanol | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 60.78 | - |
cinnamyl alcohol | - |
Synechocystis sp. | |
1.1.1.2 | 60.78 | - |
cinnamyl alcohol | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 107.9 | - |
cinnamaldehyde | - |
Synechocystis sp. | |
1.1.1.2 | 107.9 | - |
cinnamaldehyde | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 156 | - |
Butanal | - |
Synechocystis sp. | |
1.1.1.2 | 156.6 | - |
Butanal | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 157.6 | - |
acetaldehyde | - |
Synechocystis sp. | |
1.1.1.2 | 157.6 | - |
acetaldehyde | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.2 | 159 | - |
pentanal | - |
Synechocystis sp. | |
1.1.1.2 | 159.1 | - |
pentanal | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.195 | 60.78 | - |
cinnamyl alcohol | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. | |
1.1.1.195 | 107.9 | - |
cinnamaldehyde | in 30 mM Tris-HCl (pH 8.0) | Synechocystis sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.2 | NAD+ | - |
Synechocystis sp. | |
1.1.1.2 | NADH | - |
Synechocystis sp. | |
1.1.1.2 | NADH | the enzyme can use both NADPH and NADH as electron donors, although NADPH is preferred to NADH | Synechocystis sp. | |
1.1.1.2 | NADP+ | affinity for NADP+ is higher than that for NAD+ as an alcohol oxidant | Synechocystis sp. | |
1.1.1.2 | NADPH | NADPH is preferred to NADH as the reductant for aldehydes | Synechocystis sp. | |
1.1.1.2 | NADPH | the enzyme can use both NADPH and NADH as electron donors, although NADPH is preferred to NADH | Synechocystis sp. |