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Literature summary extracted from

  • Ota, H.; Sakasegawa, S.; Yasuda, Y.; Imamura, S.; Tamura, T.
    A novel nucleoside kinase from Burkholderia thailandensis: a member of the phosphofructokinase B-type family of enzymes (2008), FEBS J., 275, 5865-5872.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.B20 functionally overexpressed in Rhodococcus erythropolis Burkholderia thailandensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.B20 adenosine concentrations above 0.1 mM lead to reduction in enzyme activity Burkholderia thailandensis
2.7.1.B20 Inosine concentrations above 0.1 mM lead to reduction in enzyme activity Burkholderia thailandensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.B20 0.02
-
 adenosine pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 0.15
-
 Inosine pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 0.46
-
 guanosine pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 0.6 3 ATP pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 0.79
-
 ribavirin pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 0.87
-
 mizoribine pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 1.1
-
 2'-deoxyadenosine pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 1.7
-
 uridine pH 7.0, 37°C Burkholderia thailandensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.B20 Co2+ enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+ Burkholderia thailandensis
2.7.1.B20 Mg2+ enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+. Maximum activity is attained when the ATP/Mg2+ concentration ratio is 0.5 Burkholderia thailandensis
2.7.1.B20 Ni2+ enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+ Burkholderia thailandensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.B20 34000
-
 2 * 34000, SDS-PAGE Burkholderia thailandensis
2.7.1.B20 64000
-
 gel filtration Burkholderia thailandensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.B20 Burkholderia thailandensis Q2SZE4
-
-
2.7.1.B20 Burkholderia thailandensis DSM 13276 Q2SZE4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.B20
-
 Burkholderia thailandensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.B20 ATP + 2'-deoxyadenosine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.B20 ATP + 2'-deoxyadenosine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis DSM 13276 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.B20 ATP + adenosine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis ADP + adenosine 5'-phosphate
-
 ?
2.7.1.B20 ATP + adenosine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis DSM 13276 ADP + adenosine 5'-phosphate
-
 ?
2.7.1.B20 ATP + guanosine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis ADP + guanosine 5'-phosphate
-
 ?
2.7.1.B20 ATP + inosine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis ADP + inosine 5'-phosphate
-
 ?
2.7.1.B20 ATP + mizoribine i.e. 5-hydroxy-1-beta-D-ribofuranosyl-1H-imidazole-4-carboxamide. Phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis ADP + mizoribine 5'-phosphate
-
 ?
2.7.1.B20 ATP + ribavirin phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis ADP + ribavirin 5'-phosphate
-
 ?
2.7.1.B20 ATP + ribavirin phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis DSM 13276 ADP + ribavirin 5'-phosphate
-
 ?
2.7.1.B20 ATP + uridine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis ADP + uridine 5'-phosphate
-
 ?
2.7.1.B20 ATP + uridine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis DSM 13276 ADP + uridine 5'-phosphate
-
 ?
2.7.1.B20 CTP + inosine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis CDP + inosine 5'-phosphate
-
 ?
2.7.1.B20 GTP + inosine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis GDP + inosine 5'-phosphate
-
 ?
2.7.1.B20 ITP + inosine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis IDP + inosine 5'-phosphate
-
 ?
2.7.1.B20 additional information in the presence of D-ribose, L-ribose, fructose, glucose, 2-deoxy-glucose, xanthosine, galactose, xylose, N-acetyl glucosamine, fructose-6-phosphate, mannose-6-phosphate and ribose-5-phosphate, the activity is below the detection limit. No activity with cytidine or thamidine Burkholderia thailandensis ?
-
 ?
2.7.1.B20 additional information in the presence of D-ribose, L-ribose, fructose, glucose, 2-deoxy-glucose, xanthosine, galactose, xylose, N-acetyl glucosamine, fructose-6-phosphate, mannose-6-phosphate and ribose-5-phosphate, the activity is below the detection limit. No activity with cytidine or thamidine Burkholderia thailandensis DSM 13276 ?
-
 ?
2.7.1.B20 TTP + inosine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis TDP + inosine 5'-phosphate
-
 ?
2.7.1.B20 UTP + inosine phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) Burkholderia thailandensis UDP + inosine 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.B20 homodimer 2 * 34000, SDS-PAGE Burkholderia thailandensis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.B20 BTH_I1158 locus name Burkholderia thailandensis
2.7.1.B20 nucleoside kinase
-
 Burkholderia thailandensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.B20 35
-
 pH 7.0, 20 min, stable Burkholderia thailandensis
2.7.1.B20 60
-
 pH 7.0, 20 min, 60% loss of activity Burkholderia thailandensis
2.7.1.B20 70
-
 pH 7.0, 20 min, complete inactivation Burkholderia thailandensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.B20 6 10 37°C, 3 h, stable Burkholderia thailandensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.B20 0.04
-
 mizoribine pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 0.08
-
 ribavirin pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 0.24
-
 guanosine pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 0.28
-
 uridine pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 0.84
-
 2'-deoxyadenosine pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 6.05
-
 adenosine pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 16
-
 ATP pH 7.0, 37°C Burkholderia thailandensis
2.7.1.B20 80
-
 Inosine pH 7.0, 37°C Burkholderia thailandensis
2.7.1.143 0.04
-
 mizoribine
-
 Burkholderia thailandensis
2.7.1.143 0.08
-
 ribavirin
-
 Burkholderia thailandensis
2.7.1.143 0.24
-
 guanosine
-
 Burkholderia thailandensis
2.7.1.143 0.28
-
 uridine
-
 Burkholderia thailandensis
2.7.1.143 0.84
-
 2-deoxy-adenosine
-
 Burkholderia thailandensis
2.7.1.143 6.05
-
 adenosine
-
 Burkholderia thailandensis
2.7.1.143 16
-
 ATP
-
 Burkholderia thailandensis
2.7.1.143 80
-
 Inosine inosine is the most effective phosphoryl acceptor, with the highest kcat /Km value being achieved when ATP served as the phosphoryl donor Burkholderia thailandensis