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Literature summary extracted from

  • Kato, T.; Miyanaga, A.; Kanaya, S.; Morikawa, M.
    Gene cloning and characterization of an aldehyde dehydrogenase from long-chain alkane-degrading Geobacillus thermoleovorans B23 (2009), Extremophiles, 14, 33-39.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.48 alkane transcriptional induction of Bt-aldh gene by alkane, Bt-aldh dramatically increases after 10 day cultivation, exactly when the alkane degradation starts Geobacillus thermoleovorans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3
-
 Geobacillus thermoleovorans
1.2.1.48 expression and overproduction in Escherichia coli Geobacillus thermoleovorans
1.2.1.48 expression in Escherichia coli Geobacillus thermoleovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 Mg2+
-
 Geobacillus thermoleovorans
1.2.1.3 Zn2+
-
 Geobacillus thermoleovorans
1.2.1.48 EDTA 2 mM, 63% residual activity; 63% relative activity Geobacillus thermoleovorans
1.2.1.48 Mg2+ 14% relative activity with 2 mM MgCl2 compared to the activity without metal ions; 2 mM, 14% residual activity Geobacillus thermoleovorans
1.2.1.48 Zn2+ 2 mM, 0.1% residual activity; relative activity is above 1% with 2 mM ZnCl2 compared to the activity without metal ions Geobacillus thermoleovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.3 Ba2+ the activity is elevated to 2-3fold in the presence of 2 mM Ba2+ Geobacillus thermoleovorans
1.2.1.3 Ca2+ the activity is elevated to 2-3fold in the presence of 2 mM Ca2+ Geobacillus thermoleovorans
1.2.1.3 Sr2+ the activity is elevated to 2-3fold in the presence of 2 mM Sr2+ Geobacillus thermoleovorans
1.2.1.48 Ba2+ activity is elevated 2fold in the presence of 2 mM Geobacillus thermoleovorans
1.2.1.48 Ba2+ 2 mM, 2-3fold stimulation Geobacillus thermoleovorans
1.2.1.48 Ca2+ activity is elevated 2fold in the presence of 2 mM Geobacillus thermoleovorans
1.2.1.48 Ca2+ 2 mM, 2-3fold stimulation Geobacillus thermoleovorans
1.2.1.48 Sr2+ activity is elevated 3fold in the presence of 2 mM Geobacillus thermoleovorans
1.2.1.48 Sr2+ 2 mM, 2-3fold stimulation Geobacillus thermoleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 53886
-
 8 * 53886, calculated from amino acid sequence Geobacillus thermoleovorans
1.2.1.48 53886
-
 8 * 53886, calculated, 8 * 55000, SDS-PAGE Geobacillus thermoleovorans
1.2.1.48 53890
-
 monomer is calculated from the 497 amino acid residues Geobacillus thermoleovorans
1.2.1.48 55000
-
 8 * 55000, determined by SDS-PAGE Geobacillus thermoleovorans
1.2.1.48 55000
-
 8 * 53886, calculated, 8 * 55000, SDS-PAGE Geobacillus thermoleovorans
1.2.1.48 410000
-
 gel filtration Geobacillus thermoleovorans
1.2.1.48 410000
-
 estimated by gel filtration Geobacillus thermoleovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.48 acetaldehyde + NAD+ + H2O Geobacillus thermoleovorans
-
 acetate + NADH + H+
-
 ?
1.2.1.48 acetaldehyde + NAD+ + H2O Geobacillus thermoleovorans B23
-
 acetate + NADH + H+
-
 ?
1.2.1.48 benzaldehyde + NAD+ + H2O Geobacillus thermoleovorans
-
 benzoate + NADH + H+
-
 ?
1.2.1.48 benzaldehyde + NAD+ + H2O Geobacillus thermoleovorans B23
-
 benzoate + NADH + H+
-
 ?
1.2.1.48 betaine aldehyde + NAD+ + H2O Geobacillus thermoleovorans
-
 ?
-
 ?
1.2.1.48 glyceraldehyde + NAD+ + H2O Geobacillus thermoleovorans
-
 ?
-
 ?
1.2.1.48 hexanal + NAD+ + H2O Geobacillus thermoleovorans
-
 ?
-
 ?
1.2.1.48 additional information Geobacillus thermoleovorans enzyme has high activity against aliphatic long-chain aldehydes such as tetradecanal, Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes ?
-
 ?
1.2.1.48 additional information Geobacillus thermoleovorans B23 enzyme has high activity against aliphatic long-chain aldehydes such as tetradecanal, Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes ?
-
 ?
1.2.1.48 octanal + NAD+ + H2O Geobacillus thermoleovorans
-
 octanoate + NADH + H+
-
 ?
1.2.1.48 octanal + NAD+ + H2O Geobacillus thermoleovorans B23
-
 octanoate + NADH + H+
-
 ?
1.2.1.48 p-cumic aldehyde + NAD+ + H2O Geobacillus thermoleovorans
-
 ?
-
 ?
1.2.1.48 retinal + NAD+ + H2O Geobacillus thermoleovorans
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Geobacillus thermoleovorans
-
-
-
1.2.1.3 Geobacillus thermoleovorans B23
-
-
-
1.2.1.48 Geobacillus thermoleovorans Q9FAB1
-
-
1.2.1.48 Geobacillus thermoleovorans B23 Q9FAB1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 ammonium sulfate precipitation, column chromatography, gel filtration Geobacillus thermoleovorans
1.2.1.48 crude enzyme solution is applied to an anionic exchange column and eluted by linearly increasing the NaCl concentration from 0-1.0 M in 50 mM Tris-HCl at pH 7.0 Geobacillus thermoleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.48 additional information
-
 110% relative activity with 2 mM MnCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
1.2.1.48 additional information
-
 120% relative activity with 2 mM CoCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
1.2.1.48 additional information
-
 14% relative activity with 2 mM MgCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
1.2.1.48 additional information
-
 190% relative activity with 2 mM CaCl2 EDTA compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
1.2.1.48 additional information
-
 210% relative activity with 2 mM BaCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
1.2.1.48 additional information
-
 340% relative activity with 2 mM SrCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
1.2.1.48 additional information
-
 63% relative activity with 2 mM EDTA compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
1.2.1.48 additional information
-
 87% relative activity with 2 mM CuCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
1.2.1.48 additional information
-
 97% relative activity with 2 mM NiCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
1.2.1.48 additional information
-
 in the range of substrate aldehydes with carbon numbers C2 to C14, Bt-Aldh is the most active against C14, tetradecanal at 55°C, the activity against tetradecanal is 73times higher than that against octanal Geobacillus thermoleovorans
1.2.1.48 additional information
-
 relative activity is above 1% with 2 mM ZnCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 tetradecanal + NAD+ + H2O high activity Geobacillus thermoleovorans tetradecanoate + NADH
-
 ?
1.2.1.3 tetradecanal + NAD+ + H2O high activity Geobacillus thermoleovorans B23 tetradecanoate + NADH
-
 ?
1.2.1.48 acetaldehyde + NAD+ + H2O
-
 Geobacillus thermoleovorans acetate + NADH + H+
-
 ?
1.2.1.48 acetaldehyde + NAD+ + H2O
-
 Geobacillus thermoleovorans B23 acetate + NADH + H+
-
 ?
1.2.1.48 benzaldehyde + NAD+ + H2O
-
 Geobacillus thermoleovorans benzoate + NADH + H+
-
 ?
1.2.1.48 benzaldehyde + NAD+ + H2O
-
 Geobacillus thermoleovorans B23 benzoate + NADH + H+
-
 ?
1.2.1.48 betaine aldehyde + NAD+ + H2O
-
 Geobacillus thermoleovorans ?
-
 ?
1.2.1.48 dodecanal + NAD+ + H2O 50% of the activity with tetradecanal Geobacillus thermoleovorans dodecanoic acid + NADH + H+
-
 ?
1.2.1.48 glyceraldehyde + NAD+ + H2O
-
 Geobacillus thermoleovorans ?
-
 ?
1.2.1.48 hexanal + NAD+ + H2O
-
 Geobacillus thermoleovorans ?
-
 ?
1.2.1.48 hexanal + NAD+ + H2O 50% of the activity with tetradecanal Geobacillus thermoleovorans hexanoic acid + NADH + H+
-
 ?
1.2.1.48 additional information enzyme has high activity against aliphatic long-chain aldehydes such as tetradecanal, Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes Geobacillus thermoleovorans ?
-
 ?
1.2.1.48 additional information enzyme displays high activity against aliphatic long-chain aldehydes such as tetradecanal Geobacillus thermoleovorans ?
-
 ?
1.2.1.48 additional information enzyme has high activity against aliphatic long-chain aldehydes such as tetradecanal, Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes Geobacillus thermoleovorans B23 ?
-
 ?
1.2.1.48 additional information enzyme displays high activity against aliphatic long-chain aldehydes such as tetradecanal Geobacillus thermoleovorans B23 ?
-
 ?
1.2.1.48 octanal + NAD+ + H2O
-
 Geobacillus thermoleovorans octanoate + NADH + H+
-
 ?
1.2.1.48 octanal + NAD+ + H2O
-
 Geobacillus thermoleovorans B23 octanoate + NADH + H+
-
 ?
1.2.1.48 octanal + NAD+ + H2O 50% of the activity with tetradecanal Geobacillus thermoleovorans octanoic acid + NADH + H+
-
 ?
1.2.1.48 p-cumic aldehyde + NAD+ + H2O
-
 Geobacillus thermoleovorans ?
-
 ?
1.2.1.48 retinal + NAD+ + H2O
-
 Geobacillus thermoleovorans ?
-
 ?
1.2.1.48 tetradecanal + NAD+ + H2O
-
 Geobacillus thermoleovorans tetradecanoic acid + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 homooctamer 8 * 53886, calculated from amino acid sequence Geobacillus thermoleovorans
1.2.1.48 octamer 8 * 55000, determined by SDS-PAGE Geobacillus thermoleovorans
1.2.1.48 octamer 8 * 53886, calculated, 8 * 55000, SDS-PAGE Geobacillus thermoleovorans

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 Bt-Aldh
-
 Geobacillus thermoleovorans
1.2.1.48 Bt-Aldh
-
 Geobacillus thermoleovorans
1.2.1.48 long-chain-aldehyde dehydrogenase
-
 Geobacillus thermoleovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.3 50 55
-
 Geobacillus thermoleovorans
1.2.1.48 50 55
-
 Geobacillus thermoleovorans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.48 50 55 optimum condition for activity Geobacillus thermoleovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.48 55
-
 stable up to Geobacillus thermoleovorans
1.2.1.48 70
-
 activity decreases to 12.7% after a heat treatment at 70°C for 30 min Geobacillus thermoleovorans
1.2.1.48 70
-
 30 min, 12.7% residual activity Geobacillus thermoleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 10
-
-
 Geobacillus thermoleovorans
1.2.1.48 10
-
-
 Geobacillus thermoleovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Geobacillus thermoleovorans
1.2.1.48 NAD+ activity of Bt-Aldh is doubled in the presence of NAD+ than NADP+ Geobacillus thermoleovorans
1.2.1.48 NADH
-
 Geobacillus thermoleovorans
1.2.1.48 NADP+
-
 Geobacillus thermoleovorans
1.2.1.48 NADPH about 50% of the activity with NADH Geobacillus thermoleovorans

Expression

EC Number Organism Comment Expression
1.2.1.3 Geobacillus thermoleovorans transcription level of Bt-aldh is increased upon alkane degradation up
1.2.1.48 Geobacillus thermoleovorans expression level is dramatically increased when alkane degradation is started up