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Literature summary extracted from

  • Sridevi, N.; Srivastava, S.; Khan, B.M.; Prabhune, A.A.
    Characterization of the smallest dimeric bile salt hydrolase from a thermophile Brevibacillus sp (2009), Extremophiles, 13, 363-370.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.24 FeSO4 114.7% residual activity Brevibacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.24 5,5'-dithiobis-2-nitrobenzoic acid 65% residual activity Brevibacillus sp.
3.5.1.24 AgNO3 77.3% residual activity Brevibacillus sp.
3.5.1.24 BaCl2 78.4% residual activity Brevibacillus sp.
3.5.1.24 citraconic anhydride 33.3% residual activity Brevibacillus sp.
3.5.1.24 CoCl2 90.9% residual activity Brevibacillus sp.
3.5.1.24 CuSO4 0% residual activity Brevibacillus sp.
3.5.1.24 EDTA 80.69% residual activity Brevibacillus sp.
3.5.1.24 HgCl2 43.2% residual activity Brevibacillus sp.
3.5.1.24 MgSO4 87.5% residual activity Brevibacillus sp.
3.5.1.24 MnSO4 95.5% residual activity Brevibacillus sp.
3.5.1.24 N-bromosuccinimide 63.6% residual activity Brevibacillus sp.
3.5.1.24 NiSO4 92.0% residual activity Brevibacillus sp.
3.5.1.24 Phenylglyoxal 57.4% residual activity Brevibacillus sp.
3.5.1.24 Trinitrobenzenesulfonic acid 30.9% residual activity Brevibacillus sp.
3.5.1.24 Woodward's reagent K 53.6% residual activity Brevibacillus sp.
3.5.1.24 ZnSO4 76.1% residual activity Brevibacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.24 0.00308
-
 glycodeoxycholic acid apparent value, calculated from a Lineweaver-Burk plot Brevibacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.24 28000
-
 monomer, determined by SDS-PAGE Brevibacillus sp.
3.5.1.24 28200
-
 monomer, determined by MALDI-TOF analysis Brevibacillus sp.
3.5.1.24 56000
-
 native protein, determined by gel filtration Brevibacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.24 Brevibacillus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.24 purified to homogeneity using Q-Sepharose chromatography Brevibacillus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.24 1.03
-
 purification step sonification Brevibacillus sp.
3.5.1.24 12.2
-
 purification step first Q-Sepharose chromatograpgy Brevibacillus sp.
3.5.1.24 13.8
-
 purification step second Q-Sepharose chromatograpgy Brevibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.24 glycochenodeoxycholic acid + H2O
-
 Brevibacillus sp. 3alpha,7alpha-dihydroxy-5beta-cholanate + Gly
-
 ?
3.5.1.24 glycocholic acid + H2O
-
 Brevibacillus sp. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + Gly
-
 ?
3.5.1.24 glycodeoxycholic acid + H2O
-
 Brevibacillus sp. 3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
 ?
3.5.1.24 taurochenodeoxycholic acid + H2O
-
 Brevibacillus sp. chenodeoxycholate + taurine
-
 ?
3.5.1.24 taurocholic acid + H2O
-
 Brevibacillus sp. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
 ?
3.5.1.24 taurodeoxycholic acid + H2O
-
 Brevibacillus sp. 3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.24 homodimer
-
 Brevibacillus sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.24 bile salt hydrolase
-
 Brevibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.24 40
-
 activity assay Brevibacillus sp.
3.5.1.24 60
-
-
 Brevibacillus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.24 632
-
 glycodeoxycholic acid apparent value, calculated from a Lineweaver-Burk plot Brevibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.24 6.5
-
 activity assay Brevibacillus sp.
3.5.1.24 9
-
-
 Brevibacillus sp.