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Literature summary extracted from

  • Pal, S.; Park, D.H.; Plapp, B.V.
    Activity of yeast alcohol dehydrogenases on benzyl alcohols and benzaldehydes: characterization of ADH1 from Saccharomyces carlsbergensis and transition state analysis (2009), Chem. Biol. Interact., 178, 16-23.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expressed in Escherichia coli DH5alpha cells Saccharomyces cerevisiae
1.1.1.1 expressed in Escherichia coli DH5alpha cells Saccharomyces pastorianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 2 3 benzaldehyde in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C Saccharomyces pastorianus
1.1.1.1 2.9
-
 p-Methoxybenzaldehyde isozyme ADH1, in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C Saccharomyces cerevisiae
1.1.1.1 3.4
-
 p-Methoxybenzaldehyde in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C Saccharomyces pastorianus
1.1.1.1 5.9
-
 p-Methoxybenzaldehyde isozyme ADH2, in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C Saccharomyces cerevisiae
1.1.1.1 6.9
-
 p-methoxybenzyl alcohol isozyme ADH2, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C Saccharomyces cerevisiae
1.1.1.1 11
-
 p-methoxybenzyl alcohol isozyme ADH1, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C Saccharomyces cerevisiae
1.1.1.1 14
-
 benzaldehyde isozyme ADH2, in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C Saccharomyces cerevisiae
1.1.1.1 22
-
 p-methoxybenzyl alcohol in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C Saccharomyces pastorianus
1.1.1.1 24
-
 benzyl alcohol in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C Saccharomyces pastorianus
1.1.1.1 33
-
 benzaldehyde isozyme ADH1, in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C Saccharomyces cerevisiae
1.1.1.1 34
-
 benzyl alcohol isozyme ADH1, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C Saccharomyces cerevisiae
1.1.1.1 49
-
 benzyl alcohol isozyme ADH2, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.1 cytoplasm
-
 Saccharomyces cerevisiae 5737
-
1.1.1.1 cytoplasm
-
 Saccharomyces pastorianus 5737
-
1.1.1.B3 cytoplasm
-
 Saccharomyces cerevisiae 5737
-
1.1.1.B4 cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Saccharomyces cerevisiae
-
-
-
1.1.1.1 Saccharomyces pastorianus B6UQD0
-
-
1.1.1.1 Saccharomyces pastorianus Y379-50 B6UQD0
-
-
1.1.1.B3 Saccharomyces cerevisiae
-
-
-
1.1.1.B3 Saccharomyces pastorianus
-
-
-
1.1.1.B3 Saccharomyces pastorianus Y379-50
-
-
-
1.1.1.B4 Saccharomyces cerevisiae
-
-
-
1.1.1.B4 Saccharomyces pastorianus
-
-
-
1.1.1.B4 Saccharomyces pastorianus Y379-50
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 DEAE-Sepharose CL-4B column chromatograhy and octyl-Sepharose column chromatography Saccharomyces cerevisiae
1.1.1.1 DEAE-Sepharose CL-4B column chromatograhy and octyl-Sepharose column chromatography Saccharomyces pastorianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 (R)-2-butanol + NAD+
-
 Saccharomyces cerevisiae 2-butanone + NADH + H+
-
 ?
1.1.1.1 (R)-2-butanol + NAD+
-
 Saccharomyces pastorianus 2-butanone + NADH + H+
-
 ?
1.1.1.1 (R)-2-butanol + NAD+
-
 Saccharomyces pastorianus Y379-50 2-butanone + NADH + H+
-
 ?
1.1.1.1 (R,S)-2-methylbutan-1-ol + NAD+
-
 Saccharomyces cerevisiae (R,S)-2-methyl-butan-1-one + NADH + H+
-
 ?
1.1.1.1 (R,S)-2-methylbutan-1-ol + NAD+
-
 Saccharomyces pastorianus (R,S)-2-methyl-butan-1-one + NADH + H+
-
 ?
1.1.1.1 (R,S)-2-methylbutan-1-ol + NAD+
-
 Saccharomyces pastorianus Y379-50 (R,S)-2-methyl-butan-1-one + NADH + H+
-
 ?
1.1.1.1 (S)-2-butanol + NAD+
-
 Saccharomyces cerevisiae 2-butanone + NADH + H+
-
 ?
1.1.1.1 (S)-2-butanol + NAD+
-
 Saccharomyces pastorianus 2-butanone + NADH + H+
-
 ?
1.1.1.1 (S)-2-butanol + NAD+
-
 Saccharomyces pastorianus Y379-50 2-butanone + NADH + H+
-
 ?
1.1.1.1 (S)-2-methylbutan-1-ol + NAD+
-
 Saccharomyces cerevisiae (S)-2-methyl-butanal + NADH + H+
-
 ?
1.1.1.1 (S)-2-methylbutan-1-ol + NAD+
-
 Saccharomyces pastorianus (S)-2-methyl-butanal + NADH + H+
-
 ?
1.1.1.1 2-methylpropan-1-ol + NAD+
-
 Saccharomyces cerevisiae 2-methyl-propan-1-one + NADH + H+
-
 ?
1.1.1.1 2-methylpropan-1-ol + NAD+
-
 Saccharomyces pastorianus 2-methyl-propan-1-one + NADH + H+
-
 ?
1.1.1.1 2-propanol + NAD+
-
 Saccharomyces cerevisiae acetone + NADH + H+
-
 ?
1.1.1.1 2-propanol + NAD+
-
 Saccharomyces pastorianus acetone + NADH + H+
-
 ?
1.1.1.1 2-propanol + NAD+
-
 Saccharomyces pastorianus Y379-50 acetone + NADH + H+
-
 ?
1.1.1.1 3-methylbutan-1-ol + NAD+
-
 Saccharomyces cerevisiae 3-methyl-butan-1-one + NADH + H+
-
 ?
1.1.1.1 3-methylbutan-1-ol + NAD+
-
 Saccharomyces pastorianus 3-methyl-butan-1-one + NADH + H+
-
 ?
1.1.1.1 benzyl alcohol + NAD+
-
 Saccharomyces cerevisiae benzaldehyde + NADH + H+
-
 r
1.1.1.1 benzyl alcohol + NAD+
-
 Saccharomyces pastorianus benzaldehyde + NADH + H+
-
 r
1.1.1.1 benzyl alcohol + NAD+
-
 Saccharomyces pastorianus Y379-50 benzaldehyde + NADH + H+
-
 r
1.1.1.1 octanol + NAD+
-
 Saccharomyces cerevisiae octanal + NADH + H+
-
 ?
1.1.1.1 octanol + NAD+
-
 Saccharomyces pastorianus octanal + NADH + H+
-
 ?
1.1.1.1 p-methoxybenzyl alcohol + NAD+
-
 Saccharomyces cerevisiae p-methoxybenzaldehyde + NADH + H+
-
 r
1.1.1.1 p-methoxybenzyl alcohol + NAD+
-
 Saccharomyces pastorianus p-methoxybenzaldehyde + NADH + H+
-
 r
1.1.1.1 pentanol + NAD+
-
 Saccharomyces cerevisiae pentanal + NADH + H+
-
 ?
1.1.1.1 pentanol + NAD+
-
 Saccharomyces pastorianus pentanal + NADH + H+
-
 ?
1.1.1.B3 (S)-2-butanol + NAD+
-
 Saccharomyces pastorianus 2-butanone + NADH + H+
-
 ?
1.1.1.B3 (S)-2-butanol + NAD+
-
 Saccharomyces cerevisiae 2-butanone + NADH + H+
-
 ?
1.1.1.B3 (S)-2-butanol + NAD+
-
 Saccharomyces pastorianus Y379-50 2-butanone + NADH + H+
-
 ?
1.1.1.B4 (R)-2-butanol + NAD+
-
 Saccharomyces pastorianus 2-butanone + NADH + H+
-
 ?
1.1.1.B4 (R)-2-butanol + NAD+
-
 Saccharomyces cerevisiae 2-butanone + NADH + H+
-
 ?
1.1.1.B4 (R)-2-butanol + NAD+
-
 Saccharomyces pastorianus Y379-50 2-butanone + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH1
-
 Saccharomyces pastorianus
1.1.1.1 ADH1 isozyme Saccharomyces cerevisiae
1.1.1.1 ADH2 isozyme Saccharomyces cerevisiae
1.1.1.B3 ADH1
-
 Saccharomyces pastorianus
1.1.1.B3 ADH1 isozyme Saccharomyces cerevisiae
1.1.1.B3 ADH2 isozyme Saccharomyces cerevisiae
1.1.1.B4 ADH1
-
 Saccharomyces pastorianus
1.1.1.B4 ADH1 isozyme Saccharomyces cerevisiae
1.1.1.B4 ADH2 isozyme Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Saccharomyces cerevisiae
1.1.1.1 NAD+
-
 Saccharomyces pastorianus
1.1.1.B3 NAD+
-
 Saccharomyces pastorianus
1.1.1.B3 NAD+
-
 Saccharomyces cerevisiae
1.1.1.B4 NAD+
-
 Saccharomyces pastorianus
1.1.1.B4 NAD+
-
 Saccharomyces cerevisiae